Abstract：The microflora community structure of sediment was monthly determined and analyzed in the samples of surface sediment (about 200 g) collected in three intensive grass carp (Ctenopharyngodon idellus) culture ponds in Jiangxiang Town, Nanchang County, Jiangxi Province from April to November 2018 by Peterson mud collector by the Illumina MiSeq sequencing technique. It was found that the microflora in all samples were classified into 54—60 phyla, among which Proteobacteria was the maximal dominant phyla, and the other dominant phyla were varied with months. There was no significant difference in the structure of microbial community in each month, while the main species were different in October from other months, with the peak of species abundance in May and November, and the maximal diversity in May. The core species included the anaerobic bacteria Geobacter and Anaeromyxobacter, and the facultative anaerobes Desulfatlans, Candidatus Competibacter, Dechloromonas and Crenothrix. These species bacteria reduced organic matter and sulfide under anaerobic or low dissolved oxygen conditions. Except in October, no significant difference in core species was observed in all months. However, the abundance of species is varied with month. In conclusion, the microflora structure in intensive grass carp culture pond sediments was relatively stable, with slow successive evolution, and increase in bottom dissolved oxygen level is the most important measure to repair the pond environment.
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2020, Vol. 39 
