Effect of Five Aquaculture Chemicals on Ammonia Nitrogen Removal Efficiency of Bioflocs
LUO Guozhi1,2,3, WU Huifang1, HAO Yaning1, YAO Miaolan1,2,3, TAN Hongxin1,2,3
1. Research and Development Center of Aquaculture Engineering of Shanghai, Shanghai 201306, China; 2. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai201306, China; 3. Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding,Shanghai 201306, China
Abstract：In order to probe for the effect of addition of aquaculture drugs on the ability of bioflocs to remove ammonia nitrogen, conventional doses of florfenicol (0.45 mg/L), doxycycline (1.5 mg/L), sulfamethoxacin (4.8 mg/L), sulfadiazine (4.5 mg/L) and enrofloxacin (15 mg/L) were added into a 10 L plexiglass reactor with a diameter of 20 cm, a height of 50 cm, and a conical bottom containing the biofloc suspension of the tilapia aquaculture system. The dynamics of total ammonia nitrogen (TAN), nitrite nitrogen and nitrate nitrogen in the process of removing 20 mg/L ammonia nitrogen by biological flocs were measured, and the bacterial community structure in each experimental group was compared for 20 days. The reactor containing only the biological floc suspension without the addition of fishery drugs was used as a ontrol group. No significant effect was found in TAN removal for all the five chemicals with their current concentrations under the experimental conditions.. There were significantly higher nitrite nitrogen concentration in reactors with florfenicol and doxycycline than that in the control group (P<0.05), with less nitrite nitrogen concentrations in reactors with sulfamethoxacin, sulfadiazine and enrofloxacin than that in the control group, without significant differences in the richness and diversity of bacterial community in the reactors with sulfamethoxacin, florfenicol and doxycycline, and in the control group (P＞0.05 ). Significantly higher diversity of bacterial community was observed in the reactors with sulfadiazine and enrofloxacin than that in the control reactors (P<0.05),with significantly less richness of bacterial community in these two reactors than that in the control reactors (P<0.05). Among the five chemicals,the most significant difference in the bacterial community was found in the reactors with enrofloxacin (15 mg/L), followed by doxycyclinev (1.5 mg/L) and florfenicol (0.45 mg/L), wihtout significant differences in bacterial community on phylum, class and genus levels between the reactors with the other two chemicals compared to the control group (P＞0.05).
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