Effects of Starvation Stress on Immunity and ATPase Activity of Kuruma Prawn Marsupenaeus japonicus
ZHAO Sizhe1, LI Wanying1, LI Yongchuang1, YU Miaomiao1, LAI Xiaofang1,2,3, WANG Panpan1,2,3, YU Fei4, YAN Binlun1,2,3, WU Jun5, HE Xiaofeng6, GAO Huan1,2,3
1. Jiangsu Key Laboratory of Marine Bioresources and Environment, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; 2. Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Lianyungang 222005, China; 3. The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China; 4. Lianyungang Marine and Fishery Development Promotion Center, Lianyungang 222005, China; 5. Lianyungang Qiming Aquatic Products Co., Ltd., Lianyungang 222005, China; 6. Jiangsu Xiaofeng Agricultural Technology Group Co., Ltd., Yancheng 224000, China
Abstract：In this study, kuruma prawn Marsupenaeus japonicus with body weight of (0.8±0.06) g were placed in multi-layer and multi-cell grid culture system at 25 ℃, and one individual was placed in each cell for 0, 3, 6, 9 and 12 days of starvation stress. The changes in various indices in stomach, hepatopancreas and muscle tissues were detected at each time point. The results showed that the starved kuruma prawn had the significantly poor growth performance; catalase (CAT) activities in all tissues were found to be increased first and then decreased under starvation; the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP) were found to be decreased significantly with the prolongation of starvation time in all tissues (P<0.05). The activity of superoxide dismutase (SOD) showed obvious tissue specificity. The activity of SOD in gastric tissue was increased first and then decreased with the extension of starvation time, with the peak value on the third day of starvation. The activity of SOD in hepatopancreas and muscle tissue decreased continuously with the extension of starvation time. With the extension of stress time, the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in various tissues were found to be increased first and then decreased, with the maximal value on the third day of the stress. The findings showed that starvation stress not only significantly reduced the growth performance, but also significantly affected the activities of immune related enzymes and ATPase in different stress periods.
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2023, Vol. 42 
