Karyotype and Cellular DNA Contents of Culter Hybrid F1Erythroculter ilishaeformis (♀) × Ancherythroculter nigrocauda (♂), “Xianfeng No. 1”
WANG Xin1,2, MA Hao1,2, ZHU Dongmei1,2, SUN Ning1,2, LI Qing1, WANG Guiying1, CHEN Jian1, SUN Yanhong1, LI Pei1
1. Wuhan Xianfeng Aquatic Science and Technology Co. , Ltd. , Fisheries Research Institute, Wuhan Academy of Agricultural Science, Wuhan 430207, China; 2. College of Fisheries,Huazhong Agricultural University, Wuhan 430070, China
Abstract：The chromosome number and karyotype were investigated in juvenile culter hybrid F1 [Erythroculter ilishaeformis (♀) × Ancherythroculter nigrocauda (♂)] (known as“Xianfeng No 1”) with body weight of 22—88 g injected with phytohemagglutinin (PHA) at a dose of 6 μg/g, and injected with colchicine at a dose of 8 μg/g after 12—14 h, and 2 h later, blood was sampled via caudal vein, routine air drying, Giemsa staining, photomicrography and Photoshop CS6 Software analysis and other steps. The cellular DNA content of culter hybrid F1, “Xianfeng No 1”, was determined by a flow cytometry via absolute content of DNA from rooster blood cells (2.50 pg/2C) as a control. The results showed that there were 48 chromosomes in culter hybrid F1, with karyotype formula of 28m+18sm+2st, and NF=94, without secondary constriction, satellite, heterosome and sex chromosome. The culter hybrid F1 had DNA content of (3.16±0.04) pg/2C, and the same chromosome number as their parents, with more similar karyotype to the male parent A. nigrocauda. There were no significant differences in the DNA content between the culter hybrid F1 and their parents.
[1]楼允东,李小勤.中国鱼类远缘杂交研究及其在水产养殖上的应用[J].中国水产科学,2006,13(1):151-158. [2]王石,汤陈宸,陶敏,等.鱼类远缘杂交育种技术的建立及应用[J].中国科学:生命科学,2018,48(12):1310-1329. [3]李佩,李清,王贵英,等.氨氮和亚硝态氮对杂交鲌先锋1号的急性毒性试验[J].湖北农业科学,2017,56(14):2728-2730. [4]陈见,李清,王贵英,等.翘嘴红鲌(♀)×黑尾近红鲌(♂)杂种F1的AFLP分析[J]. 水生生物学报,2014,38(5):891-896. [5]湖北武汉杂交鲌“先锋1号”正式获水产新品种证书[J]. 科学养鱼,2013(6):49. [6]李国珍.染色体及其研究方法[M].北京:科学出版社,1985:108-129. [7]吉华松,周永灿,蔡岩,等.六带石斑鱼染色体核型和银染研究[J].水产科学,2011,30(8):463-466. [8]曾庆凯,杜合军,杨菁.鲟形目鱼类染色体研究现状及进展[J].水产科学,2019,38(5):734-740. [9]李学军,王阅雯,高丽霞,等.淇河鲫与野生鲫形态及染色体差异研究[J].水产科学,2010,29(11):674-676. [10]Gouveia J G, de Moraes-Manécolo V P O, Swarca A C, et al. Cytogenetic trends in two families of the neotropical catfishes: Heptapteridae and Pseudopimelodidae (Siluriformes)[J]. Zebrafish,2018,15(6):629-641. [11]Serrano É A, Melo B F, Freitas-Souza D, et al. Species delimitation in neotropical fishes of the genus Characidium (Teleostei, Characiformes)[J]. Zoologica Scripta,2019,48(1):69-80. [12]Conde-Saldaña C C, Cunha M S, Albornóz-Garzon J G, et al. Karyotypic divergence of two co-occurring species of andean climbing catfishes (Siluriformes: Loricarioidei: Astroblepidae) [J]. Zebrafish,2019,16(1):106-114. [13]Liu L, Cui Z X, Song C W, et al. Flow cytometric analysis of DNA content for four commercially important crabs in China [J]. Acta Oceanologica Sinica,2016,35(6):7-11. [14]Zhu D M, Song W, Yang K, et al. Flow cytometric determination of genome size for eight commercially important fish species in China[J]. In Vitro Cellular & Developmental Biology-Animal,2012,48(8):507-517. [15]Xavier P L P, Senhorini J A, Pereira-Santos M, et al. A flow cytometry protocol to estimate DNA content in the yellowtail tetra Astyanax altiparanae[J]. Frontiers in Genetics,2017,8:131. [16]胡文彪,李清,杨瑞斌,等. 翘嘴鲌(♀)和黑尾近红鲌(♂)杂交F1的胚胎发育和胚后发育观察[J].华中农业大学学报,2013,32(1):103-109. [17]吴凡,文华,李清,等.杂交鲌“先锋1号”幼鱼蛋白质需求量的研究[J].动物营养学报,2018,30(12):5052-5061. [18]林义浩.快速获得大量鱼肾细胞中期分裂相的PHA体内注射法[J].水产学报,1982,6(3):201-204. [19]Levan A, Fredga K, Sandberg A A. Nomenclature for centromeric position on chromosomes [J]. Hereditas,2009,52(2):201-220. [20]王晓艳,杨品红,李梦军,等.翘嘴鲌染色体核型分析及遗传学特性研究[J].湖南文理学院学报(自然科学版),2009,21(3):73-77. [21]张伟明,吴萍,吴康,等.两种鱼类染色体制片方法的比较研究[J].水利渔业,2003,23(5):9-10. [22]余先觉,周暾,李渝成,等.中国淡水鱼类染色体[M].北京:科学出版社,1989:23-25. [23]桂建芳,李渝成,李康,等.鰕虎鱼亚目三种鱼染色体组型的比较研究[J].动物学研究,1984,5(3):67-69. [24]易少奎,张新辉,杨坤,等.厚颌鲂核型分析与DNA含量的测定[J].华中农业大学学报,2014,33(1):80-84. [25]刘莉,张岩,陈超,等.棕点石斑鱼(Epinephelus fuscoguttatus♀)×鞍带石斑鱼(Epinephelus lanceolatus♂) F1染色体制备方法及核型分析[J].渔业科学进展,2016,37(2):33-40. [26]葛玲瑞.团头鲂(♀)×翘嘴红鲌(♂)杂交F1的生物学特征及遗传分析[D].长沙:湖南师范大学,2011:1-46. [27]张丽娟,祝斐,尹绍武,等.河川沙塘鳢(♀)×鸭绿沙塘鳢(♂)双亲及其杂交子代的核型分析[J].海洋渔业,2013,35(2):183-188. [28]贺刚,方春林,王伟萍,等.大口鲇(♀)×怀头鲇(♂)杂种F1的形态学及遗传学研究[J].湖北农业科学,2011,50(23):4916-4919. [29]陈友铃,张秋金,汪彦愔,等.彩鲷(♂)×美洲虎鲷(♀)杂交后代及其双亲的染色体研究[J].农业生物技术学报,2009,17(3):469-475. [30]薛淑群,尹洪滨,尹家胜,等.哲罗鱼(Hucho taimen)染色体组型与DNA含量分析[J].广东海洋大学学报,2010,30(3):6-10. [31]叶星,谢刚,祁宝伦,等.广东鲂(♀)×团头鲂(♂)杂交子一代及其双亲染色体组型的分析[J].大连水产学院学报,2002,17(2):102-107. [32]黎玉元.草鱼、赤眼鳟及其杂交F1染色体核型比较分析[D].长沙:湖南农业大学,2014:1-50. [33]林勉,苗亮,李明云,等.真鲷、黑鲷及其杂交子代的染色体构成与AFLP分析[J].海洋学报,2014,36(8):117-121. [34]张佳佳,张国松,张宏叶,等.黄颡鱼(♀)×瓦氏黄颡鱼(♂)双亲及其杂交子代核型和营养成分分析[J]. 海洋渔业,2017,39(2):149-161. [35]成美玲,田永胜,吴玉萍,等.云纹石斑鱼(♀)×鞍带石斑鱼(♂)杂交后代染色体核型分析[J].渔业科学进展,2018,39(2):67-75. [36]方礼豹,周小云,崔蕾,等.二倍体、四倍体泥鳅与大鳞副泥鳅杂交子代DNA相对含量与染色体组型的比较[J].华中农业大学学报,2011,30(4):500-505. [37]何伟国.红鲫与翘嘴红鲌不同倍性水平杂交鱼的形成及其生物学特征研究[D].长沙: 湖南师范大学,2012:1-137. [38]曹丽萍,丁炜东,贾永义,等.奥利亚罗非鱼(♀)×鳜(♂)杂交后代及其双亲的染色体核型分析[J]. 农业生物技术学报,2006,14(2):187-190. [39]杨坤,王子健,祝东梅,等.麦穗鱼的组型分析和DNA含量测定[J].华中农业大学学报,2012,31(3):371-375. [40]邹远超,袁佳玲,符鹏,等.宽体沙鳅的染色体核型与DNA含量分析[J].动物学杂志,2017,52(3):441-448. [41]郑国栋,张倩倩,李福贵,等.团头鲂(♀)×翘嘴鲌(♂)杂交后代的遗传特征及生长差异[J].中国水产科学,2015,22(3):402-409. [42]乔庆,刘巧林,肖调义,等. 草鱼(♀)×赤眼鳟(♂)杂交F1倍性分析和性腺发育特点[J].水生生物学报,2018,42(2):313-322. [43]刘阳阳.小黄鱼与大黄鱼的种间杂交及F1代鉴定[D].舟山:浙江海洋大学,2018:1-50.
2021, Vol. 40 
