Acute Toxicity of Four Drugs Used in Aquaculture to Juvenile Leech Whitmania pigra
GUO Kun1,2, LUO Mingzhong1,2, RUAN Guoliang1, WEI Wei2, LI Rui2
1. Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, China; 2. State Key Laboratory for Quality and Safety of Agroproducts (in Preparation),
Abstract：Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China Juvenile leech Whitmania pigra with body weight of (0.062±0.006) g was placed in an 80 mesh bag of 10 cm×10 cm×5 cm fixed and submerged in a 40 cm×50 cm×30 cm plastic tank, and the acute toxicity of copper sulfate, povidone iodine, potassium permanganate and phoxim to juvenile leech was studied under water temperature of (25.0±1.5) ℃, pH 7.2—7.8 and dissolved oxygen level (DO) of (6.0±0.5) mg/L by a semi-static test method. The results showed that the 24 h median lethal concentration (LC50) was found to be 0.579 mg/L, with safe concentration of 0.053 mg/L for copper sulfate; 78.173 mg/L, with safe concentration of 15.367 mg/L, for povidone iodine; 3.956 mg/L, with safe concentration of 0.706 mg/L for potassium permanganate and 0.978 mg/L, with safe concentration of 0.183 mg/L for phoxim, the 48 h LC50 0.389 mg/L for copper sulfate, 67.898 mg/L for povidone iodine, 3.327 mg/L for potassium permanganate and 0.836 mg/L for phoxim; the 72 h LC50 0.280 mg/L for copper sulfate, 62.747 mg/L for povidone iodine, 2.703 mg/L for potassium permanganate and 0.611 mg/L for phoxim, and the 96 h LC50 0.241 mg/L for copper sulfate, 56.058 mg/L for povidone iodine, 2.313 mg/L for potassium permanganate and 0.493 mg/L for phoxim. According to the toxic classification standards published by state environmental protection administration, copper sulfate and phoxim were within the high toxic substances,potassium permanganate was within the medium toxic substances, and povidone iodine belonged to the low toxic substances for juvenile leech.
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2020, Vol. 39 
