Nutrient Requirement of Kelp Laminaria hyperborea Seedlings for Nitrogen, Phosphorus and Iron
DU Xinxin1, LIU Fuli2,3, YUAN Yanmin2, LIANG Zhourui2,3, ZHANG Pengyan2,3, LIU Shengping1
1.College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China; 2.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 3.Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China
Abstract：Kelp Laminaria hyperborea seedlings with length of 35 cm were cultivated in filtered and sterilized seawater in aeration at nitrogen and phosphorus concentrations [ρ(N)∶ρ(P)=10∶1] of 0 mg/L, 0.5 mg/L, 2 mg/L, 4 mg/L, 6 mg/L, 8 mg/L and 10 mg/L in NO3--N, with NaNO3 and KH2PO4 as nitrogen and phosphorus sources at water temperature of 9 ℃, and photoperiod of 12L∶12D. After 12 h, 24 h, 36 h, 48 h and 60 h, the chlorophyll fluorescence parameters including photosystem Ⅱ maximum fluorescence yield, and Fv/Fm and the apparent photosynthetic rates of seedlings were determined in 12 h, 24 h and 48 h cultivation. In another trial, the kelp seedlings were cultivated at Fe3+ concentration of 0 mg/L and 1 mg/L in NH4+-N (3 mg/L) groups and 0 mg/L, 0.2 mg/L, 1 mg/L and 5 mg/L NO3--N (3 mg/L) groups, with NaNO3 and NH4Cl as the nitrogen source, KH2PO4 as phosphorus source, and FeCl3 as iron source. Then concentrations of PO43--P, NO3-N, NH4+ -N and Fe3+ were analyzed in the seedling culture medium in 0 h, 4 h, 10 h, 24 h and 48 h and the relative growth rate, maximum fluorescence yield (Fv/Fm) of photosystem and Fe3+ concentration were measured in the kelp seedlings exposed to various concentrations of nitrogen, phosphorus, and Fe3+ in 3 days to explore the optimal nutrient concentrations and to understand the interaction mechanism of the demand of Fe, N and P, the relative growth rate, the maximum quantum yield of (Fv/Fm) and apparent photosynthetic rate of the kelp seedlings under different nutrient concentrations. The results showed that there was significantly higher relative growth rate in the kelp seedlings exposed to 2—4 mg/L of NO3--N, and 0.2—0.4 mg/L of PO43--P, indicating that the concentration ranges of NO3--N and PO43--P was favorable for the seedling growth. The higher Fv/Fm and apparent photosynthetic rate were observed in the seedlings exposed to NO3--N concentration of 2—4 mg/L, and PO43--P concentration of 0.2—0.4 mg/L, indicating that the concentration range of NO3--N and PO43--P was favorable for photosynthesis. The good growth was found in the seedlings exposed to Fe3+ concentration of 0.2—1.0 mg/L, with the maximal relative growth rate at Fe3+ concentration of 1 mg/L. The appropriate iron concentration led to significant improvement of uptake of NO3--N and PO43--P by the seedling within 10 h, without significant effect in 24 h. At the same time, the absorption of iron by seedlings was basically saturated within 10 h, and the absorption of NO3--N and PO43-P was inhibited at iron concentration of 5 mg/L, without significant effect of addition of iron on absorption of NH4+-N (P>0.05).
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2021, Vol. 40 
