Abstract：In order to identify the pathogen causing white-nodules disease in internal organs of large yellow croaker Pseudosciaena crocea in sea cages in Ningde and its preliminary pathogenicity, we first took metagenomic analysis to identify the pathogen as Pseudomonas sp.. On the completion of isolation of the pathogenic strains on selective plates, one of the potential pathogenic strains, termed as ND2018, was used for artificial infection. The results confirmed that ND2018 was the pathogen causing white-nodules disease in internal organs, and that the strain was eventually identified as P. plecoglossicida by whole-genome analysis. P. plecoglossicida, as a conditional pathogen, caused the pathogenicity to large yellow croaker as its concentration reached above 1×104 cfu/mL. The histopathological observation showed that cell disintegration and deposited hemosiderin occurred in liver, spleen and kidney of diseased large yellow croaker. Inflamed tissue formed visible white nodules with ND2018 in large yellow croaker. In addition, susceptibility test showed that ND2018 was sensitive to neomycin, kanamycins, furazolidone, ciprofloxacin, compound sulphonamids and polymyxin B, but resistant to enrofloxacin, gentamicin, florfenicol and 20 other antibiotics. The findings provide theoretical data for the healthy breeding, prevention and treatment of large yellow croaker farmed sea cages.
徐春霞. 网箱养殖大黄鱼内脏白点病病原菌分离鉴定及致病性研究[J]. 水产科学, 2021, 40(5): 670-678.
XU Chunxia. Isolation, Identification and Pathogenicity of White-Nodules Disease in Internal Organs of Large Yellow Croaker Pseudosciaena crocea in a Sea Cage. 水产科学, 2021, 40(5): 670-678.
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2021, Vol. 40 
