Abstract：In this paper, a ultra-performance liquid chromatography-linear ion trap mass spectrometry(UPLC-MS)method was established for the detection of hydrocortisone in coelomic fluid of juvenile and 3 year old sea cucumber Apostichopus japonicus. Extraction conditions and purification methods were optimized. Samples were extracted with 0.2% formic acid acetonitrile solution, and the extract was subjected to chromatographic separation on Captiva EMR-lipid solidphase extraction column. Quantification of the analytes was achieved by UPLC-MS using the external standard method, with the limit of detection (LOD) of 0.11 μg/kg, and the limit of quantification (LOQ) of 0.35 μg/kg. The average recoveries of hydrocortisone in blank samples at spiked levels of 20, 40, and 100 μg/L were ranged from 77% to 105%. This method had the advantages of small matrix interference and accurate, which can satisfy detection of hydrocortisone in sea cucumber.
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2020, Vol. 39 
