Abstract：Silver crucian carp Carassius auratus gibelio is one of the excellent varieties of freshwater aquaculture in China. In recent years, the disease problems are becoming more and more serious, and thus have greatly affected the breeding efficiency. Fish infected with diseases can cause changes in the expression levels of some proteins in serum. Therefore, screening some specific proteins as biomarkers is helpful to the identification and control of fish diseases. In the paper, the serum proteins of silver crucian carp were isolated and identified using blue-native polyacrylamide gel electrophoresis and LC-MS/MS. A total of 721 serum proteins were identified. Bioinformatics analysis showed that these proteins were mainly involved in complement and coagulation cascade, metabolic pathway and bacterial infection pathway. A lot of non-specific immunity related proteins including complement C3 proteins and α2-macroglobulin were identified. intensity-based absoluteprotein-quatification (iBAQ) analysis showed that different kinds of complement C3 proteins and α2-macroglobulin had different expression levels. Moreover, some potential biomarkers such as serum transferrin, creatine kinase, α2-enolase and complement factor HF were also determined. The finding provides a new method for the study of serum proteins function, protein-protein interaction and serum biomarker screening for silver crucian carp, which is of great significance for disease prevention of fish.
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2023, Vol. 42 
