Molecular Cloning, Identification and Expression Analysis of Progesterone Receptor Gene in Spotted Scat Scatophagus argus
SHI Hongjuan1,2,3, HUANG Jianxuan1, ZHU Chenyang1, WU Jiagang1, HE Feixiang1, JIANG Dongneng1,2,3, HUANG Yang1,2,3, ZHU Chunhua1,2,3, LI Guangli1,2,3
1. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; 2. Guangdong Province Famous Fish Reproduction Regulation and Breeding of Engineering Technology Research Center, Zhanjiang 524088, China; 3. The Key Laboratory of Marine Ecology and Aquaculture Environment in Zhanjiang, Zhanjiang 524088, China
Abstract：Progesterone receptor (Pgr) encoded by Nr3c3 belongs to the subfamily 3 group C of nuclear receptors. Progesterone was bind to its special target receptor Pgr to regulation its roles in production in vertebrates. In this study, progesterone receptor gene (pgr gene) of spotted scat Scatophagus argus was cloned. The RT-PCR and Real-time PCR were performed to explored the tissues distribution and expression of pgr mRNA in gonads at different stages, respectively. Comparison of pgr homology using Megalign software and construction of the phylogenetic tree using MEGA 7.0.4 software was performed in spotted scat. The pgr cDNA was identified and cloned. It was found that open reading frame (ORF) of pgr was 2016 bp, encoded 671 amino acids. Homologous sequence alignment analysis showed that pgr in large yellow croaker Larimichthys crocea displayed the higher similarity to that in spotted scat compared to other fish. RT-PCR analysis showed that pgr RNA was expressed in all tissues, with high expression in gonad and pituitary in which pgr mRNA expression was significantly up-regulated by exogenous E2 (17β-Estrodiol). Furthermore, the expression of gonadal pgr mRNA was higher at maturation stage than in other developmental stage, which indicates that pgr plays an important role in regulation of the production of the matured eggs and sperm.
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2022, Vol. 41 
