Effects of Different Lipid Levels Diet Supplemented with Surfactin in Orange Spotted Grouper Epinephelus coioides
ZHU Huaining1, SUN Xiuwen1, ZHAI Shaowei1,2
1. Fishery College of Jimei University, Xiamen 361021, China; 2. Eel Engineering Research Center of the Modern Industry Technology, Ministry of Education, Xiamen 361021, China
Abstract：The 56 days feeding trail was conducted to investigate the effects of different lipid levels diet supplemented with surfactin in orange spotted grouper Epinephelus coioides. A total of 480 healthy juvenile grouper with the mean body weight of (23.36±0.06) g were randomly assigned to six treatments with four replicates per treatment and 20 fish per replicate. Six experimental diets were formulated to contain three lipid levels (6%,10% and 14%) and two surfactin supplemental levels (0 mg/kg and 100 mg/kg). Compared with 6% lipid group and 10% lipid group without surfactin supplementation, the weight gain rate and specific growth rate of those two groups with surfactin supplementation were significantly improved (P<0.05), and similar with the parameters in the 14% lipid group without surfactin supplementation (P>0.05); the feed conversion ratio and food intake were significantly decreased (P<0.05). When the dietary lipid level was 14%, the above growth performance parameters were not significantly affected by surfactin supplementation (P>0.05). The intestinal lipase activity were significantly increased by surfactin supplementation at different lipid levels (P<0.05), the activities of amylase and protease were not significantly affected (P>0.05). The content of total cholesterol were decreased significantly (P<0.05), the content of high-density lipoprotein cholesterol increased significantly (P<0.05), and the level of low-density lipoprotein cholesterol were not significantly affected by the surfactin supplementation (P>0.05), while the level of triglyceride was decreased significantly by surfactin supplementation at 10% and 14% lipid levels (P<0.05). In conclusion, dietary surfactin supplementation improved the activity of lipase in intestine, blood lipid level and growth of juvenile grouper at 6% and 10% lipid levels, and the growth performance were not significantly affected at 14% lipid level.
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2021, Vol. 40 
