Abstract：Klebsiella pneumonia is the second largest conditioned pathogen after Escherichia coli and infects a wide range of hosts, including humans and a variety of animals. In recent years, diseases caused by K. pneumoniae infection of aquatic animals have occurred frequently, which has seriously hindered the healthy development of aquaculture. In this study, the method of bacterial isolation, culture and morphological observation were used to isolate a suspected pathogenic bacterium (designated as KPLYC2) from the liver of cultured diseased large yellow croaker Larimichthys crocea for identification. Large yellow croakers were infected by injection with KPLYC2, which caused the same symptoms and death of natural infection. The medial lethal dose of KPLYC2 was 6.8×106 cfu/mL, and caused obvious pathological damage to the liver, kidney and spleen in large yellow croaker. KPLYC2 also infected mice and caused systemic pathological damage. Through the physiological and biochemical characteristics detection, 16S rRNA sequence analysis and construction of phylogenetic tree, it was found that the genetic identity was more than 99% between the isolated strain and K. pneumoniae, so KPLYC2 was identified as K. pneumoniae. The drug sensitivity test showed that KPLYC2 was not sensitive to ampicillin, penicillin G and tetracycline. A strain of K. pneumoniae was isolated from large yellow croaker for the first time and its drug resistance and pathogenicity was explored. The finding will provide reference and foundation for the prevention and control of this disease.
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2023, Vol. 42 
