Abstract：In this study, we predict the secretory proteins of microsporidium Hepatospora eriocheir genome parasited in Chinese mitten crab Eriocheir sinensis with the EuSecPred 2.0 and annotate for secreted protein function, characteristics and the sequence analysis. The length of secreted proteins was found to be primarily changed from 30 amino acids to 300 amino acids, with length of signal peptide from 11 amino acids to 23 amino acids. The signal peptide cleavage site was mainly composed of hydrophobic amino acids. Many key proteins were found by functional annotation of the secretory proteins such as haemaglutinin-esterase, methyltransferase EZH1, and serine/threonine protein kinase. Motif analysis revealed that there was a S[NS]N[TI][FI]VI[IGD][RG]VRC motif in signal peptides which predicted secreted proteins were involved in the physiological activities of H. eriocheir, host cell cycle regulation and immune response. The findings provide important basis for screening disease-related candidate factors and exploring the infection mechanism.
宁梓健, 寇诗瑀, 包杰, 姜宏波. 中华绒螯蟹微孢子虫全基因组分泌蛋白的预测分析[J]. 水产科学, 2022, 41(2): 280-288.
NING Zijian, KOU Shiyu, BAO Jie, JIANG Hongbo. Genome-Wide Prediction and Analysis of Secretory Proteins of Microsporidium Hepatospora eriocheir Parasited in Chinese Mitten Crab Eriocheir sinensis. 水产科学, 2022, 41(2): 280-288.
[1] 胡清彪,李晓东,姜玉声,等.不同家系中华绒螯蟹幼蟹在室外和室内的生长[J].水产科学,2016,35(5):547-551. [2] 赵彦华,刘洪岩,丁正峰,等.江苏省河蟹主养区微孢子虫感染情况调查报告[J].水产养殖,2017,38(1):11-14. [3] DING Z, MENG Q, LIU H, et al. First case of hepatopancreatic necrosis disease in pond-reared Chinese mitten crab, Eriocheir sinensis, associated with microsporidian[J].Journal of Fish Diseases,2016,39(9):1043-1051. [4] 刘慧.中华绒螯蟹微孢子虫的鉴定及流行病学调查[D].南京:南京师范大学,2018. [5] WANG W, GU Z F. Rickettsia-like organism associated with tremor disease and mortality of the Chinese mitten crab Eriocheir sinensis[J].Diseases of Aquatic Organisms,2002,48(2):149-153. [6] 刘洪岩,赵彦华,孙梦玲,等.中华绒螯蟹微孢子虫研究进展[J].水产养殖,2017,38(10):42-47. [7] STENTIFORD G D, BATEMAN K S, DUBUFFET A, et al. Hepatospora eriocheir (Wang and Chen, 2007) gen.et comb.nov.infecting invasive Chinese mitten crabs (Eriocheir sinensis) in Europe[J].Journal of Invertebrate Pathology,2011,108(3):156-166. [8] 陈继圣,郑士琴,郑武,等.全基因组预测稻瘟菌的分泌蛋白[J].中国农业科学,2006,39(12):2474-2482. [9] KAMOUN S, VAN WEST P, VLEESHOUWERS V G, et al. Resistance of Nicotiana benthamiana to Phytophthora infestans is mediated by the recognition of the elicitor protein INF1[J].The Plant Cell,1998,10(9):1413-1426. [10] D′SILVA I, HEATH M C. Purification and characterization of two novel hypersensitive response-inducing specific elicitors produced by the cowpea rust fungus[J].The Journal of Biological Chemistry,1997,272(7):3924-3927. [11] 李田,刘显林,韩冰,等.家蚕微孢子虫全基因组分泌蛋白的预测分析[J].蚕业科学,2013,39(2):295-301. [12] 李田,齐晓冉,陶美林,等.4种微孢子虫的分泌蛋白的比较基因组学分析[J].蚕业科学,2013,39(3):527-536. [13] KROGH A, LARSSON B, von HEIJNE G, et al. Predicting transmembrane protein topology with a hidden Markov model:application to complete genomes[J].Journal of Molecular Biology,2001,305(3):567-580. [14] NGUYEN BA A N, POGOUTSE A, PROVART N, et al. NLStradamus:a simple Hidden Markov Model for nuclear localization signal prediction[J].BMC Bioinformatics,2009,10(1):202. [15] CLAROS M G, VINCENS P. Computational method to predict mitochondrially imported proteins and their targeting sequences[J].European Journal of Biochemistry,1996,241(3):779-786. [16] FANKHAUSER N, MÄSER P. Identification of GPI anchor attachment signals by a Kohonen self-organizing map[J].Bioinformatics,2005,21(9):1846-1852. [17] PETERSEN T N, BRUNAK S, VON HEIJNE G, et al. Signal P 4.0:discriminating signal peptides from transmembrane regions[J].Nature Methods,2011,8(10):785-786. [18] HORTON P, PARK K J, OBAYASHI T, et al. WoLF PSORT:protein localization predictor[J].Nucleic Acids Research,2007,35(Web Server issue):W585-W587. [19] CROOKS G E, HON G, CHANDONIA J M, et al. WebLogo:a sequence logo generator[J].Genome Research,2004,14(6):1188-1190. [20] 陶泽新,王志玉.冠状病毒包膜蛋白的研究进展[J].预防医学文献信息,2004,10(1):69-73. [21] 叶景荣,徐建国.冠状病毒的生物学特性[J].疾病监测,2005,20(3):160-163. [22] 王明涛,甘一如,黄积涛.神经氨酸苷酶抑制剂研究进展[J].天津理工学院学报,2001,17(2):21-24. [23] JIA X S, XU H D, MIRON R J, et al. EZH1 is associated with TCP-induced bone regeneration through macrophage polarization[J].Stem Cells International,2018:6310560. [24] RUMBAK E, RAWLINGS D E, LINDSEY G G, et al. Characterization of the Butyrivibrio fibrisolvens glgB gene,which encodes a glycogen-branching enzyme with starch-clearing activity[J].Journal of Bacteriology,1991,173(21):6732-6741. [25] 李晓旭,丁苗苗,王猛,等.发菜1,4-α-葡聚糖分支酶(GBE)的基因克隆及其对干旱胁迫的响应[J].分子植物育种,2019,17(19):6305-6313. [26] TUTTLE R L, GILL N S, PUGH W, et al. Regulation of pancreatic beta-cell growth and survival by the serine/threonine protein kinase Akt1/PKBalpha[J].Nature Medicine,2001,7(10):1133-1137. [27] STAVELEY B E, RUEL L, JIN J, et al. Genetic analysis of protein kinase B (AKT) in Drosophila[J].Current Biology,1998,8(10):599-603. [28] 何新,胡晓梅,黄文峰,等.Ras鸟嘌呤核苷酸交换因子1对人结直肠癌细胞增殖、凋亡和细胞周期的影响[J].实用医学杂志,2019,35(1):30-34.
2022, Vol. 41 
