Abstract：MiSeq high-throughput sequencing technology and bioinformatics analysis were used to construct gene sequencing libraries of intestinal microflora structure in six samples in wild and cultured Schizothorax o'connori with body weight of about 1 kgto compare diversity differences in structural and biological richness in intestinal microflora between wild and cultured S. o'connori. The results showed that the OTUs data of wild group samples Y1, Y2, Y3 and domesticated group samples C1, C2 and C3 were divided into 751, 469, 313, and 302, 259, 263, respectively, without significant difference in the richness and diversity of intestinal flora between wild and cultured animals (P>0.05). Actinobacteria and Proteobacteria were the dominant flora in the wild fish, while Cyanobacteria and Proteobacteria were the dominant flora in the cultured fish. Micrococcus and Aeromonas were the main taxonomic bacteria in the wild group, while Cyanobacteria were the main taxonomic bacteria in the cultured group. The findings provide a theoretical basis for the healthy culture of S. o'connori and the construction of micro-ecological control technology of culture environment.
[1]Mackie R I, White B A. Recent advances in rumen microbial ecology and metabolism:potential impact on nutrient output[J]. Journal of Dairy Science,1990,73(10):2971-2995. [2]Hegarty R S, Goopy J P, Herd R M, et al. Cattle selected for lower residual feed intake have reduced daily methane production[J]. Journal of Animal Science,2007,85(6):1479-1486. [3]Zhou M,Hernandez-sanabria E,Guan L L. Assessment of the microbial ecology of ruminal merhanogens in cattle with different feed efficiencies[J]. Applied and Environment Microbiology,2009,75(20):6524-6533. [4]Wu S G, Tian J Y, Wang G T, et al. Composition diversity and origin of the bacterial community in grass carp intestine[J]. Microbiology & Biotechnology,2012,28(5):2165-2174. [5]Romero J, Navarrete P. 16S rDNA-based analysis of dominant bacteria population associated with early life stages of coho salmon (Oncorhynchus kisutch)[J]. Microbial Ecology,2006,51(4):422-430. [6]李袆,郑伟,郑天凌. 海洋微生物多样性及其分子生态学研究进展[J]. 微生物学通报,2013,40(4):655-668. [7]Caporaso J G, Lauber C L, Walters W A, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample[J]. Proceedings of the National Academy of Sciences of the United States of America,2011,108(S1):4516-4522. [8]Youssef N, Sheik C S, Krumholz L R, et al. Comparison of species richness estimates obtained using nearly complete fragments and simulated pyrosequencing-generated fragments in 16S rRNA gene-based environmental surveys[J]. Applied and Environmental Microbiology,2009,75(16):5227-5236. [9]李晓然. 基于核糖体RNA高通量测序分析微生物群落结构[D]. 上海:复旦大学,2011. [10]陈毅峰,曹文宣. 裂腹鱼亚科鱼类.中国动物志硬骨鱼鲤形目(下卷)[M]. 北京:科学出版社,2000:273-388. [11]张涵,周涛,王岩. 综合养殖池塘中三角帆蚌和鱼类肠道细菌的组成[J]. 水生生物学报,2013,37(5):824-835. [12]夏耘,王一飞,郁二蒙,等. 生态基对草鱼生长性能、肠道及水体微生物的影响[J]. 水产学报,2014,38(9):1410-1420. [13]裴鹏兵,吴洁琼,梁宏豪,等. 生物净水栅对凡纳滨对虾肠道菌群组成的影响[J].水产科学,2018,37(3):301-308. [14]李学梅,余育和,解绶启,等. 三种室内饲养鱼类肠道微生物群落PCR-DGGE指纹分析[J]. 水生生物学报,2011,35(3):423-429. [15]王琴,熊邦喜,朱玉婷,等. 主养草鱼池塘三种混养模式下鱼类肠道菌群PCR-DGGE比较[J]. 农业生物技术学报,2012,20(3):308-315. [16]Olafsen J A. Interactions between fish larvae and bacteria in marine aquaculture[J]. Aquaculture,2001,200(1/2):223-247. [17]Cahill M M. Bacterial flora of fishes:a review[J]. Microbial Ecology,1990,19(1):21-41. [18]Munro P O, Barbour A, Blrkbeck T H. Comparison of the gut bacterial flora of start-feeding larval turbot reared under different conditions[J]. Journal of Applied Microbiology,2010,77(5):560-566. [19]Nicolas J L, Robic E, Ansquer D. Bacterial flora associated with a trophic chain consisting of microalgae, rotifers and turbot larvae:influence of bacteria on larval survival[J]. Aquaculture,1989,83(3):237-248. [20]季强. 异齿裂腹鱼食性的初步研究[J].水利渔业,2008,28(3):51-53. [21]陈孝煊,吴志新,周文豪. 鱼类消化道菌群的作用与影响因素研究进展[J].华中农业大学学报,2005,24(5):523-528. [22]Tanasomwang V, Muroga K. Intestinal microflora of larval and juvenile stages in Japanese flouder(Paralichthys olivaceus)[J]. Fish Pathology,1988,23(2):77-83. [23]王红宁,何明清,柳萍,等. 鲤肠道正常菌群的研究[J]. 水生生物学报,1994,18(4):354-359. [24]王建建,高权新,张晨捷,等. 野生鱼养殖银鲳消化道菌群结构中产菌酶的对比分析[J]. 水产学报,2014,38(11):1899-1909. [25]Holben W E, Williams P, Saarinen M, et al. Phylogenetic analysis of intestinal microflom in farmed and wild salmon[J]. Microbial Ecology,2002,44(2):175-185. [26]Li K J, Guan W B, Xu J L, et al. PCR-DGGE analysis of bacterial diversity of the intestinal system in eight kinds fishes from the Changjiang river esturary[J].Chinese Journal of Microecology,2007,19(3):267-269.
2020, Vol. 39 
