Embryonic Development and Expression Level of hsp70 Gene of Grass Carp Ctenopharyngodon idella under Different Temperatures
QIU Yuming, LIN Wang, GUO Honghui, ZHANG Ce, LI Li, LI Dapeng
National Experimental Teaching Demonstration Center of Aquaculture, Hubei Provincial Engineering Laboratory of Pond Healthy Aquaculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
Abstract：In order to explore the effect and mechanism of water temperature on embryonic development, the embryos of grass carp Ctenopharyngodon idella obtained by artificial insemination were incubated at different temperatures (22 ℃, 25 ℃, 28 ℃, and 31 ℃) in the present study. The incubation duration, hatching rate and deformity rate as well as the effective accumulative temperature were recorded and calculated along with the body length of the newly hatched larvae and the expression levels of hsp70 gene in the newly hatched larvae were also detected by using real-time quantitative PCR. The results showed that the hatching rate of embryos was increased significantly with increasing exposure temperature between 22—28 ℃, while the deformity rate significantly was decreased. On the contrary, the hatching rate was significantly decreased [(31.51±1.84)%] (P<0.05) and the deformity rate was increased significantly [(90.00±5.79)%] (P<0.05) at 31 ℃. The regression equation between temperature and hatching rate was described as y=-0.0192x2＋0.9695x-11.246 (r2=0.872), and the regression equation between temperature and deformity rate was expressed as y=0.0271x2-1.3608x＋17.035 (r2=0.931). Also, the hatching duration was significantly decreased in a time-dependent way and the effective accumulative temperature was significantly elevated between 22—28 ℃. The value of Q10 was changed from 1.77 to 2.27. In the newly hatched larvae, the body length was increased within the range of 22—28 ℃ but decreased at 31 ℃, and the regression equation between temperature and body length was expressed as y=－0.0357x2＋1.9394x－21.138 (r2=0.999). The relative mRNA expression for hsp70 gene was higher at 28 ℃ than that in other groups (P<0.05). The findings indicated that increasing temperature promoted the activation of heat shock protein in grass carp embryos to some extent, which contributed to regulate homeostasis and promote embryonic development, with the range of optimal temperature of embryonic development of 25—28 ℃ for grass carp.
邱裕明, 林旺, 郭红会, 张策, 李莉, 李大鹏. 不同温度作用下草鱼胚胎的发育及hsp70基因表达[J]. 水产科学, 2021, 40(6): 870-876.
QIU Yuming, LIN Wang, GUO Honghui, ZHANG Ce, LI Li, LI Dapeng. Embryonic Development and Expression Level of hsp70 Gene of Grass Carp Ctenopharyngodon idella under Different Temperatures. 水产科学, 2021, 40(6): 870-876.
[1]农业农村部渔业渔政管理局. 2019中国渔业统计年鉴[M]. 北京:中国农业出版社,2019:25. [2]薛凌展.温度对异育银鲫“中科3号”胚胎发育的影响[J].福建师范大学学报(自然科学版),2014,30(4):76-83. [3]于欢欢,李炎璐,陈超,等. 温度对云纹石斑鱼(Epinephelus moara)(♀)×七带石斑鱼(Epinephelus septemfasciatus)(♂)杂交F1胚胎发育和仔鱼活力的影响[J]. 渔业科学进展,2014,35(5):109-114. [4]周鑫,董云伟,王芳,等.草鱼hsp70和hsp90对温度急性变化的响应[J].水产学报,2013,37(2):216-221. [5]钟全福.黑莓鲈胚胎发育观察及温度对胚胎发育的影响[J].福建水产,2014,36(5):333-343. [6]吴利敏,袁淑仪,赵晨华,等. 不同温度对淇河鲫胚胎发育的影响[J].山西农业大学学报(自然科学版),2018,38(7):50-56. [7]周明华,余祥胜,强晓刚,等.温度对乌苏里拟鲿受精孵化及仔鱼生长的影响[J].水产养殖,2019,40(10):39-43. [8]薛家骅. 几种理化因子对草鱼胚胎发育的影响[J]. 动物学杂志,1980(3):4-7. [9]郭永灿.水温对鲢鱼、草鱼胚胎发育的影响[J].淡水渔业,1982,12(3):35-40. [10]Lan C, Sun X N, Zhou X C, et al. Preinduced intestinal HSP70 improves visceral hypersensitivity and abnormal intestinal motility in PI-IBS mouse model[J].Asian Pacific Journal of Tropical Medicine,2016,9(3):302-305. [11]陈明帅,徐超,宋兴超,等.热休克蛋白的研究进展[J].经济动物学报,2016,20(1):44-53. [12]陈张帆,宋莉妮,王鹏飞,等.牙鲆胚胎低温处理下内参基因筛选及CIRP和Hsp70基因表达分析[J].