Effect of Nitrite Nitrogen on Infection of Parasite Enterocytozoon hepatopenaei in Ridgetail White Prawn Exopalaemon carinicauda
HU Jihui1, DUAN Jiancheng1, GAO Yang1, CHEN Shuhan1, ZHANG Qingqi2, MU Hua1,3,4, LAI Xiaofang1,3,4, GAO Huan1,3,4
1.Key Laboratory of Marine Biotechnology of Jiangsu Province, College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; 2.Lianyungang Ganyu Jiaxin Aquatic Products Development Co., Ltd., Liangyungang 222100, China; 3.Jiangsu Marine Biological Industry Technology Collaborative Innovation Center, Lianyungang 222005, China; 4.Jiangsu Provincial Platrorm for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China
Abstract:Nitrite nitrogen is one of the important factors to induce the outbreak disease of shrimp. In order to investigate the effects of nitrite stress on the growth status and the carrying capacity of parasite Enterocytozoon hepatopenaei in ridgetail white prawn Exopalaemon carinicauda, five experimental groups with different nitrite nitrogen concentrations (0.008, 3.000, 6.000, 9.000 and 12.000 mg/L) were set up to determine the changes in growth indices and EHP load of ridgetail white prawn in three weeks. The results showed that the survival rate, body length and body weight gain rate of ridgetail white prawn were increased first and then decreased with the increase in nitrite nitrogen concentration. The maximum value was reached in nitrite nitrogen 6.000 mg/L group. The increment of average survival rate, body length and body weight gain rate were 29%, 0.8 cm and 35.33%, respectively. With the increase in nitrite nitrogen concentration, the EHP load of ridgetail white prawn was decreased first and then increased, and reached the lowest value in 6.000 mg/L group, which was 4.4×103 copies/mg. It was found that 6.000 mg/L nitrite nitrogen led to inhibit the transmission of EHP and to promote the growth of ridgetail white prawn. The purpose of this study is to explore the effect of nitrite nitrogen on the transmission rate of EHP, and help to control the concentration of nitrite reasonably in order to inhibit the spread of EHP, so as to improve the survival rate and breeding yield of ridgetail white prawn infected with Enterocytozoon hepatopenaei.
[1]刘珍,张庆利,万晓媛,等.虾肝肠胞虫(Enterocytozoon hepatopenaei)实时荧光定量PCR检测方法的建立及对虾样品的检测[J].渔业科学进展,2016,37(2):119-126. [2]BIJU N, SATHIYARAJ G, RAJ M, et al. High prevalence of Enterocytozoonhepatopenaei in shrimps Penaeus monodon and Litopenaeus vannamei sampled from slow growth ponds in India[J].Diseases of Aquatic Organisms,2016,120(3):225-230. [3]梁静真,贺晓晨,苏栒,等.对虾肝肠胞虫病致病性研究进展[J].广西畜牧兽医,2019,35(1):18-20. [4]姜义仁,邓真华,王伯阳,等.柞蚕微孢子虫孢子分离纯化方法[J].应用昆虫学报,2011,48(2):452-456. [5]程东远.