Effects of Curcumin on Growth Performance and Non-Specific Immunity of Largemouth Bass Micropterus salmoides
ZHANG Meiyan1,2, YANG Xing1,2, JIANG Zaisheng3, ZHAO Feng1,2, LI Songyan4, LIU Tingting4, ZHANG Zhixin4, SHANG Baodi1,2
1. Institute of Fisheries, Guizhou Academy of Agricultural Sciences, Guiyang 550025; China; 2. Special Aquaculture of Engineering Technology Center of Guizhou, Guiyang 550025, China; 3. Extending Stations of Aquaculture Technology of Agriculture and Rural Bureau in Xuanzhou in Anhui, Xuancheng 242000, China; 4. Guizhou Normal University, Guiyang 550018, China
Abstract：The 60-day feeding trial was conducted to study the effects of different dietary curcumin levels on growth performance, and non-specific immunity for largemouth bass Micropterus salmoides with body weight of (4.17±0.39) g. Purified basal diets were formulated, containing curcumin of 0(control), 15, 30, 60, 120, and 240 mg/kg diet. Each diet was fed to triplicate groups, 30 fish each group. The results showed that: (1)compared with the control group, the weight gain rate (WGR) and specific growth rate (SGR) were significantly higherin 60 mg/kg groupthan that in the others (P<0.05), and the FCR(feed conversion ratio) was lowest in 30 mg/kg and 60 mg/kg groups, but there was no significant differences in viscerosomatic index (VSI), hepatosomatic index (HSI) among the experimental groups (P>0.05). (2)White blood cell (WBC) number were significantly higherin 30 mg/kg and 60 mg/kg groupsthan that in control group (P<0.05), there was no significant differences in red blood cell (RBC) and HGB (hemoglobin) among all groups (P>0.05). (3)With the increase in dietary curcumin supplementation, the alanine amino transferase (ALT) and aspartate amino transferase (AST) activities were decreased first and then increased, alanine amino transferase (ALT) reached the lowest level when dietary curcumin supplementation was 60 mg/kg than others (P<0.05), and aspartate amino transferase (AST) reached the lowest level when dietary curcumin supplementation were 60 mg/kg and 120 mg/kg(P<0.05). There were no significant differences in total protein (TP) and globular proteins (GLB) among groups (P>0.05). (4)Compared with the control group, the activities of ACH50 in 30, 60 and 120 mg/kg groups were significantly higher (P<0.05), and in groups with curcumin TNF-α were significantly higher than that in the control group (P<0.05). The IL-1β activity was the highest in 60 mg/kg group. (5)In 60 mg/kg group, the expression of PrxⅠ, PrxⅡ, PrxⅣ, PrxⅤ, and PrxⅥ genes was significantly increased (P<0.05). In conclusion, the dietary with 60 mg/kg curcumin can improve the growth performance, non-specific immune response and antioxidant capacity of M. salmoides, which is the optimal dietary supplemental level.
[1]MING J H, XIE J, XU P, et al. Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala Yih) under high temperature stress[J].Fish & Shellfish Immunology,2012,32(5):651-661. [2]CHAINANI-WU N. Safety and anti-inflammatory activity of curcumin:a component of tumeric (Curcumalonga)[J].Journal of Alternative and Complementary Medicine,2003,9(1):161-168. [3]PAN M H, HUANG T M, LIN J K. Biotransformation of curcumin through reduction and glucuronidation in mice[J].Drug Metabolism and Disposition:the Biological Fate of Chemicals,1999,27(4):486-494. [4]GUPTA S C, PRASAD S, KIM J H, et al. Multitargeting by curcumin as revealed by molecular interaction studies[J].Natural Product Reports,2011,28(12):1937-1955. [5]刘素标.姜黄素的作用研究进展[J].中医临床研究,2013,5(4):117,120. [6]CUI H, LIU B, GE X, et al. Effects of dietary curcumin on growth performance, biochemical parameters, HSP70 gene expression and resistance to Streptococcus iniae of juvenile Gift Tilapia, Oreochromis niloticus[J].The Israeli Journal of Aquaculture-Bamidgeh,2013(66):986-996. [7]欧红霞,王广军,李志斐,等.不同饲料对大口黑鲈肠道组织结构的影响[J].水产科学,2020,39(6):902-907. [8]韩晓磊,王浩,高俊杰,等.工厂化循环水养殖条件下大口黑鲈生长特点分析[J].水产科学,2020,39(4):567-572. [9]WANG X S, PHELAN S A, FORSMAN-SEMB K, et al. Mice with targeted mutation of peroxiredoxin 6 develop normally but are susceptible to oxidative stress[J].The Journal of Biological Chemistry,2003,278(27):25179-25190. [10]MATSUYAMA H, TANAKA K, NAKAO M, et al. Characterization of the alternative complement pathway of carp[J].Developmental & Comparative Immunology,1988,12(2):403-408. [11]YANO T, HATAYAMA Y, MATSUYAMA H, et al. Titration of the alternative complement pathway activity of representative cultured fishes[J].Nippon Suisan Gakkaishi,1988,54(6):1049-1054. [12]崔素丽.