1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and RuralAffairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea FisheriesResearch Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. College ofFisheries, Tianjin Agricultural University, Tianjin 300384, China; 3. Shenzhen Academy ofInspection and Quarantine, Shenzhen 518010, China
Abstract：The changes in Na+/K+-ATPase and Ca2+-ATPase activities in gill and the contents of free amino acid (FAA) in muscle and gill were monitored in Pacific white shrimp Litopenaeus vannamei with body weight of (6.77±0.51) g reared in 200 L fiberglass barrels at initial stocking density of 50 per barrel under two temperatures (normally 30 ℃ and acute increase from 30 ℃ to 33 ℃ within 1 h) × 4 ammonia nitrogen concentrations (0, 5, 15, and 25 mg/L) adjusted with ammonium chloride solution for 72 h to evaluate the effects of ammonia-nitrogen stress on the physiological osmoregulation of Pacific white shrimp under acute warming temperature. The results showed that the activities of Na+/K+-ATPase and Ca2+-ATPase were found to be significantly increased in 25 mg/L and 5 mg/L ammonia groups at 30 ℃(P<0.05), significant increase in activities of Na+/K+-ATPaes and Ca2+-ATPase in all ammonia groups at 33 ℃(P<0.05). In the same ammonia concentration, there were significant differences in the activities of Na+/K+-ATPase and Ca2+-ATPase between 30 ℃ and 33 ℃ groups, significantly lower enzyme activity in the 33 ℃ group than that in the 30 ℃ group at 25 mg/L ammonia concentration (P<0.05). In gill and muscle, the total contents of FAA were significantly lower in the 33 ℃ group than that in 30 ℃ group at ammonia concentration of 15 mg/L and 25 mg/L (P<0.05). Under ammonia stress, alanine level in muscle was increased significantly in 30 ℃ and 33 ℃ groups with increasing ammonia concentration (P<0.05), and significantly higher at 33 ℃ than at 30 ℃ group (P<0.05).The findings indicated that ammonia stress sharply affected Na+/K+-ATPase and Ca2+-ATPase activities as well as the contents of FAA under acute warming temperature,leading to dysfunction of the physiological osmoregulation of Pacific white shrimp.
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2021, Vol. 40 
