Effect of Temperature on Embryonic Developmentof Sinibrama taeniatus
WANG Yali, XIE Chongyou, YIN Min, CAI Ruiyu, WANG Zhijian
Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratoryof Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
Abstract：In this study, fertilized eggs of Sinibrama taeniatus were incubated in a 45 cm×25 cm×25 cm aquarium at water temperature of 16, 19, 22, 25, 28 and 31 ℃ to explore the effect of temperature on the embryonic development of the fish. It was found that the embryonic development process of S.taeniatus was divided into 8 stages including fertilized egg stage, uplift of blastoderm stage, cleavage stage, blastula stage, gastrula stage, neurula stage, organogenesis stage and hatching stage. It took 44.83 h for hatching of the embryo at water temperature of (25±0.5) ℃. However, the embryos were all died at gastrula stage at water temperature of 16 ℃, with hatching rate of only 30.00% at water temperature of 19 ℃, with the abnormality rate of 87.19% , significantly higher than that in other four groups (P<0.05). The incubation period was shortened at water temperature ranging from 19 ℃ to 31 ℃, significant difference among different temperatures (P<0.05), organogenesis stage being the longest stage in the 8 stages, accounting for 72.53%—77.07% of incubation period. Meanwhile, the abnormality rate was increased with increasing temperature, slightly twice at 31 ℃ as at 28 ℃. The maximal fertilization rate, hatching rate and survival rate and minimal abnormality were observed at water temperature varying from 22 ℃ to 28 ℃, with the optimal incubating temperature of 25 ℃. The regression equation between egg size (y)and the incubation period (x) was described as: y=49.56-44.36x+47.92x2+47.92x2 (r2=0.996) in embryonic development of S.taeniatus at (19±1) ℃, with biological zero point of 12.69 ℃ and the effective accumulative temperature of 522.35—595.11 ℃·h.
[1]陈宜瑜. 中国动物志:硬骨鱼纲鲤形目(中卷)[M]. 北京:科学出版社,1998:146-147. [2]张春光,赵亚辉. 中国内陆鱼类物种与分布[M]. 北京:科学出版社,2016:63-64. [3]李斌,解崇友,张婷,等. 岷江眉山段四川华鳊的繁殖生物学资料[J]. 动物学杂志,2015,50(4):563-570. [4]解崇友,倪露芸,吴迪,等. 岷江眉山段四川华鳊年龄与生长[J]. 四川动物,2016,35(1):78-83. [5]Li W T, Shao K, Xiong M H, et al. The complete mitochondrial genome of Sinibrama taeniatus (Cyprinidae:Cultrinae)[J]. Mitochondrial DNA,2014,27(3):1-2. [6]尹敏,解崇友,金丽,等. 四川华鳊卵子发生的显微结构观察[J]. 淡水渔业,2019,49(2):27-32. [7]尹敏,解崇友,蒲德永,等. 四川华鳊卵子发生的显微结构观察[J]. 南方水产科学,2019,15(2):127-132. [8]Herzig A, Winkler H. The influence of temperature on the embryonic development of three cyprinid fishes,Abramis brama, Chalcalburnus chalcoides mento and Vimba vimba[J]. Journal of Fish Biology,2010,28(2):171-181. [9]Polo A, Yúfera M, Pascual E. Effects of temperature on egg and larval development of Sparus aurata L.