Bacterial Community and Diversity Analysis in a Polyculture System of Sea Cucumber Apostichopus japonicus with Chinese Shrimp Fenneropenaeus chinensis, and Tiger Shrimp Penaeus monodon
GUAN Xiaoyan1,2, WANG Bai2, JIANG Jingwei2, TIAN Jiashen2, DONG Ying2, ZHOU Zunchun2, WANG Xuda2
1. College of Environment Science and Engineering, Dalian Maritime University, Dalian 116026, China; 2. Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China
Abstract：Sea cucumber Apostichopus japonicas was polycultured with Chinese shrimp Fenneropenaeus chinensis (30 000 ind/hm2), and tiger shrimp Penaeus monodon (7500 ind/hm2) in a 3.33 hm2 and a water depth of 1.5 m pond in coastal Liaoning Province in Liaodong Bay from June to September 2018, with catching and stocking in rotation and without feeding, and the changes in diversity and abundance of the bacterial community were quantitatively analyzed in the water samples collected around the 15th of each month in the polyculture pond through the high-throughput sequencing of 16S rRNA amplicon to explore the dynamic changes in diversity and composition structure of the microbial community in the polyculture pond during the culture period. The results showed that the most important bacteria in the polytrophic waters was Proteobacteria (51.5%), followed by Actinobacteria (23.6%), Bacteroidetes (11.3%) and Firmicutes (9.1%). Cluster analysis showed that there were significant differences in the bacterial communities in the pond waters between the early period of aquaculture (June to July) and the late period (from August to September): significant increase in the abundance and diversity of the microbial communities in the pond waters in the later period. The abundance of Actinomycota showed a trend of first decreasing and then increasing, while the abundance of the Firmicutes in the late stage of culture was significantly higher than that in the early stage of culture. The dominant phyla in the water of A.F.P ponds were Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. The relative abundance of Proteobacteria was higher in the initial half of polyculture period than that in the latter half one, with lower relative abundance of Firmicutes in the early half of polyculture periods. The sequencing also indicated that the enriched Pelagibacter and Pseudoalteromonas enhanced the ability of algae degradation, promoted the resistance to eutrophication caused by feed, and contributed to maintain the environmental conditions for the survival and growth of cultured species. Moreover, the accumulation of Pseudomonas, Acidimicrobiia, and Clostridia in the latter half of polyculture period potentially improved the ammonia and nitrite removal, promoting the water quality, and reduced the infection risk of cultured species.
关晓燕, 王摆, 蒋经伟, 田甲申, 董颖, 周遵春, 王旭达. 仿刺参—中国明对虾—斑节对虾混养下水体微生物组成和多样性[J]. 水产科学, 2020, 39(6): 796-803.
GUAN Xiaoyan, WANG Bai, JIANG Jingwei, TIAN Jiashen, DONG Ying, ZHOU Zunchun, WANG Xuda. Bacterial Community and Diversity Analysis in a Polyculture System of Sea Cucumber Apostichopus japonicus with Chinese Shrimp Fenneropenaeus chinensis, and Tiger Shrimp Penaeus monodon. 水产科学, 2020, 39(6): 796-803.
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2020, Vol. 39 
