Abstract：In many countries, food safety limit standards have been established for florfenicol residues in food and the corresponding drug withdrawal period has been specified with the extensive usage of antibiotics. In this study, exposure experiment was conducted for 7 days administration of florfenicol-containing diet at 10 mg/(kg·d) and 14 days discontinuation of the drug in recirculating aquaculture systems (RAS). HPLC was used to detect the residual florfenicol in RAS, muscle and liver tissues of toothed-tongue seabass Dicentrarchus labrax. The results showed that the concentration of florfenicol in the units of RAS (Protein skimmer, biofilter, and UV disinfection device) was increased with different degrees. The maximum value of florfenicol was observed at the 7th day of administration. Then the residual concentration of florfenicol was gradually decreased to the pre-treated level at the 14th day of drug withdrawal. At the same time, the contribution of florfenicol removal in different treatment units: UV disinfection device>protein skimmer>biofilter. Meanwhile, the residual level of florfenicol in fish tissues was decreased with the prolonged withdrawal time, and the residual florfenicol in muscle tissue was always higher than that in florfenicol in liver tissue. After 7 days withdrawal, the residual amount in liver and muscle tissues was lower than the national standard of 1000 μg/kg. The findings will provide a theoretical basis for the process design of RAS, and contribute to the sustainable and healthy development of aquaculture industry.
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2020, Vol. 39 