[J]. Aquaculture,1991,92:367-375. [10]Zacharia S, Kakati V S. Optimal salinity and temperature for early developmental stages of Penaeus merguiensis De man[J]. Aquaculture,2004,232(1):373-382. [11]Bhattacharya H, Zhang S C, Wang Y J. Embryonic development of the rosy barb Puntius conchonius Hamilton 1822(Cyprinidae)[J]. Tropical Zoology,2005,18(1):25-37. [12]朱成德.不同温度对太湖短吻银鱼秋季孵化的试验研究[J].生态学报,1984,4(1):65-72. [13]刘艳超,刘海平,刘书蕴,等.温度对尖裸鲤胚胎发育及其仔稚鱼生长性状的影响[J].动物学杂志,2018,53(6):910-923. [14]刘艳超,范丽卿,何文佳,等.温度对拉萨裸裂尻鱼胚胎发育的影响[J].高原农业,2019,3(2):168-175. [15]杨君兴,潘晓赋,陈小勇,等.中国淡水鱼类人工增殖放流现状[J].动物学研究,2013,34(4):267-280. [16]刘筠. 中国养殖鱼类繁殖生理学[M]. 北京:农业出版社,1993:81-93. [17]刘阳,朱挺兵,吴兴兵,等. 短须裂腹鱼胚胎及早期仔鱼发育观察[J]. 水产科学,2015,34(11):683-689. [18]申安华,李光华,赵树海,等. 光唇裂腹鱼胚胎发育与仔鱼早期发育的研究[J]. 水生态学杂志,2013,34(6):76-80. [19]黄洪贵. 中华倒刺鲃胚胎与仔鱼发育的观察[J]. 江西农业大学学报,2009,31(6):1087-1092. [20]韩耀全,何安尤,蓝家湖,等. 乌原鲤的胚胎发育特征[J]. 水产科学,2018,37(3):368-373. [21]吴青,王强,蔡礼明,等. 齐口裂腹鱼的胚胎发育和仔鱼的早期发育[J]. 大连水产学院学报,2004,19(3):218-221. [22]王磊,施文瑞,宋东蓥,等. 草金鱼胚胎与胚后发育观察[J]. 水产科学,2018,37(6):742-748. [23]顾志敏,朱俊杰,贾永义,等. 太湖翘嘴红鲌胚胎发育及胚后发育观察[J]. 中国水产科学,2008,15(2):204-214. [24]李池陶,徐伟,王进,等. 池塘养殖兴凯湖翘嘴鲌的人工繁殖和胚胎发育观察[J]. 大连水产学院学报,2008,23(2):87-91. [25]胡振禧,黄洪贵,吴妹英,等. 温度对斑鳜胚胎发育的影响[J]. 淡水渔业,2014(3):104-107. [26]李忻林,董洪坪,王婷,等. 温度对斑马鱼胚胎发育的影响[J]. 四川动物,2014,33(6):829-835. [27]许晖,钟必华. 加州鲈鱼的人工繁殖和胚胎发育[J]. 淡水渔业,1987(1):33-34. [28]Bobe J, Labbe C. Egg and sperm quality in fish[J]. General and Comparative Endocrinology,2009,165(3):535-548. [29]钟全福. 黑莓鲈胚胎发育观察及温度对胚胎发育的影响[J]. 福建水产,2014,36(5):333-343. [30]刘希良,宾石玉,王开卓,等. 翘嘴鳜的人工繁殖与胚胎发育观察[J]. 广西师范大学学报:自然科学版,2013,31(2):100-105. [31]薛凌展. 温度对异育银鲫“中科3号”胚胎发育的影响[J]. 福建师范大学学报:自然科学版,2014,30(4):76-83. [32]万远,占阳,欧阳珊,等. 胭脂鱼胚胎及仔鱼早期发育观察[J]. 南昌大学学报:理科版,2013,37(1):78-82. [33]赵健蓉,解崇友,蔡瑞钰,等. 云南盘鮈人工繁殖及胚胎发育初步观察[J]. 南方水产科学,2017,13(5):124-128. [34]Leblanc C A, Kristjansson B K, Skulason S. The importance of egg size and egg energy density for early size patterns and performance of Arctic charr Salvelinus alpinus[J]. Aquaculture Research,2016,47(4):1100-1111. [35]李庆芬. 基础生态学[M]. 北京:高等教育出版社,2002:25. [36]陈凤梅,胡家会,王曰文,等. 温度对玫瑰无须鲃胚胎发育的影响[J]. 淡水渔业,2013,43(1):24-27. [37]徐钢春,张守领,顾若波. 温度、盐度对似刺鳊鮈胚胎发育过程的影响[J]. 淡水渔业,2012,42(1):72-75. [38]何利君. 温度对鳜鱼胚胎发育的影响[J]. 四川畜牧兽医学院学报,1999,13(4):19-22. [39]李文豪,冉皓宇,陈良标. 低温胁迫导致斑马鱼尾部发育畸形的分子机制[J]. 基因组学与应用生物学,2018,37(6):2383-2388. [40]袁宗勤,于燕光,丛日浩,等. 温度对斑马鱼胚胎发育的影响研究[J]. 现代农业科学,2009,16(4):188-189. [41]王宏田,张培军. 环境因子对海产鱼类受精卵及早期仔鱼发育的影响[J]. 海洋科学,1998,22(4):50-52. [42]吴利敏,袁淑仪,赵晨华,等. 不同温度对淇河鲫胚胎发育的影响[J]. 山西农业大学学报:自然科学版,2018,38(7):50-55.
2020, Vol. 39 
