Effects of Temperature on Growth and Development of Gametophytes in Seaweed Kjellmaniella crassifolia
CHEN Shuxiu, LI Xia, ZHAO Nan, SUN Juan, SHI Liang, LI Xiaojie
Key Laboratory of Algae Genetic Breeding and Cultivation Technology in Shandong Province, National Alga and Sea Cucumber Engineering Technology Research Center, Shandong Oriental Ocean Group Co., Ltd., Yantai 264003, China
Abstract：Ten mL of suspension fluid containing female and male gametophytes of seaweed Kjellmaniella crassifolia with 2♀∶1♂ ratio were cultivated in a petri dish with a diameter of 12 cm at water temperature of 5, 8, 12, 15, 20, and 25 ℃, photoperiod of 10L∶14D, and light intensity of 1000 lx to explore the effect of water temperature on the gametophyte growth and development via determination of germination rate including oocystogenesis rate, ovulation rate, and seedling formation rate. It was found that 80%—90% of the gametophytes was developed into juveniles at 8—12 ℃, with 50% of the seedlings. At water temperature of 5 ℃ and 15 ℃, however, 70%—80% of the gametophytes were still kept at the vegetative stage, although a small proportion of gametophytes (20%—30%) entered into the developmental state, significantly retared behind the group teated at 8—12 ℃. Those gametophytes were all remained at vegetative stage at 20 ℃ and 25 ℃. It is concluded that 8—12 ℃ is the optimum condition for the development of gametophytes.
陈书秀, 李霞, 赵楠, 孙娟, 史良, 李晓捷. 温度对笼目海带配子体生长发育的影响[J]. 水产科学, 2021, 40(6): 895-899.
CHEN Shuxiu, LI Xia, ZHAO Nan, SUN Juan, SHI Liang, LI Xiaojie. Effects of Temperature on Growth and Development of Gametophytes in Seaweed Kjellmaniella crassifolia. 水产科学, 2021, 40(6): 895-899.
[1]吴建东,于广利,李苗苗,等.厚叶切氏海带多糖的提取分离及其结构表征[J].中国海洋大学学报(自然科学版),2011,41(7/8):127-130. [2]王鸿,张甲生,严银春,等.褐藻岩藻多糖生物活性研究进展[J].浙江工业大学学报,2018,46(2):209-215. [3]孙蓓蓓,韩龙江,潘玉龙,等.温度胁迫对海带孢子体生长的影响[J].现代农业科技,2018(20):177-178. [4]朱明远,吴荣军,李瑞香,等.温度对海带幼孢子体生长和光合作用的影响[J].生态学报,2004,24(1):22-27. [5]梁洲瑞,刘福利,杜欣欣,等.光强对极北海带幼苗生长和生化特性的影响[J].渔业科学进展,2019,40(4):115-122. [6]Bartsch I, Vogt J, Pehlke C, et al. Prevailing sea surface temperatures inhibit summer reproduction of the kelp Laminaria digitata at Helgoland (North Sea)[J].Journal of Phycology,2013,49(6):1061-1073. [7]Ratcliff J J, Soler-Vila A, Hanniffy D, et al. Optimisation of kelp (Laminaria digitata) gametophyte growth and gametogenesis:effects of photoperiod and culture media[J].Journal of Applied Phycology,2017,29(4):1957-1966. [8]Müller R, Wiencke C, Bischof K. Interactive effects of UV radiation and temperature on microstages of Laminariales (Phaeophyceae) from the Arctic and North Sea[J].Climate Research,2008,37(2/3):203-213. [9]曹增梅,王伟伟,曲艳艳,等.海带“东方7号”氨基酸季节变化分析与评价[J].水产科学,2017,36(2):132-137. [10]王潇,刘福利,梁洲瑞,等.铁离子对海带配子体克隆系生长发育的影响[J] 中国水产科学,2020,27(9):1052-1061. [11]刘世禄.水产养殖苗种培育技术手册[M].北京:中国农业出版社,2000:177-198. [12]方宗熙,欧毓麟,崔竞进,等.海带配子体无性生殖系培育成功[J].科学通报,1978,23(2):115-116. [13]杨官品,李晓捷,丛义周,等.海带配子体无性繁殖(克隆)技术研究与应用进展[J].中国海洋大学学报(自然科学版),2007,37(4):569-572. [14]Li X J, Liu J L, Cong Y Z, et al. Breeding and trial cultivation of Dongfang No. 3, a hybrid of Laminaria gametophyte clones with a more than intraspecific but less than interspecific relationship[J].Aquaculture,2008,280(1/2/3/4):76-80. [15]曾呈奎,吴超元,任国忠.温度对海带配子体生长发育的影响[J].海洋与湖沼,1962,4(1/2):22-28. [16]李晓捷,王国文,张全胜,等.温度对“901”海带配子体克隆生长的影响[J].水产科技情报,2004,31(4):166-168. [17]孙娟,潘金华,张壮志,等.温度和光照强度对多肋藻(Costaria costata)配子体生长影响研究[J].渔业研究,2019,41(1):11-17. [18]王军,张泽宇,张晓东.温度与照度对利尻海带配子体及幼孢子体的影响[J].中国水产,1999(2):39-41. [19]Martins N, Tanttu H, Pearson G A, et al. Interactions of daylength, temperature and nutrients affect thresholds for life stage transitions in the kelp Laminaria digitata (Phaeophyceae)[J].Botanica Marina,2017,60(2):109-121. [20]Schreiber E. Untersuchungen über parthenogenesis, geschlechtsbestimmung und bastardierungsvermögen Bei laminarien[J].Planta,1930,12(3):331-353.
2021, Vol. 40 
