Abstract：A method was established for the detection of rifampicin residues in fishery products by ultra performance liquid chromatography-tandem mass spectrometry with dispersive solid phase extraction. The samples were extracted with acetonitrile-dichloromethane (6∶4, V/V) , purified by a mixture of 100 mg C18 and 100 mg ethylenediamine-N-propylsilane (PSA), and then eluted with 3 mmol/L ammonium acetate solution (containing 0.05% formic acid). The electrospray ionization mass spectrometry was operated in the positive mode using multiple reaction monitoring (MRM), and the target compound was quantified by the internal standard method. The results showed that the calibration curve for rifampicin exhibited good linear relationship in the range of 1—20 μg/kg with correlation coefficients (r) of more than 0.9986, with detection limit of 0.3 μg/kg and the quantification limit of 1.0 μg/kg. The average recoveries for Nile tilapia hybrid Oreochromis aureus×O.niloticus, Pacific white shrimp Litopenaeus vannamei and Chinese soft shelled turtle Trionyx sinensis were ranged from 90.17%—101.07% with relative standard deviations (RSDs) of 3.11%—7.66%. The method is suitable for the detection and confirmation of rifampicin in fishery products due to its simplicity, rapidity, and high accuracy, and provides convenient and effective technical support for the supervision of illegal use of rifampicin.
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2020, Vol. 39 
