Substance Exchange between Water-Vascular System and Coelom in Sea Cucumber Apostichopus japonicus
FAN Xuyuan1,2, WANG Zhenhui2, REN Yuan2,3, GUO Liyuan1,2, SUN Qirui1,2, WANG Yinan2, LI Qiang2
1. Dalian Ocean University, Dalian 116023, China; 2. College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China; 3. School of Bioengineering, Dalian University of Technology, Dalian 116024, China
Abstract：Sea cucumber Apostichopus japonicus with a body mass of (65±10) g was injected in coelom with beet root pigments—beetroot red, purified and adjusted GST-tagged protein, fluorescent microsphere dilution, suspension of Vibrio splendidus and suspension of Escherichia coli. The distribution of the injected matters in the coelom and the water-vascular system (represented by polian vesicle) was detected to explore the correlation, connectivity and material exchange between the coelom and the water-vascular system of the sea cucumber. The results showed that the coelomic fluid from coelom and polian vesicle (hereinafter referred to as “vesicle fluid”) were stained into red at 6 hours after injection by beet red dye. A large number of GST-tag proteins were detected in the coelomic fluid, and there were also some proteins in the vesicle fluid 6 h and 12 h after protein injection. Many fluorescent microspheres were observed in the coelomic fluid, but not in the vesicle fluid 6 h and 12 h after injection. In addition, a large number of E. coli and V. splendidus bacteria were found in the coelomic fluid 6, 12, 24 and 72 h after injection, without bacteria isolated in the vesicle fluid. In conclusion, the water-vascular system and the coelom are not found to be infinitely unblocked in sea cucumber. Soluble substances such as small molecules and biological macromolecules were exchanged between the coelom and the water-vascular system, but the exogenous particles and bacteria did not enter the water-vascular system via the coelom. The findings laid a theoretical foundation for the subsequent study of the physiological structure and biological role of the water-vascular system in sea cucumber.
范栩源, 王振辉, 任媛, 国丽媛, 孙启睿, 王轶南, 李强. 仿刺参水管系统与体腔间物质交换问题探究[J]. 水产科学, 2023, 42(4): 705-711.
FAN Xuyuan, WANG Zhenhui, REN Yuan, GUO Liyuan, SUN Qirui, WANG Yinan, LI Qiang. Substance Exchange between Water-Vascular System and Coelom in Sea Cucumber Apostichopus japonicus. 水产科学, 2023, 42(4): 705-711.
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2023, Vol. 42 
