Effects of Salinity Stress on Activities of Enzymes Related to Immune of Snail Babylonia areolata
TAN Chunming1,2, ZHAO Wang1,2, WU Kaichang1,2, WEN Weigeng1,2, YANG Rui1, CHEN Xu1,2, YU Gang1,2
1. Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China; 2. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Abstract：Behavior and activities of six enzymes related to immune were determined in juvenile snail Babylonia areolata with average body weight of (0.386±0.080) g exposed to a salinity of 18, 23, 28, 33 (control group) and 38 at water temperature of (26.0±1.0) ℃ for 6, 12, 24, 36, 48, 72 and 96 hours to evaluate the changes in activities of the enzymes. The results showed that the suitable salinity for growth of the snail was ranged from 20 to 35, and high mortality was observed at the salinity of below 15 or above 45. Both the increase in salt concentration and in exposure period led to have significant effects on the activities of the immune enzymes (P<0.05). Compared with the control group, the catalase activity showed a “suppression-induction” trend in the snail in salinity 18 and 23 groups, and an “induction-suppression-induction” in the salinity 28 and 38 groups. The consistent “induction-suppression” in acid phosphatase and alkaline phosphatase activities were found in the snail in each salinity group, and the same case was observed in total superoxide dismutase activity of the snail in salinity 38 group, in catalase activity in the same salinity group. The total superoxide dismutase activity showed the trend of “induction-suppression” in salinity 18, 23 and 28 groups. The trend of “suppression-induction-suppression” in glutathione peroxidase activity in each salinity group and the peroxidase activity in the salinity 18 and 38 groups were found. The peroxidase activity in salinity 23 and 28 groups was consistent with the total superoxide dismutase activity change in the same salinity. So, the salinity in the aquaculture water will have a greater impact on the growth and immune enzyme activity of the snail. The catalase, alkaline phosphatase, total superoxide dismutase, acid phosphatase and glutathione peroxidase activities were induced when B. areolata were subjected to mild stress, while their activities were inhibited by severe stress.
[1]程炜轩,梁旭方,符云,等. 高温季节鳜及饵料鱼池塘水质调查研究[J]. 南方水产科学,2011,7(4):43-48. [2]刘建勇,绍杰,卓健辉. 盐度对方斑东风螺耗氧率和排氨率的影响[J]. 热带海洋学报,2005,24(4):35-40. [3]杨章武,郑雅友,李正良,等. 低盐度对方斑东风螺摄食与生长的影响[J]. 应用海洋学学报,2006,25(1):36-40. [4]Ma Z H, Qin J G, Nie Z. Morphological changes of marine fish larvae and their nutrition need[G]//Pourali K, Raad V N. Larvae morphology, biology and life cycle. New York:Nova Science Publishers, Inc,2012:1-20. [5]Ma Z H. Food ingestion, prey selectivity, feeding incidence, and performance of yellowtail kingfish Seriola lalandi larvae under constant and varying temperatures[J]. Aquaculture International,2014,22(4):1317-1330. [6]袁春营,崔青曼. 