Abstract：The process of saponin extracting from sea cucumber Apostichopus japonicus was optimized to provide reference for the quality evaluation. Cladoloside A was used for evaluation index, and three independent variable factors including microwave time, temperature and ethanol concentrations were examined. The extraction process of cladoloside A was optimized by single factor experiment coupled with Box-Benhken response surface method. Results showed that based on the regression result and considering the feasibility of experiment conduction, the following independent variable values were chosen as the optimized conditions for the extraction of saponin: extraction time of 10 min, extraction temperature of 45 ℃ and 25 mL 70% ethanol under 400 W. The response surface method was used to optimize the extraction of saponins, and it was feasible to provide technical support for the quality control of sea cucumber.
[1]Kalinin V I, Avilov S A, Silchenko A S, et al. Triterpene glycosides of sea cucumbers (Holothuroidea,Echinodermata) as taxonomic markers[J].Natural Product Communications,2015,10(1):21-26. [2]Nigrelli R, Zahl P A. Some biological characteristics of holothurin[J].Experimental Biology and Medicine,1952,81(2):379-380. [3]Zhao Q, Liu Z D, Xue Y, et al. Ds-echinoside A,a new triterpene glycoside derived from sea cucumber,exhibits antimetastatic activity via the inhibition of NF-κB-dependent MMP-9 and VEGF expressions[J].Journal of Zhejiang University: Science B,2011,12(7):534-544. [4]Bahrami Y, Franco C. Acetylated triterpene glycosides and their biological activity from Holothuroidea reported in the past six decades[J].Marine Drugs,2016,14(8):147. [5]Yuan W H, Yi Y H, Xue, M, et al. Two anifungal active triterpene glycosides from sea cucumber holothuria(Microthele) axiloga[J].Chinese Journal of Natural Medicines,2008,6(2):105-108. [6]阮伟达,苏永昌,吴成业.海参皂苷的研究现状[J].福建水产,2011,33(2):74-78. [7]刘旭朝,孙稚颖,周凤琴.海参的化学成分及药理作用研究进展[J].辽宁中医药大学学报,2016,18(4):64-68. [8]韦豪华,张红玲,李兴太.海参化学成分及生物活性研究进展[J].食品安全质量检测学报,2017,8(6):2054-2061. [9]袁炜辉,李赫宇,李倩,等.海参皂苷的分离提取及分析方法研究进展[J].食品研究与开发,2017,38(7):220-224. [10]于林芳,薛长湖,董平,等.八种海参体内皂苷类成分的SPE-HPLC分析比较[J].分析试验室,2011,30(1):13-15. [11]于林芳,王超,王玉明,等.大孔树脂纯化革皮氏海参总皂苷工艺[J].食品科学,2011,32(12):1-4. [12]丛日山,袁文鹏,樊廷俊,等.仿刺参水溶性海参皂苷的分离制备及抗真菌活性的研究[J].中国海洋大学学报(自然科学版),2006,36(6):959-964. [13]赵丽蓉,罗汉,相英龙,等.Box-Benhken响应面法优化微波提取黄精总皂苷的条件[J].中国现代中药,2018,20(8):1010-1015. [14]兰艳素,李长江,亓昭鹏,等.响应面法优化微波辅助提取广豆根中总黄酮工艺[J].中成药,2015,37(6):1360-1363. [15]刘梦迪,梁德勤,金传山,等.Box-Benhken响应面法优化超声提取天冬中皂苷的工艺[J].安徽中医药大学学报,2016,35(5):89-93. [16]薛长湖,于林芳,董平,等.青岛仿刺参中3种海参皂苷的分离纯化及结构鉴定[J].中国海洋大学学报(自然科学版),2010,40(8):60-65. [17]郭双双,杨利民,张一鸣,等.微波辅助萃取人参总皂苷与单体皂苷含量分析[J].食品科学,2015,36(2):1-6. [18]欧小宏,王晓,杨雁,等.正交试验法优选三七皂苷成分超声提取工艺[J].中国现代中药,2012,14(4):48-52. [19]郭丹丹,成乐琴,李玲,等.响应曲面法优化微波辅助提取人参须根粉中人参皂苷Rg5的工艺研究[J].食品工业科技,2017,38(11):229-234. [20]王威,孙晓丽.响应面法优化剪刀股中总皂苷的提取工艺[J].中国现代中药,2017,19(7):1021-1025. [21]黄玉兰,殷奎德,岳才军.响应面法优化微波提取绞股蓝愈伤组织中人参皂苷Rb1的工艺研究[J].激光生物学报,2013,22(6):521-527. [22]Karacabey E, Mazza G. Optimisation of antioxidant activity of grape cane extracts using response surface methodology[J].Food Chemistry,2010,119(1):343-348. [23]李莉,张赛,何强,等.响应面法在试验设计与优化中的应用[J].实验室研究与探索,2015,34(8):41-45. [24]张一非,陈艳红,伍菱,等.响应面优化红毛藻多糖提取工艺及抗光氧化活性[J].水产科学,2019,38(6):749-758. [25]Majumder A, Singh A, Goyal A. Application of response surface methodology for glucan production from Leuconostoc dextranicum and its structural characterization[J].Carbohydrate Polymers,2009,75(1):150-156. [26]逯家辉,王迪,郭伟良,等.响应面法优化八角茴香中莽草酸的超声波提取工艺研究[J].林产化学与工业,2008,28(1):87-91. [27]逯家辉,姜鑫,李昊龙,等.应用响应面法优化超声波法提取甘草中总黄酮的工艺[J].吉林大学学报(工学版),2008,38(增刊2):293-298. [28]邓恒为,周永灿,谢珍玉,等.抗哈氏弧菌中草药筛选及其活性成分提取工艺的优化[J].水产科学,2013,32(5):249-255. [29]彭雍博,汪秋宽,任丹丹,等.铜藻主要活性物质的提取及其功能[J].水产科学,2017,36(4):531-537. [30]王琴,罗洁莹,柳建良,等.响应面法优化超声—微波协同辅助提取金柚幼果总黄酮工艺[J].食品研究与开发,2020,41(2):83-91.
2021, Vol. 40 