虾肝肠胞虫的流行病学及其致对虾生长缓慢机理的探索[D].上海:上海海洋大学,2017. [6]寇红岩,冼健安,郭慧,等.亚硝酸盐对虾类毒性影响的研究进展[J].海洋科学,2014,38(2):107-115. [7]段健诚,邓高威,张庆起,等.虾类寄生虫病——虾肝肠胞虫研究进展[J].淮海工学院学报(自然科学版),2019,28(3):79-83. [8]栗志民,钱佳慧,刘建勇,等.亚硝酸盐对墨吉明对虾的毒性及其免疫因子的影响[J].安徽农业科学,2015,43(21):160-163. [9]王龙,郝志敏,王晶.两种溶氧条件下亚硝酸盐和氨氮对罗氏沼虾毒性比较的研究[J].饲料与畜牧,2011(8):12-16. [10]潘训彬,张秀霞,鲁耀鹏,等.氨氮和亚硝酸盐对红螯螯虾幼虾和亚成虾的急性毒力[J].生物安全学报,2017,26(4):316-322. [11]陈亭君,刘建勇,申玉春,等.亚硝酸盐氮对日本囊对虾幼虾的急性毒性研究[J].渔业现代化,2019,46(3):35-40. [12]钟君伟,朱永安,付佩胜,等.亚硝酸盐氮对克氏原螯虾成虾的毒性及其抗病因子的影响[J].长江大学学报(自然科学版),2014,11(5):36-40. [13]黄翔鹄,李长玲,郑莲,等.亚硝酸盐氮对凡纳滨对虾毒性和抗病相关因子影响[J].水生生物学报,2006,30(4):466-471. [14]施慧,许文军,谢建军,等.舟山地区大棚凡纳滨对虾生长缓慢病因的调查分析[J].中国水产科学,2017,24(2):387-394. [15]许杰,霍文慧,邓威,等.2015—2017年天津市虾肝肠胞虫流行情况调查[J].中国动物检疫,2018,35(8):13-16. [16]MALLASEN M, VALENTI W C. Effect of nitrite on larval development of giant river prawn Macrobrachium rosenbergii[J].Aquaculture,2006,261(4):1292-1298. [17]葛红星,李健,陈萍,等.亚硝酸盐氮胁迫下凡纳滨对虾对副溶血弧菌的易感性[J].中国水产科学,2014,21(3):629-636. [18]吕晓燕.亚硝酸盐和氨氮对红螯光壳螯虾生理生化的影响[D].上海:华东师范大学,2011. [19]ZHENG J B, MAO Y, SU Y Q, et al. Effects of nitrite stress on mRNA expression of antioxidant enzymes, immune-related genes and apoptosis-related proteins in Marsupenaeus japonicus[J].Fish & Shellfish Immunology,2016,58:239-252. [20]徐胜威,杨程,斯烈钢,等.氨氮胁迫对凡纳滨对虾的生长状况及虾肝肠胞虫(EHP)携带量的影响[J].安徽农业科学,2019,47(7):109-112. [21]徐胜威,杨程,斯烈钢,等.亚硝酸盐对携带虾肝肠胞虫的凡纳滨对虾的影响分析[J].杭州师范大学学报(自然科学版),2019,18(5):510-515. [22]彭自然,臧维玲,高杨,等.氨和亚硝酸盐对凡纳滨对虾幼虾的毒性影响[J].上海水产大学学报,2004,13(3):274-278. [23]路庆鹏,金雪霞,卢萍.养殖水体中亚硝酸盐的来源、危害和控制[J].科学养鱼,2020(8):87. [24]吕晓燕,李嘉尧,方燕,等.亚硝酸盐对红螯光壳螯虾不同组织免疫相关酶活性及超微结构的影响[J].水产学报,2010,34(12):1812-1820. [25]余苗苗,江敏,吴昊,等.亚硝酸盐和氨对日本沼虾肝胰腺代谢的影响[J].生态毒理学报,2019,14(3):99-111. [26]李嘉麒.日本沼虾亚硝酸盐抗性相关基因的筛选与鉴定[D].泰安:山东农业大学,2016. [27]TSENG I T, CHEN J C. The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus under nitrite stress[J].Fish & Shellfish Immunology,2004,17(4):325-333. [28]GUO H, XIAN J N, LI B, et al. Gene expression of apoptosis-related genes, stress protein and antioxidant enzymes in hemocytes of white shrimp Litopenaeus vannamei under nitrite stress[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2013,157(4):366-371. [29]姜宏波,陈裕文,陈启军.虾肝肠胞虫病的研究进展[J].沈阳农业大学学报,2020,51(3):370-376. [30]刘珍.虾肝肠胞虫实时荧光定量PCR检测技术的建立及其与对虾生长相关性的研究[D].上海:上海海洋大学,2015. [31]彭张明,陈妃业,蒲桂川.对虾肝胰腺微孢子虫病与虾肝肠胞虫关系的研究进展[J].水产学杂志,2020,33(5):74-81. [32]WIREDU BOAKYE D, JAROENLAK P, PRACHUMWAT A, et al. Decay of the glycolytic pathway and adaptation to intranuclear parasitism within Enterocytozoonidae microsporidia[J].Environmental Microbiology,2017,19(5):2077-2089. [33]许杰,邓威,李军,等.虾肝肠胞虫(EHP)流行病学与检测技术研究进展[J].中国动物检疫,2018,35(2):64-68. [34]杨铿,冷加华,文国,等.对虾养殖技术之六:对虾养殖中后期亚硝酸盐过高问题的处理与预防[J].中国水产,2008(8):53-55.