Inhibition of Black Tea and Green Tea Treated by Different Methods to Alga Alexandrium tamarense
CHEN Xuehao1,2, WU Yuqiang1, YANG Zhi1, ZHOU Lihong1,2
1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Eel Engineering Research Centreof the Modern Industry Technology, Ministry of Education, Xiamen 361021, China
Abstract：Harmful algae bloom will threaten the balance of marine ecosystem, and it is of great significance to find algal inhibiting materials to control red tide. The short-term and long-term inhibitory effects of black tea and green tea on toxic algae Alexandrium tamarense were studied by reasonable application methods including comparing the algal inhibition effect of dry tea powder, water extract and tea residue. The results showed that the 5 h inhibition rates (IRs) of 1 mg/mL tea extract were very high [(99.45±0.39)% (green tea) and (100.00±0.00)% (black tea)], and 1h-IC50 of tea extracts were 0.85 mg/mL green tea and 0.87 mg/mL black tea, respectively. Green tea residues had stronger algal inhibition than black tea residues did, and the lower the concentration was, the greater the difference of the algal inhibition was; the maximal IRs of 0.80 mg/mL green tea and black tea residue were (94.56±1.03)% and (22.17±1.66)%, respectively; 1h-IC50 of green tea and black tea residues were 3.39 mg/mL and 8.96 mg/mL, respectively. The high algal inhibition rate (green tea powder≥(94.23±0.64)%, black tea powder ≥(88.31±2.35)%) of 1 mg/mL tea powder was kept from 24 to 120 hours, and the IR of green tea powder was higher than that of black tea powder before 24 hours, without significant difference between them after 48 hours. In terms of the 72-hour average IR for each concentration, the average IR of green tea extract was 1.95—3.72 times as that of black tea extracts at a concentrations of 0.63 mg/mL or less; the average IR of green tea residue was 1.19—4.24 times as that of black tea residue at concentration between 0.80 mg/mL and 8.00 mg/mL. It was found that green tea had stronger inhibitory effect on A. tamarense than black tea did, and green tea residue was a promising algal inhibiting material.
陈学豪, 武玉强, 杨志, 周立红. 不同处理的红茶与绿茶对塔玛亚历山大藻的抑制作用[J]. 水产科学, 2021, 40(4): 544-552.
CHEN Xuehao, WU Yuqiang, YANG Zhi, ZHOU Lihong. Inhibition of Black Tea and Green Tea Treated by Different Methods to Alga Alexandrium tamarense. Fisheries Science, 2021, 40(4): 544-552.
[1]刘璐,张树林,张达娟,等.小檗碱复合物和微生态制剂控制池塘有害蓝藻[J].水产科学,2017,36(4):443-448. [2]汪瑾,杜明勇,于玉凤,等.几种植物浸提液对铜绿微囊藻的抑制作用及抑藻特性[J].南京农业大学学报,2014,37(4):91-96. [3]Welch I M, Barrett P R F, Gibson M T, et al. Barley straw as an inhibitor of algal growth Ⅰ:studies in the Chesterfield Canal[J].Journal of Applied Phycology,1990,2(3):231-239. [4]Barrett P R F, Littlejohn J W, Curnow J. Long-term algal control in a reservoir using barley straw[M]//Caffrey J, Barrett P R F, Teresa Ferreira M, et al. Biology, ecology and management of aquatic plants. Dordrecht:Springer Netherlands,1999:309-313. [5]Greca M D, Ferrara M, Fiorentino A, et al. Antialgal compounds from Zantedeschia aethiopica[J].Phytochemistry,1998,49(5):1299-1304. [6]姜丹,黄凌风,张可,等.