DNA Barcode for Species Identification and Phylogenetic Relationship of Bagridae Fish
LIU Hongyan, XIONG Fei, ZHAI Dongdong, WANG Ying, XIA Ming, CHEN Yuanyuan
1. Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, School of Life Sciences, Jianghan University, Wuhan 430056, China; 2. Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Jianghan University, Wuhan 430056, China
Abstract：The family Bagridae is a diverse fish group, some species with highly similar traits are difficult to be identified and the phylogenetic relationship are ambiguity. Therefore, an accurate and convenient method for identifying and classifying is important and necessary. Herein, we provided a molecular method based on mitochondrial cytochrome c subunit Ⅰ (COⅠ) for the identification and analysis of phylogenetic relationship of Bagridae fish. Two hundred and fourteen mitochondrial COⅠ gene sequences belonging to thirty-six species of five genera were studied. The average genetic distances of intraspecific and interspecific species were 0.016 and 0.158, respectively. The average interspecific genetic distance was 9.875 times of the average intraspecific genetic distance. It existed barcode gaps between interspecific distance and intraspecific distance. ABGD analysis showed that 36 species were divided into 25 operational taxonomic units (OTU), The sorting of OTU were roughly consistent with the genetic distance method. The interspecific genetic distance of several species forming one OTU were small, and the intraspecific genetic distance of one species separating into several OTUs were large. The members of Pelteobagrus, Pseudobagrus and Leiocassis mixed together and clustered into one large branch. There was one species each clustering into opposite genus in the genus of Mystus and Hemibagrus, which indicated that it existed incomplete lineage sorting in the two genera. The results indicated that most of Bagridae species could be identified by DNA barcode, but there were limitations in barcode identification of some closely related species. In addition, DNA barcoding had clarified the evolutionary relationships and would provide a scientific reference for phylogenetic classification of Bagridae fish.
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2023, Vol. 42 
