4种LED光源对1龄赤点石斑鱼生长和发育的影响

doi:10.16378/j.cnki.1003-1111.20254

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摘要以1龄赤点石斑鱼为研究对象,将其暴露在白光色温3000 K、红光590 nm、蓝光480 nm、绿光520 nm不同光质LED光源下饲养2个月,比较各条件下鱼的生长及性腺发育的差异。同时,运用qRT-PCR技术,比较各组试验鱼脑和脑垂体中与生长相关的基因(PSS-Ⅰ、NPY和GH),以及与繁殖相关的基因(Kiss1、Kiss2、GPR54、sbGnRH、FSHβ和LHβ)表达量的差异。试验结果显示,白光组试验鱼的体长显著高于蓝光组和绿光组(P<0.05),体质量显著高于绿光组(P<0.05)。试验结束时,白光组试验鱼的GH基因表达量与试验开始时差异不显著(P>0.05),而PSS-Ⅰ基因表达量显著高于试验开始时(P<0.05),表明PSS-Ⅰ基因对GH基因的抑制作用有限。此外,试验结束时,各光质条件下试验鱼脑中NPY基因表达量均显著高于试验开始时(P<0.05),表明4种LED光源均能有效促进试验鱼的食物吸收。试验结束时,各光质条件下试验鱼性腺由染色质核仁期卵母细胞发育至周边核仁期卵母细胞,且脑中GPR54基因表达量以及白光和蓝光组中sbGnRH基因表达量均显著高于试验开始时(P<0.05);但脑垂体中FSHβ和LHβ基因表达量与试验初始时差异不显著(P>0.05),表明其可能对1龄试验鱼繁殖系统的发育无明显作用。试验结果表明,4种LED光源中白光能更好地促进试验鱼的生长,但对其性腺发育无显著促进作用。

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	唐永政

关键词 ： 1龄赤点石斑鱼, LED光源, 生长, 性腺发育, 基因表达量

Abstract：This study used one-year-old red spotted grouper Epinephelus akaara as target species, aimed to investigate the growth and gonadal development of this species exposed to white (3000 K), red (590 nm), blue (480 nm), and green (520 nm) light emitting diodes (LEDs) for two months. The growth and gonadal development were compared. The gene expression of growth-related hormones such as PSS-Ⅰ, NPY and GH, and reproduction-related hormones such as Kiss1, Kiss2, GPR54, sbGnRH, FSHβ, and LHβ in the brain and pituitary was analyzed using qRT-PCR technology. The results showed that at the end of the experiment, the total length of fish in white group was significantly higher than that in blue and green groups (P<0.05), and the body weight was significantly higher than that in green group (P<0.05). There was no significant difference in GH gene level (P>0.05), contrarily, the PSS-Ⅰ gene level was significantly higher than that at the begining of the experiment, suggesting limited effects of PSS-Ⅰ on inhibition of GH synthesis. The significantly increased NPY gene levels in four light groups (P<0.05) indicated that four light quality were effective to stimulate food intake. After two months, the gonad developed from chromatin nucleolar-stage oocytes to perinucleolar-stage oocytes in four light groups. The GPR54 levels in four light groups and sbGnRH levels in white and blue groups were significantly increased when compared to the begining (P<0.05), without significant differences in FSHβ and LHβ gene expression in the four light groups, suggesting that the two genes may not play important roles on the development of reproductive system in the one-year-old red spotted grouper. In summary, the white light was more effective to accelerate the growth of the experimental fish, and may not induce the gonadal development.

Key words： one-year-old red spotted grouper LED light quality growth gonadal development gene expression

收稿日期: 2020-11-17

PACS:

S965.334

基金资助:山东省农业重大应用技术创新项目(SD2019YY003);烟台大学博士科研启动基金资助项目(HX20B29);日本学术振兴会(JSPS)研究项目(26660173).

通讯作者: 唐永政(1967—),男,副教授,硕士生导师;研究方向:鱼类营养和繁殖生理学. E-mail:13906380063@163.com.

作者简介: 徐文刚(1985—),男,讲师,博士;研究方向:石斑鱼繁殖生理学. E-mail:xugang@ytu.edu.cn.

引用本文:

徐文刚, 刘立明, 王九龙, 于文松, 张建柏, 征矢野清, 唐永政. 4种LED光源对1龄赤点石斑鱼生长和发育的影响[J]. 水产科学, 2022, 41(3): 343-351.
XU Wengang, LIU Liming, WANG Jiulong, YU Wensong, ZHANG Jianbai, SOYANO Kiyoshi, TANG Yongzheng. Effects of Four LED Light Sources on Growth and Gonadal Development in One-Year-Old Red Spotted Grouper Epinephelus akaara. Fisheries Science, 2022, 41(3): 343-351.

链接本文:

http://www.shchkx.com/CN/10.16378/j.cnki.1003-1111.20254 或 http://www.shchkx.com/CN/Y2022/V41/I3/343

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