农业生物技术学报,2018,26(8):1382-1391. [13]Manos-Turvey A, Brodsky J L, Wipf P. The effect of structure and mechanism of the Hsp70 chaperone on the ability to identify chemical modulators and therapeutics[M]//McAlpine S R, Edkins A L.Heat shock protein inhibition. Switzerland: Springer,Cham,2015:81-129 [14]Horowitz S, Koldewey P, Stull F, et al. Folding while bound to chaperones[J].Current Opinion in Structural Biology,2018,48:1-5. [15]强俊,杨弘,王辉,等.急性温度应激对吉富品系尼罗罗非鱼(Oreochromis niloticus)幼鱼生化指标和肝脏HSP70 mRNA表达的影响[J].海洋与湖沼,2012,43(5):943-953. [16]李筝.尖头大吻鱥HSPs基因克隆及热应激下HSPs和免疫相关基因的表达响应[D]. 新乡:河南师范大学,2018. [17]田照辉,徐绍刚,王巍,等.急性热应激对西伯利亚鲟HSP70 mRNA表达、血清皮质醇和非特异性免疫的影响[J].水生生物学报,2013,37(2):344-350. [18]樊欣,彭仁.热休克蛋白70:生物学功能与作用机制研究进展[J].生命科学,2019,31(3):270-278. [19]姜礼燔,曹萃禾.热污染对草鱼、鲢鱼胚胎发育的影响[J]. 中国环境科学,1984,4(6):40-45. [20]刘艳超,刘海平,刘书蕴,等.温度对尖裸鲤胚胎发育及其仔稚鱼生长性状的影响[J]. 动物学杂志,2018,53(6):910-923. [21]王亚利,解崇友,尹敏,等.温度对四川华鳊胚胎发育的影响[J].水产科学,2020,39(1):79-87. [22]李青,陈永祥.温度对虾蟹生长发育影响的研究进展[J].江苏农业科学,2019,47(10):26-31. [23]张彬,韦嫔媛,熊建华,等.凡纳滨对虾胚胎发育进程及诱导染色体加倍时间确定[J].南方农业学报,2014,45(4):664-670. [24]王国恩.鱼类早期胚胎发生及环境影响[J].东北师大学报(自然科学版),1988(3):120-136. [25]李霞.水产动物组织胚胎学[M].北京:中国农业出版社,2005:243-244. [26]马燕武,张人铭,吐尔逊,等.温度和水流对扁吻鱼胚胎发育的影响[J].淡水渔业,2008,38(1):57-61. [27]陈凤梅,胡家会,王曰文,等.温度对玫瑰无须鲃胚胎发育的影响[J]. 淡水渔业,2013,43(1):24-27. [28]胡振禧,黄洪贵,吴妹英,等.温度对斑鳜胚胎发育的影响[J]. 淡水渔业,2014,44(3):104-107. [29]Budina-Kolomets A, Webster M R, Leu J I J, et al. HSP70 inhibition limits FAK-dependent invasion and enhances the response to melanoma treatment with BRAF inhibitors[J].Cancer Research,2016,76(9):2720-2730. [30]Liu J, Liu J, Guo S Y, et al. HSP70 inhibitor combined with cisplatin suppresses the cervical cancer proliferation in vitro and transplanted tumor growth:an experimental study[J].Asian Pacific Journal of Tropical Medicine,2017,10(2):184-188. [31]Coto A L S, Seraphim T V, Batista F A H, et al. Structural and functional studies of the Leishmania braziliensis SGT co-chaperone indicate that it shares structural features with HIP and can interact with both Hsp90 and Hsp70 with similar affinities[J].International Journal of Biological Macromolecules,2018,118:693-706. [32]Givisiez P E N, Furlan R L, Malheiros E B, et al. Incubation and rearing temperature effects on Hsp70 levels and heat stress response in broilers[J].Canadian Journal of Animal Science,2003,83(2):213-220. [33]Banh A, Vijayan M M, Sivak J G. Hsp70 in bovine lenses during temperature stress[J].Molecular Vision,2003,9:323-328. [34]Dang W, Xu N, Zhang W, et al. Differential regulation of Hsp70 expression in six lizard species under normal and high environmental temperatures[J]. Pakistan Journal of Zoology,2018,50(3):1043-1051. [35]Lewis M, Götting M, Anttila K, et al. Different relationship between hsp70 mRNA and hsp70 levels in the heat shock response of two salmonids with dissimilar temperature preference[J].Frontiers in Physiology,2016,7:511. [36]Mottola G, Nikinmaa M, Anttila K. Hsp70s transcription-translation relationship depends on the heat shock temperature in zebrafish[J].Comparative Biochemistry and Physiology Part A,Molecular & Integrative Physiology,2020,240:110629. [37]Hallare A V, Schirling M, Luckenbach T, et al. Combined effects of temperature and cadmium on developmental parameters and biomarker responses in zebrafish (Danio rerio) embryos[J].Journal of Thermal Biology,2005,30(1):7-17. [38]刘丽丽,王晓雯,朱建亚,等.虎皮鱼热激蛋白PtHsp70基因序列与低温表达分析[J].水产科学,2019,38(6):774-782. [39]Cardoso C M, Sartorio P V, Machado A S D, et al. Hsp70 and p53 expressions and behavior of juvenile pompano, Trachinotus carolinus (Perciformes, Carangidae), at controlled temperature increase[J].Journal of Experimental Marine Biology and Ecology,2015,470:34-42. [40]杨杰青,沈新强,蒋玫,等. 盐度、pH对文蛤肌肉、鳃、外套膜HSP70基因表达的影响[J].水产科学,2016,35(4):398-403. [41]Gray C C, Amrani M, Yacoub M H. Heat stress proteins and myocardial protection:experimental model or potential clinical tool?[J].The International Journal of Biochemistry & Cell Biology,1999,31(5):559-573.
2021, Vol. 40 