高温下大黄素对团头鲂生长、血液指标及抗氨氮应激的影响[D].南京:南京农业大学,2012. [13]PLATEL K, SRINIVASAN K. Influence of dietary spices or their active principles on digestive enzymes of small intestinal mucosa in rats[J].International Journal of Food Sciences and Nutrition,1996,47(1):55-59. [14]罗霜,于小玲,李红静.姜黄素对小鼠胃肠蠕动的影响及其作用机制[J].齐鲁医学杂志,2012,27(5):430-431. [15]LIU B, GE X P, HE Y H, et al. Effects of anthraquinones extracted from Rheum officinale Bail on the growth, non-specific immune response of Macrobrachium rosenbergii[J].Aquaculture,2010,310(1/2):13-19. [16]XIE J, LIU B, ZHOU Q L, et al. Effects of anthraquinone extract from rhubarb Rheum officinale Bail on the crowding stress response and growth of common carp Cyprinus carpio var. Jian[J].Aquaculture,2008,281(1/2/3/4):5-11. [17]MAHMOUD H K, AL-SAGHEER A A, REDA F M, et al. Dietary curcumin supplement influence on growth, immunity, antioxidant status, and resistance to Aeromonas hydrophila in Oreochromis niloticus[J].Aquaculture,2017,475:16-23. [18]BANERJEE A, KUNWAR A, MISHRA B, et al. Concentration dependent antioxidant/pro-oxidant activity of curcumin[J].Chemico-Biological Interactions,2008,174(2):134-139. [19]胡忠泽,杨久峰,谭志静,等.姜黄素对草鱼生长和肠道酶活力的影响[J].粮食与饲料工业,2003(11):29-30. [20]沃兴德,洪行球,高承贤.姜黄素最大耐受量试验[J].浙江中医学院学报,2000,24(2):55. [21]NYA E J, AUSTIN B. Use of dietary ginger, Zingiber officinale Roscoe, as an immunostimulant to control Aeromonas hydrophila infections in rainbow trout, Oncorhynchus mykiss (Walbaum)[J].Journal of Fish Diseases,2009,32(11):971-977. [22]NYA E J, AUSTIN B. Use of garlic, Allium sativum, to control Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum)[J].Journal of Fish Diseases,2009,32(11):963-970. [23]YILDIRIM-AKSOY M, LIM C, SHELBY R, et al. Increasing fish oil levels in commercial diets influences hematological and immunological responses of channel catfish, Ictalurus punctatus[J].Journal of the World Aquaculture Society,2009,40(1):76-86. [24]JHA A K, PAL A K, SAHU N P, et al. Haemato-immunological responses to dietary yeast RNA, ω-3 fatty acid and β-carotene in Catla catla juveniles[J].Fish & Shellfish Immunology,2007,23(5):917-927. [25]TALPUR A D, IKHWANUDDIN M. Dietary effects of garlic (Allium sativum) on haemato-immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian sea bass, Lates calcarifer (Bloch)[J].Aquaculture,2012,364/365:6-12. [26]KIM Y, YOU Y, YOON H G, et al. Hepatoprotective effects of fermented Curcuma longa L. on carbon tetrachloride-induced oxidative stress in rats[J].Food Chemistry,2014,151:148-153. [27]李赫,宋文华,张健,等.姜黄素对草鱼生长性能和免疫指标的影响[J].科学养鱼,2018(3):24-26. [28]夏斯蕾.姜黄素对团头鲂生长、非特异性免疫及抗氧化蛋白基因(Prx)表达的影响[D].南京:南京农业大学,2015:31. [29]张媛媛,宋理平,胡斌,等.饲料中添加姜黄素对尼罗罗非鱼幼鱼生长和四氯化碳诱导肝损伤的影响[J].中国水产科学,2018,25(6):1271-1280. [30]刘国安,许欣欣,李贵琛,等.姜黄素在生化和细胞体系的抗氧化与促氧化作用[J].西北师范大学学报(自然科学版),2020,56(3):82-89. [31]张媛媛,刘波,周传朋,等.团头鲂对营养需求的研究进展[J].安徽农业科学,2010,38(32):18239-18241. [32]SRIVASTAVA R M, SINGH S, DUBEY S K, et al. Immunomodulatory and therapeutic activity of curcumin[J].International Immunopharmacology,2011,11(3):331-341. [33]RAMIREZ-TORTOSA M C, RAMIREZ-TORTOSA C L, MESA M D, et al. Curcumin ameliorates rabbits′s steatohepatitis via respiratory chain, oxidative stress, and TNF-Α[J].Free Radical Biology and Medicine,2009,47(7):924-931. [34]WHYTE S K. The innate immune response of finfish: a review of current knowledge[J].Fish & Shellfish Immunology,2007,23(6):1127-1151. [35]高世勇,李丹.肿瘤坏死因子与癌症相关研究进展[J].中国药理学通报,2020,36(9):1209-1213. [36]丰培金.鱼类的白细胞介素研究进展[J].安徽农业科学,2006,34(17):4317-4318. [37]ELLIS A E. Innate host defense mechanisms of fish against viruses and bacteria[J].Developmental & Comparative Immunology,2001,25(8/9):827-839. [38]王荣春,韩利文,何秋霞,等.利用斑马鱼模型研究姜黄素抗炎的分子机制[J].中国药理学与毒理学杂志,2016,30(10):1031. [39]WANG J, KANG Y X, PAN W, et al. Enhancement of anti-inflammatory activity of curcumin using phosphatidylserine-containing nanoparticles in cultured macrophages[J].International Journal of Molecular Sciences,2016,17(5):969. [40]章波,向渝梅,白云.抗氧化蛋白Peroxiredoxin家族研究进展[J].生理科学进展,2004,35(4):352-355. [41]王玉堂.姜黄素及其在水产养殖业的应用[J].中国水产,2016(8):85-87.
2023, Vol. 42 
