Analysis of Genetic Diversity of Little Scale Mandarinfish Coreoperca whiteheadi Population Based on Cytb and D-Loop Genes
YU Ke1, AN Miao1, HUANG Sheng1, PAN Qiuzhi1, NIE Fushun2, HE Xiaokuan3
1. College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Agriculture and Rural Affairs Bureau of Pingtang County, Qiannan 558300, China; 3. Agriculture and Rural Affairs Bureau of Libo, Qiannan 558400, China
Abstract：The genetic diversity, genetic structure and population history of little scale mandarinfish (Coreoperca whiteheadi) were investigated in 86 samples collected in Tongren Jinjiang River in the upper Yuanjiang River, Pingzhou River in Pingtang County and Zhangjiang River in Libo County by mitochondrial DNA Cytb gene and D-Loop gene analysis in order to explore the diversity of wild resources of little scale mandarinfish in Guizhou Province. The sequence was found to be 1141 bp in Cytb and 822 bp in D-Loop in length. In the Cytb region, 11 haplotypes were detected and 18 haplotypes were defined, with mean haplotype diversity of 0.918, mean nucleotide difference of 3.342 and mean nucleotide diversity of 0.00293. A total of 17 mutation sites were detected in the D-Loop region, including 3 deletions and 2 insertions, and 23 haplotypes were found, with mean haplotype diversity of 0.903, mean nucleotide difference of 3.343, and mean nucleotide diversity of 0.00410. The genetic differentiation index indicated that there was significant genetic differentiation among the three populations, and both the neutral test and the nucleotide mismatch analysis revealed that little scale mandarinfish had not experienced population expansion. The findings showed that the population structure of little scale mandarinfish in Guizhou Province was primitive due to inbreeding decline. The habitat of the population in the Yangtze River Basin is ideal, with great evolutionary potential. The population in Libo Zhangjiang River is at risk, and Jinjiang River is more suitable for little scale mandarinfish breeding. In this paper, the genetic diversity of little scale mandarinfish in Guizhou Province was evaluated at molecular level, which provides reference for the protection and utilization of its germplasm resources.
余科, 安苗, 黄胜, 潘秋芝, 聂福顺, 何孝宽. 基于Cytb和D-Loop基因对中国少鳞鳜种群遗传多样性的分析[J]. 水产科学, 2020, 39(6): 894-901.
YU Ke, AN Miao, HUANG Sheng, PAN Qiuzhi, NIE Fushun, HE Xiaokuan. Analysis of Genetic Diversity of Little Scale Mandarinfish Coreoperca whiteheadi Population Based on Cytb and D-Loop Genes. Fisheries Science, 2020, 39(6): 894-901.
[1]宋书莉.鳜类的物种界定、系统发育和生物地理以及少鳞鳜属群体遗传现状和演化过程的研究[D].上海:上海海洋大学,2017. [2]蒋志刚,江建平,王跃招,等.中国脊椎动物红色名录[J].生物多样性,2016,24(5):500-551. [3]赵丽丽,赵金良.中国少鳞鳜不同群体mtDNA控制区序列的遗传变异分析[J].上海水产大学学报,2007,16(5):409-413. [4]赵丽丽,赵金良.中国少鳞鳜不同地理群体遗传变异的AFLP分析[J].农业生物技术学报,2008,16(5):907-908. [5]Cao L, Liang X F, Tang W, et al. Phylogeography of Coreoperca whiteheadi (Perciformes: Coreoperca) in China based on mitochondrial and nuclear gene sequences[J]. Biochemical Systematics and Ecology,2013,50 (Complete):223-231. [6]余科,安苗,黄胜,等.