Acute Toxicity of Four Conventional Disinfectants in Aquaculture on Tadpoles of Frog Quasipaa spinosa
XIE Yongguang, WEI Zhaoyu, WEI Xiuying, LUO Huahui, CHEN Dunxue
Key Laboratory of Animal Genetics and Breeding and Reproduction of Plateau and Mountain Animals of Guizhou University, Ministry of Education, Research Center of Fishery Resources and Environment of Guizhou University, College of Animal Science, Guizhou University, Guiyang 550025, China
Abstract：As an important aquaculture species, the aquaculture scale of amphibians is expanding, and the use of disinfectant has been inevitable. In order to evaluate acute toxicity of conventional disinfectants in aquaculture on amphibian larvae, the tadpoles of frog Quasipaa spinosa at Gosner stage 38—39 were exposed to the water contaminated in sodium chloride, formaldehyde, potassium permanganate and povidone-iodine by a static test method. The results showed that the order of median-lethal concentration(LC50)of the four conventional disinfectants were expressed as the following: sodium chloride>formaldehyde>potassium permanganate>povidone-iodine. The 96 h median lethal concentrations (LC50) was shown to be 7145.53 mg/L, 16.32 mg/L, 3.61 mg/L and 2.93 mg/L with safe concentra-tion (SC) of 1949.01mg/L, 4.80mg/L, 0.88mg/L and 1.85 mg/L respectively. Potassium permanganate and povidone-iodine were within the medium toxic substances while the sodium chloride and formaldehyde within the low toxic substances for tadpoles of the frog based on the toxic classification standards published by State Environmental Protection Administration of China. Taking full account of the dose-sensitivity and established conventions, povidone iodine is recommended as a disinfectant. In addition, the tadpoles are more sensitive to fish, so it needs to reduce concentration of disinfectants in amphibian larvae.
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2023, Vol. 42 
