Effects of Acute Salinity Stress on Osmoregulation of Juvenile American Shad Alosa sapidissima
GUO Yin1, DAI Xilin2,3,4
1.Shanghai Vocationl College of Agriculture and Forestry, Shanghai 201600, China; 2.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; 3.Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; 4.Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai 201306, China
Abstract：By studying the effects of acute salinity stress on serum osmotic pressure, Na+/K+-ATPase and catalase activities of juvenile American shad Alosa sapidissima were investigated to provide a theoretical basis for controlling the appropriate salinity in American shad culture. The culture experiment was carried out at fresh water and a salinity of 5, 10 and 20 for 96 h, and the changes in serum osmotic pressure, gill Na+/K+-ATPase activity and liver catalase activity were measured in each group by stages. The serum osmotic pressure of juvenile fish showed a significant increase and then decreased and finally gradually stabilized with time in the experimental groups, significantly lower in salinity 5 group than that in salinity 10 and salinity 20 groups (P<0.05). During the experiment, Na+/K+-ATP enzyme activity was decreased significantly and then increased in each group. At 96 h, Na+/K+-ATP enzyme activity was decreased in salinity 20 group, significantly lower than that in salinity 5 and 10 groups (P<0.05). The greater the salinity difference, the longer the upward trend of enzyme activity took. The catalase activity was increased to the peak at 48 h and then decreased in salinity 5 and 10 sroups, the catalase activity of salinity 20 treatment did not increase during the experiment, significantly lower than that in other groups at 48 h (P<0.05). The greater salinity changed, the more easily catalase activity was inhibited. The findings indicated that juvenile American shad had strong osmotic adjustment ability under acute salinity stress at salinity of 5 and 10.
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2022, Vol. 41 