盐度影响水产养殖动物生长发育的研究进展[J]. 水产科学,2004,23(5):41-42. [7]黄凯,王武,卢洁,等. 盐度对南美白对虾的生长及生化成分的影响[J]. 海洋科学,2004,28(9):20-25. [8]房子恒. 不同盐度对半滑舌鳎幼鱼生长的影响及其生理生态学机制的研究[D].青岛:中国海洋大学,2013. [9]Pillai B R, Diwan A D. Effects of acute salinity stress on oxygen consumption and ammonia excretion rates of the marine shrimp Metapenaeus monoceros[J]. Journal of Crustacean Biology,2002,22(1):45-52. [10]王瑞芳,庄平,黄晓荣,等. 渗透胁迫对中华绒螯蟹胚胎生化组成及消化酶活性的影响[J]. 中国水产科学,2013,20(2):299-307. [11]刘珊,李冰,张成锋,等. 硬骨鱼类渗透调控的研究进展[J]. 安徽农业科学,2011,39(20):12239-12242. [12]Chen J C, Lin C Y. Oxygen consumption and ammonia-N excretion of Penaeus chinensis juveniles exposed to ambient ammonia at different salinity levels[J]. Comparative Biochemistry & Physiology C Comparative Pharmacology & Toxicology,1992,102(2):287-291. [13]Squires E J, Haard N F, Feltham L A. Gastric proteases of the Greenland cod Gadus ogac. Ⅱ. Structural properties[J]. Biochemical Cell Biology,1986,64(3):215-222. [14]田相利,任晓伟,董双林,等. 温度和盐度对半滑舌鳎幼鱼消化酶活性的影响[J]. 中国海洋大学学报:自然科学版,2008,38(6):895-901. [15]柳旭东,王际英,张利民,等. 盐度对水产动物生长代谢与生殖发育相关指标影响的研究 [J]. 水产养殖,2009,30(4):42-46. [16]刘建勇,卓健辉. 温度和盐度对方斑东风螺胚胎发育的影响[J]. 广东海洋大学学报,2005,25(1):1-4. [17]Berger V J, Kharazova A D. Mechanisms of salinity adaptations in marine mollusks[J]. Hydrobiologia,1997,355(1/3):115-126. [18]林志华,柴雪良,方军,等. 硬壳蛤对环境因子适应性实验[J]. 宁波大学学报:自然科学版,2002,15(1):19-22. [19]Forcucci D, Lawrence J M. Effect of low salinity on the activity, feeding, growth and absorption efficiency of Luidia clathrata (Echinodermata: Asteroidea)[J]. Marine Biology,1986,92(3):315-321. [20]阎希柱,王桂忠,李少菁. 盐度对九孔鲍能量收支的影响[J]. 生态学杂志,2009,28(8):1520-1524. [21]陈泰霖. 低渗透压通过促进板状伪足的形成加速细胞缺口的关闭[D]. 南宁:广西医科大学,2017. [22]贾倩倩,吕为群. 低盐胁迫对褐牙鲆成鱼血浆渗透压、皮质醇、生长激素和催乳素的影响[J]. 上海海洋大学学报,2016,25(1):71-77. [23]廉颖,桂朗,张俊彬. 不同渗透压条件下金钱鱼肾原代细胞的差异蛋白分析[J]. 上海海洋大学学报,2018,10(22):1-12. [24]陈兴娟. 细胞膜电位调节细胞膜PIP2代谢的分子机制研究[D]. 石家庄:河北医科大学,2012. [25]李刚,唐学玺,窦勇,等. 盐度对刺参(Apostichopus japonicus)消化酶活力的影响[J]. 海洋环境科学,2011,30(1):61-63. [26]时少坤,王瑞璇,王江勇,等. 盐度胁迫对近江牡蛎几种免疫因子的影响[J]. 南方水产科学,2013,9(3):26-30. [27]廖雅丽,张晨捷,彭士明,等. 盐度对云纹石斑鱼抗氧化酶及溶菌酶活性的影响[J]. 上海海洋大学学报,2016,25(2):169-176. [28]Martine Z, Alvarezr M, Moralesa E, et al. Antioxidant defenses in fish: biotic and abiotic factors[J]. Reviews in Fish Biology Fisheries,2005,15(1/2):75-88. [29]张克烽,张子平,陈芸,等. 动物的抗氧化系统[J]. 动物学杂志,2007,42(2):153-160. [30]孙鹏,尹飞,彭士明,等. 盐度对条石鲷幼鱼肝脏抗氧化酶活力的影响[J]. 海洋渔业,2010,32(2):154-159. [31]王庚申,谢建军,施慧,等. 盐度对脊尾白虾生长及肝胰腺消化酶活力的影响[J]. 水产科学,2014,33(7):451-454. [32]陈宇锋,艾春香,林琼武,等. 盐度胁迫对锯缘青蟹血清及组织、器官中PO和SOD活性的影响[J]. 台湾海峡,2007,26(4):569-575. [33]宋晓楠,马峻峰,秦艳杰,等. 盐度骤降对菲律宾蛤仔抗氧化酶活力及组织结构的影响[J]. 农学学报,2013,3(1):50-56. [34]徐立红,张甬元,陈宜瑜. 分子生态毒理学研究进展及其在水环境保护中的意义[J]. 水生生物学报,1995,19(2):171-185. [35]汪家鑫,张钊,胡盼,等. 亚硝酸氮对红鳍东方鲀的毒性效应[J]. 广东海洋大学学报,2013,33(6):52-56.
2020, Vol. 39 