滩涂盐生植物盐角草对东海原甲藻生长的抑制效应[J].湿地科学与管理,2014,10(1):42-47. [7]白羽,黄莹莹,孔海南,等.加拿大一枝黄花化感抑藻效应的初步研究[J].生态环境学报,2012,21(7):1296-1303. [8]高洁,杨维东,刘洁生,等.利用小麦秸控制赤潮生物生长的研究[J].海洋环境科学,2005,24(1):5-8. [9]张卫明,廖日红,许志兰,等.5种天然植物对铜绿微囊藻的抑制效果研究[J].北京水务,2011(5):18-21. [10]Park M H, Han M S, Ahn C Y, et al. Growth inhibition of bloom-forming cyanobacterium Microcystis aeruginosa by rice straw extract[J].Letters in Applied Microbiology,2006,43(3):307-312. [11]张彬,罗本福,梅自良.禾本科植物抑制三峡库区水华优势蓝藻的探讨[J].重庆三峡学院学报,2014,30(3):10-14. [12]孙志伟,田斐,安民,等.红树植物秋茄叶片水提物的抑藻效应[J].生态学杂志,2012,31(9):2241-2246. [13]Li F M, Hu H Y. Isolation and characterization of a novel antialgal allelochemical from Phragmites communis[J].Applied and Environmental Microbiology,2005,71(11):6545-6553. [14]杨维东,张信连,刘洁生,等.杉木粉对塔玛亚历山大藻的抑制作用(英文)[J]. 水生生物学报,2005,29(2):215-218. [15]张信连,杨维东,刘洁生,等.杉木粉对塔玛亚历山大藻生长的影响[J].海洋环境科学,2005,24(2):23-25. [16]杨维东,刘玉荣,刘洁生,等.桉木粉对塔玛亚历山大藻的抑制作用及其化学基础研究[J].环境科学,2007,8 (9):2296-2301. [17]Zhou L H, Zheng T L, Chen X H, et al. The inhibitory effects of garlic (Allium sativum) and diallyl trisulfide on Alexandrium tamarense and other harmful algal species[J].Journal of Applied Phycology,2008,20(4):349-358. [18]张孝杰,陈学豪,周立红.三种材料的抑藻效果及其毒性研究[J].海洋环境科学,2013,32(4):555-559. [19]Zhou L H, Zheng T L, Wang X, et al. Effect of five Chinese traditional medicines on the biological activity of a red-tide causing alga—Alexandrium tamarense[J].Harmful Algae,2007,6(3):354-360. [20]缪莉,董昆明,李楠,等.3种陆生植物化感物质提取液的抑藻活性研究[J].安徽农业科学,2010,38(35):20246-20249,20259. [21]成永旭.生物饵料培养学[M].2版.北京:中国农业出版社,2005:79. [22]赵楠,高慧媛,孙博航,等.茶叶的化学成分[J].沈阳药科大学学报,2007,24(4):211-214. [23]杜继煜,白岚,白宝璋.茶叶的主要化学成分[J].农业与技术,2003,23(1):53-55. [24]王丽,林昕,汪禄祥,等.茶叶中多种功能成分含量测定与分析研究[J].食品安全质量检测学报,2019,10(22):7779-7786. [25]杨延.茶叶中活性成分分析[J].广东蚕业,2019,53(2):22-23. [26]黄浩,余鹏辉,赵熙,等.湖南不同产地工夫红茶品质成分分析[J].茶叶通讯,2019,46(2):208-214. [27]凌彩金,范杰文,王秋霜,等.英红九号红茶生化成分指纹图谱初探[J].广东农业科学,2018,45(12):95-100. [28]郞明洋,付长华.浙江省不同产地茶叶主要成分含量比较及分析[J].机电信息,2018(26):38-44. [29]张威,吴海江,马桂岑,等.海南农垦红茶、绿茶品质分析研究[J].中国茶叶,2018,40(7):25-31. [30]王伟伟,施莉婷,苏威,等.不同嫩度鲜叶加工工夫红茶对品质和内含成分的影响[J].食品科技,2018,43(9):128-133. [31]李丹,曹永,王华,等.茶叶提取物体外抗氧化活性与其功能性成分含量的相关性研究[J].食品与机械,2018,34(6):163-168. [32]李大祥,方世辉,杨荣俊,等.绿茶、红茶加工工艺对茶鲜叶中多酚类物质的影响[J].中国茶叶加工,2005(4):23-29. [33]Yang C S, Maliakal P, Meng X F. Inhibition of carcinogenesis by tea[J].Annual Review of Pharmacology and Toxicology,2002,42(1):25-54. [34]陈挺强,刘淑敏,黄惠华.绿茶与红茶浸提液功能性成分含量和抗氧化能力的差异研究[J].现代食品科技,2014,30(10):141-146. [35]胡迎芬,杭瑚.饮茶方式对茶汤有效成分含量的影响[J].食品工业科技,2002,23(2):27-29. [36]文军,龚志华,杨伟丽,等. 绿茶浸提动态的研究[J].湖南农业大学学报,1999,25(4):293-294. [37]茶道.一杯茶从早泡到晚不可取[J].新农村,2006(6):23.
2021, Vol. 40 