线粒体COⅠ基因在少鳞鳜属(Coreoperca)鱼类条形码区段的变异分析[J/OL].基因组学与应用生物学(2019-04-19)[2020-05-13].https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CAPJ&dbname=CAPJLAST&filename=GXNB20190416002&v=MDIzNTJaK1ptRnkvbFY3ck5JbDA9SWpYRmJMRzRIOWpNcTQ1RFpPc05ZdzlNem1SbjZqNTdUM2ZscVdNMENMTDdSN3Fk. [7]陈宜瑜,曹文宣,郑慈英.珠江的鱼类区系及其动物区划的讨论[J].水生生物学报,1986,10(3):228-236. [8]唐文乔,陈宜瑜,伍汉霖.武陵山区鱼类物种多样性及其动物地理学分析[J].上海水产大学学报,2001,10 (1):6-15. [9]代应贵.贵州都柳江的鱼类区系及动物地理区划[J].水产学报,2011,35(6):871-879. [10]Grant W.Shallow population histories in deep evolutionary lineages of marine fishes: insights from sardines and anchovies and lessons for conservation[J].Journal of Heredity,1998,89(5):415-426. [11]肖武汉,张亚平.鱼类线粒体DNA的遗传与进化[J].水生生物学报,2000,24(4):384-391. [12]陈四海,区又君,李加儿.鱼类线粒体DNA及其研究进展[J].生物技术通报,2011(3):13-20. [13]马春艳,赵峰,孟彦羽,等.基于线粒体细胞色素b基因片段序列变异探讨3种鲳属鱼类系统进化[J].渔业科学进展,2009,30(5):20-26. [14]王伟伟.基于细胞色素b基因序列分析我国鲑亚科鱼类系统发育关系和不同虹鳟养殖群体遗传变异[J].水产科学,2015,34(6):386-390. [15]颉晓勇,李思发.基于细胞色素b序列的罗非鱼选育群体遗传变异分析[J].暨南大学学报:自然科学与医学版,2014,35(5):439-444. [16]李娜,陈少波,谢起浪,等.闽浙地区香鱼线粒体Cytb基因和D-loop区序列多态性分析[J].遗传,2008,30 (7):919-925. [17]孔杰,周洲,刘伟,等.达氏鳇亲鱼群体mtDNA Cytb基因和D-loop区的序列特征及遗传多样性分析[J].江苏农业科学,2019,47(3):40-42. [18]张久盘,刘哲,张波,等.祁连山裸鲤和青海湖裸鲤mtDNA cytb基因和D-loop区序列特征及遗传分化[J].淡水渔业,2015,45(4):92-95. [19]Cao L,Lang X F.A new freshwater perch species of the genus Coreopercaherzenstein(Perciformes,Serranidae,Sinipercinae)from Zhejiang Province,China[J].Acta Zootaxonomica Sinica,2013,38(4):891-894. [20]Lee W J, Conroy J, Howell W H, et al. Structure and evolution of teleost mitochondrial control regions[J]. Journal of Molecular Evolution,1995,41:54-66. [21]牛素芳,苏永全,王军,等.福建近海竹荚鱼线粒体DNA控制区和细胞色素b遗传多态性[J].中国水产科学,2011,18(1):66-74. [22]杨培民,胡宗云,金广海,等.用线粒体D-loop和Cytb基因序列分析拉氏鱼岁3个群体的遗传结构和遗传分化[J].水产学杂志,2017,30(4):9-12. [23]彭士明,施兆鸿,侯俊利.基于线粒体D-loop区与COI基因序列比较分析养殖与野生银鲳群体遗传多样性[J].水产学报,2010,34(1):19-25. [24]Brown W M. The mitochondrial genome of animals[M]//Maclntyre R J.Molecular evolutionary genetics.New York:Plenum Press,1985:95-130. [25]沈朕,关洪斌,郑风荣,等.基于Cytb和D-loop的4个大泷六线鱼群体遗传多样性分析[J].海洋科学进展,2017,35(4):524-534. [26]孙超,刘洪波,姜涛,等.不同鲚属鱼类Cytb和D-loop序列的比较及其判别早期生活史个体的潜力分析[J].渔业科学进展,2018,39(5):27-35. [27]颉晓勇,李思发.罗非鱼选育群体Cytb与D-loop序列变异信息对比分析[J].基因组学与应用生物学,2014,33(5):982-985. [28]陈增祥,刘丽,袁吉贵,等.基于D-loop序列的吉富罗非鱼遗传变异分析[J].海南热带海洋学院学报,2018,25(2):80-83. [29]陈金平,董崇智,孙大江,等.微卫星标记对黑龙江流域大麻哈鱼遗传多样性的研究[J].水生生物学报,2004,28(6):607-612. [30]司从利.珠江水系鳜鱼的遗传多样性研究[D].广州:暨南大学,2012. [31]白晓慧,杨华,孟一耕,等.大鳞副泥鳅群体线粒体DNA D-loop序列遗传变异分析[J].水产科学,2018,37 (5): 658-664. [32]周文漪 基于线粒体细胞色素b的7水系斑鳜遗传多样性分析[D].广州:暨南大学,2014. [33]王伟伟,赵金良,李思发.我国斑鳜不同群体mtDNA控制区序列的遗传变异[J].上海水产大学学报,2006,15(4):398-402. [34]Wright S.Evolution and the genetics of population:variability within and among natural populations[M]. Chicago: University of Chicago Press,1978. [35]Balloux F, Lugon- Moulin N. The estimation of population differentiation with microsatellite markers[J].Molecular Ecology,2002,11(2):155-165. [36]孙鹏,彭士明,尹飞,等.利用COI和Cytb序列探讨东海区3种鲳属鱼类的种群遗传结构[J].海洋渔业,2011,33 (4):398-404. [37]王伟伟,赵金良,李思发.我国斑鳜六个群体mtDNACytb序列的遗传变异[J].动物学研究,2006,27(6):589-593. [38]周启永,陈佩英.贵州及邻区晚新生代岩相古地理演化[J].贵州地质,1993,10(3),201-207.
2020, Vol. 39 
