Abstract：The morphometric differentiation of wild spotted mandarinfish Siniperca kneri among three different sampling sites of Yuanjiang, Liujiang and Wujiang rivers in Guizhou province were assessed using multivariate analysis methods based on the measured morphological data and the truss network. After data standardization and parameter selection for original data,the statistical analysis was performed on 12 proportional traits and 12 countable traits.The results showed that among the three populations there were significant differences among the nine countable traits in the single factor analysis (P<0.05). Six principal components were constructed, with cumulative contributory ratio of 72.668%. The top three principal components were mainly affected by characters, including body width, caudal peduncle shape, and distance between the eyes. A stepwise discriminant method was used to establish the characteristic discriminant functions of the three stocks, which revealed that the total accuracy rate of discrimination by the cross-validated method was 81.5%. Based on the morphological clustering map, the morphological relationship between the Yuanjiang and Liujiang populations was relatively close, then gather with Wujiang population. The difference coefficient analysis indicated that the difference among the three populations in the caudal peduncle length/body length traits reached the subspecies level (CD>1.28). It is concluded that there were some differences in morphology among the populations, the difference between Liujiang and Yuanjiang was small, but Wujiang was quite different. The established discriminant equation can distinguish the three spotted mandarinfish populations. The findings can be used to identify the three mandarinfish populations in the future, and also provide a theoretical basis for population conservation and breeding.
[1]Bookstein F L. Foundations of morphometrics[J]. Annual Review of Ecology & Systematics,1982,13(13):451-470. [2]Humphries J M, Bookstein F L, Chernoff B, et al. Multivariate discrimination by shape in relation to size[J]. Systematic Biology,1981,30(3):291-308. [3]Cadrin S X. Advances in morphometric identification of fishery stocks[J]. Reviews in Fish Biology & Fisheries,2000,10(1):91-112. [4]李思发,李晨虹. 尼罗罗非鱼品系间形态差异分析[J]. 动物学报,1998,44(4):450-457. [5]Bagherian A, Rahmani H. Morphological differentiation between two populations of the Shemaya, Chalcalburnus chalcoides: a geometrical morphometric approach[J]. Zoology in the Middle East,2007,40(1):53-62. [6]刘楚珠,严利平,李建生,等. 基于框架法的东黄海日本鲭产卵种群形态差异分析[J]. 中国水产科学,2011,18(4):908-917. [7]周才武,杨青,蔡德霖. 鳜亚科 SINIPERCIDAE 鱼类的分类整理和地理分布[J]. 动物学研究,1988,9(2):113-125. [8]李思忠. 鳜亚科鱼类地理分布的研究[J]. 动物学杂志,1991,26(4):40-44. [9]王伟伟,赵金良,李思发. 我国斑鳜不同种群mtDNA控制区序列的遗传变异[J]. 上海水产大学学报,2006,15(4):398-402. [10]王伟伟,赵金良,李思发. 我国斑鳜六个种群mtDNA Cyt b序列的遗传变异[J]. 动物学研究,2006,27(6):589-593. [11]周文漪. 基于线粒体细胞色素b的7水系斑鳜遗传多样性分析[D]. 广州:暨南大学,2014:1-68. [12]江林源,黄芳洁,丁绍雄,等. 西江斑鳜的形态学特征研究[J]. 水产学杂志,2009,22(2):46-48. [13]许淼洋,郭金涛,赵金良,等. 斑鳜(♀)×鳜鱼(♂)杂交子一代、子二代的形态特征分析[J]. 水产科学,2013,32(11):636-640. [14]张进,梁旭方,易提林,等. 翘嘴鳜与斑鳜杂交F1代食性驯化及主要形态性状的通径分析[J]. 水产科学,2013,32(1):1-6. [15]伍律,赵执桴,郑建州,等. 贵州鱼类志[M]. 贵阳: 贵州人民出版社,1989:259-266. [16]刘楚珠,严利平,李建生,等. 基于框架法的东黄海日本鲭产卵群体形态差异分析[J]. 中国水产科学,2011,18(4):908-917. [17]陈薛伟杰,郭健康,杨志,等. 贵州境内3个野生大眼鳜种群的形态差异[J]. 中国水产科学,2018,25(1):34-43. [18]Mohaddasi M, Shabanipour N, Abdolmaleki S. Morphometric variation among four populations of shemaya (Alburnus chalcoides) in the south of Caspian sea using truss network[J]. The Journal of Basic & Applied Zoology,2013,66(2):87-92. [19]杨慧荣,欧阳徘徊,李桂峰,等. 珠江流域3个野生大眼鳜种群的形态差异[J]. 中国水产科学,2016,23(2):447-457. [20]杜强,贾丽艳,严先锋. SPSS统计分析—从入门到精通[M]. 2版. 北京:人民邮电出版社,2014:113-339. [21]Mayr E, Linsle E G, Usinger R L. Methods and principles of Systematic Zoology[M]. New York: McGraw-Hill,1953:125-154. [22]蒙彦晓,王桂华,熊冬梅,等. 基于形态学差异探讨秦岭细鳞鲑亚种有效性问题[J]. 水生生物学报,2018,42(3):102-112. [23]林艳红,安苗,姜海波. 3种裂腹鱼的形态差异研究[J]. 贵州农业科学,2010,38(10):121-126. [24]Tudela S. Morphological variability in a Mediterranean, genetically homogeneous population of the European anchovy, Engraulis encrasicolus [J]. Fisheries Research,1999,42(3):229-243. [25]任明荣. 逐步判别在鱼类形态判别上的应用[J]. 上海海洋大学学报,1998,7(3):258-264. [26]舒琥,江小璐,杨华强,等. 华南地区7个大刺鳅野生种群的形态差异分析[J]. 广州大学学报:自然科学版,2017,16(3):8-13. [27]Kinsey S T, Orsoy T, Bert T M, et al. Population structure of the Spanish sardine Sardinella auriia: natural morphological variation in a genetically homogenous population[J]. Marine Biology,1994,118(2):309-317. [28]陈杰,李福贵,黄创新,等. 不同鳊鲂属鱼类种群的形态差异分析[J]. 上海海洋大学学报,2014,23(3):388-394. [29]Scribner K T, Evans J E, Morreale S J, et al. Genetic divergence among populations of the yellow-bellied slider turtle (Pseudemys scripta) separated by aquatic and terrestrial habitats[J]. Copeia,1986,1986(3):691-700. [30]王伟伟,赵金良,李思发. 斑鳜不同地理种群遗传变异的AFLP分析[J]. 水生生物学报,2009,33(2):304-309.
2020, Vol. 39 
