Effects of Nitrogen Concentration on Growth and Biochemical Characteristics of Young Kelp Laminaria hyperborea Seedlings
YUAN Yanmin1, LIU Fuli1,2, LIANG Zhourui1,2, WANG Xiao1,3
1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China; 3. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Abstract：Kelp Laminaria hyperborea has great potential for seaweed bed construction and artificial cultivation in China. To clarify the suitable nitrate concentration for the growth of young kelp seedlings and the biochemical responses under nitrogen stress, the relative growth rate and biochemical properties were investigated under different nitrate concentration(0, 0.5, 2, 4, 6, 8, and 10 mg/L). The main results were as follows: (1) the optimum nitrate concentration for growth of young kelp seedling was 2—8 mg/L. The malondialdehyde (MDA) content of young seedlings was higher at low nitrate concentration, and it was negatively interrelated with the relative growth rate (RGR), indicating that the membrane lipid peroxidation may be one of the reasons contributing to the low relative growth rate of young kelp seedlings. (2) At low nitrogen concentration (0—0.5 mg/L), the content of superoxide anion (SA) and the specific activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in young kelp seedlings increased significantly, indicating that these three antioxidant enzymes, especially POD played a key role in action of clearing the free radicals. However, there was a more obvious accumulation of MDA, indicating that the action of antioxidant enzymes may be limited to scavenge the SA. At high nitrogen concentration (10 mg/L), there was no significant accumulation of SA and MDA, and the specific activities of SOD, POD and CAT were increased significantly, indicating that the antioxidant enzyme system of young seedlings can function at high nitrogen concentration and scavenge the accumulated reactive oxygen species. In general, high and low concentrations of nitrate nutrient showed different effects on the antioxidant enzyme system of the young kelp seedlings. (3) In the high and low concentrations of nitrate nutrient and phosphate, carotenoid and chlorophyll-a contents were lower than the appropriate concentration, indicating that it had not respond to nitrogen stress significantly. These findings can provide theoretical guidelines for the seedling, breeding and artificial cultivation of kelp L. hyperborea.
袁艳敏,刘福利,梁洲瑞,王潇. 氮营养盐对极北海带幼苗生长和生理生化特性的影响[J]. 水产科学, 2021, 40(2): 203-209.
YUAN Yanmin, LIU Fuli, LIANG Zhourui, WANG Xiao. Effects of Nitrogen Concentration on Growth and Biochemical Characteristics of Young Kelp Laminaria hyperborea Seedlings. 水产科学, 2021, 40(2): 203-209.
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2021, Vol. 40 
