Gonadal Development and Changes in Concentration of Serum Sex Hormones in Schizothorax irregularis
ZHAO He1,2,3, ZHAO Nianhua1,2,3, LI Li2,3,4, QIANG Zhuang2,3,4, NIE Zhulan1,2,3, WEI Jie1,2,3
1. College of Animal Science and Technology, Tarim University, Alaer 843300, China; 2. State Key Laboratory BreedingBase for The Protection and Utilization of Biological Resources in Tarim Basin Co-funded by Xinjiang Corps andThe Ministry of Science and Technology, Alaer 843300, China; 3. Key Laboratory of Tarim Animal HusbandryScience and Technology, Xinjiang Production & Construction Corps, Alaer 843300, China; 4. School of Life Science and Technology, Tarim University, Alaer 843300, China
Abstract：To investigate the annual development of testis and ovary of Schizothorax irregularis and the changes in sex hormone content in serum, samples were taken monthly in the Kizil River from August 2020 to August 2021, and a total of 194 samples were obtained. Their testis and ovary were observed by conventional histological method. The otoliths were used as identification material to determine the minimum age at sexual maturity of the fish. The eggs were selected to depict the distribution of egg diameter to determine the spawning type. The results showed that S. irregularis testis and ovary development was divided into six periods; the minimum age at sexual maturity was 2 years for males and 3 years for females, and testis development was earlier than ovary development; S. irregularis spawn from March to May, and the number percentage of egg peaked at 2.9—＜3.1 mm in diameter, and very few mature eggs were present in the ovary at stage Ⅵ after spawning, which was judged to be a fully synchronized spawning type. The maximal serum concentrations of estradiol and testosterone were observed at stage Ⅴ in both females and males. The findings showed that S. irregularis gonads matured and spawned from March to May 2017, and the concentrations of estradiol and testosterone were increased significantly during the reproduction of S. irregularis.
[1]徐钢春,鲍明明,杜富宽,等.鱼类性腺发育及产卵类型研究进展[J].长江大学学报(自然科学版),2017,14(6):43-48. [2]刘晨斌,徐革锋,黄天晴,等.鱼类性腺发育研究进展[J].水产学杂志,2019,32(1):46-54. [3]CASTELLANOS-GALINDO G, BAOS R, ZAPATA L. Mariculture-induced introduction of cobia Rachycentron canadum (Linnaeus, 1766), a large predatory fish, in the Tropical Eastern Pacific[J].BioInvasions Records,2016,5(1):55-58. [4]HAMILTON S, SEVERI W, CAVALLI R O. Biology and aquaculture of cobia:a review[J].Boletim Do Instituto De Pesca Sao Paulo,2013,39(4):461-477. [5]WELTZIEN F A, ANDERSSON E, ANDERSEN Ø, et al. The brain-pituitary-gonad axis in male teleosts, with special emphasis on flatfish (Pleuronectiformes)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2004,137(3):447-477. [6]林浩然.鱼类生理学[M].广州:中山大学出版社,2011:240-248. [7]郭焱.新疆鱼类志[M].乌鲁木齐:新疆科学技术出版社,2012: 106-118. [8]马燕武,郭焱,张人铭,等.新疆塔里木河水系土著鱼类区系组成与分布[J].水产学报,2009,33(6):949-956. [9]魏杰,聂竹兰,李杰,等.塔里木裂腹鱼性腺形态学与组织学的研究[J].大连海洋大学学报,2011,26(3):227-231. [10]方静,何敏,杜仲君,等.齐口裂腹鱼卵巢发育的组织学研究[J].四川农业大学学报,2007,25(1):88-93. [11]谢从新.鱼类学[M].北京:中国农业出版社,2010:334-336. [12]史为良.内陆水域鱼类增殖与养殖学[M].北京:中国农业出版社,1996:44. [13]周贤君.拉萨裂腹鱼个体生物学和种群动态研究[D].武汉:华中农业大学,2014:72. [14]徐伟毅,刘跃天,冷云,等.云南裂腹鱼繁殖生物学研究[J].水利渔业,2006,26(2):32-33. [15]WALLACE R A, SELMAN K. Cellular and dynamic aspects of oocyte growth in teleosts[J].Integrative and Comparative Biology,1981,21(2):325-343. [16]哈承旭,杨翠华,王玮,等.翎电鳗卵巢发育组织学研究[J].水产科学,2021,40(3):403-408. [17]顾海龙,冯亚明,杨智景,等. 长春鳊性腺发育组织学观察[J]. 水产学杂志,2022,35(3): 44-50. [18]唐洪玉,陈大庆,史建全,等.青海湖裸鲤性腺发育的组织学研究[J].水生生物学报,2006,30(2):166-172. [19]何德奎,陈毅峰,蔡斌.纳木错裸鲤性腺发育的组织学研究[J].水生生物学报,2001,25(1):1-13. [20]何德奎,陈毅峰,陈自明,等.色林错裸鲤性腺发育的组织学研究[J].水产学报,2001,25(2):97-102. [21]崔立新,李政海,张靖,等.克孜勒苏河流域植被覆盖空间格局及动态变化趋势分析[J].大连民族大学学报,2019,21(1):39-43. [22]丁城志,陈毅峰,何德奎,等.雅鲁藏布江黑斑原鮡繁殖生物学研究[J].水生生物学报,2010,34(4):762-768. [23]赵贺,洪继彪,赵年桦,等.新疆克孜勒河厚唇裂腹鱼人工繁殖及胚胎、早期仔鱼发育研究[J].大连海洋大学学报,2021,36(3):414-422. [24]AMIRI B M, MAEBAYASHI M, ADACHI S, et al. In vitro steroidogenesis by testicular fragments and ovarian follicles in a hybrid sturgeon, Bester[J]. Fish Physiology and Biochemistry,1999,21(1):1-14. [25]WEBB M A H, FEIST G W, FOSTER E P, et al. Potential classification of sex and stage of gonadal maturity of wild white sturgeon using blood plasma indicators[J].Transactions of the American Fisheries Society,2002,131(1):132-142. [26]赵会宏,刘晓春,刘付永忠,等.斜带石斑鱼雌鱼卵巢发育与血清性类固醇激素的生殖周期变化[J].中山大学学报(自然科学版),2003,42(6):56-59. [27]徐文刚,唐永政,张建柏,等.黑边石斑鱼卵巢周年发育及血清中性激素含量的变化[J].大连海洋大学学报,2020,35(5):657-662. [28]MIURA T, MIURA C I. Molecular control mechanisms of fish spermatogenesis[J].Fish Physiology and Biochemistry,2003,28(1/2/3/4):181-186. [29]MIURA T, MIURA C, OHTA T, et al. Estradiol-17beta stimulates the renewal of spermatogonial stem cells in males[J].Biochemical and Biophysical Research Communications,1999,264(1):230-234. [30]王四泽,张颖,耿龙武,等.大鳞鲃生长、血清性激素水平和性腺发育的研究[J].大连海洋大学学报,2016,31(4):351-356.
2023, Vol. 42 
