Dynamic Changes in Physical-Chemical Factors and Microbial Diversity in Ecological Culture Ponds of Largemouth Bass Micropterus salmoides
ZHANG Guangchen1, WANG Yang1,2, SUN Xueliang1,2, LUO Yunlong1, WANG Jingru1, LIU Fanxiang1, JI Yanbin1,2
1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China; 2. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Tianjin 300392, China
Abstract：To explore the changes of microorganisms and water quality physicochemical indices in ecoculture ponds of largemouth bass Micropterus salmoides in July—September and their relationship, the microbial community structure was determine by high throughput sequencing technology and the physico-chemical indices were monitored in surface water in the cultured areas and microecosystem of a largemouth bass ecological culture pond in Tianjin from July to September. After the aquaculture tail water was treated by aquatic vegetables, the surface water entered the micro-ecological filtration area with brush as the matrix, and then the bottom water entered the aquatic vegetable treatment area and finally entered the 300 m2 culture pond with4000 individuals of largemouth bass for recycling. The results showed that there was always higher α diversity of microbial diversity in the sediment than that in water and brush, with the dominant microorganisms in Proteobacteria in all samples. Cyanobacteria in the water was found to be gradually increased to the dominant species in September, with change in the dominant bacteria in the brush samples with the time of cultivation and with the maximal proportion of Firmicutes in September. The maximal content of ammonia nitrogen was observed in the water in the aquaculture area and the microecological area in August, with the maximal contents of total nitrogen, orthophosphates, permanganate index, and suspended solids (SS), and pH and total alkalinity in September in the two regions. The correlation analysis showed that there was a significant negative correlation between the nitrate nitrogen content in the water and the Rhizobiales Incertae Sedis abundance in the brush. There was significant negative correlation between the content of nitrite nitrogen and the abundance of Rhodobacteraceae in sediment and Norank_o_ _norank_c_ _Nitrospira in brush (P<0.05). The findings provide a reference for the regulation of water quality and microorganisms in ecological ponds and the subsequent related studies, and the screening of functional bacteria for remediation of water quality in largemouth bass from July to September
张光晨, 王洋, 孙学亮, 罗云龙, 王竞儒, 刘凡湘, 季延滨. 大口黑鲈生态塘微生态及水理化指标变化分析[J]. 水产科学, 2023, 42(4): 622-631.
ZHANG Guangchen, WANG Yang, SUN Xueliang, LUO Yunlong, WANG Jingru, LIU Fanxiang, JI Yanbin. Dynamic Changes in Physical-Chemical Factors and Microbial Diversity in Ecological Culture Ponds of Largemouth Bass Micropterus salmoides. 水产科学, 2023, 42(4): 622-631.
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