Genetic Diversity of Stay Carp Cyprinus carpio Adapted to Paddy Field Conditions
ZOU Li1, WANG Jinlong1,2, LI Chuanwu1, WANG Dongwu1, ZENG Chunfang1, LIU Mingqiu1, LIU Li1, XIE Min1, ZENG Ming1
1. Fisheries Research Institute of Hunan Province, Changsha 410153, China; 2. Changde Research Center for Agricultural Biomacromolecule, Zoology Key Laboratory of Hunan Higher Education, Hunan Provincial Key Laboratory for Health Aquaculture and Product Processing in Dongting Lake Area, Hunan University of Arts and Science, Changde 415000, China
Abstract：In order to investigate the genetic information on the stay carp Cyprinus carpio adapted to paddy field conditions in Hunan and Guizhou, fourteen microsatellites were used to evaluate the genetic diversity and genetic differentiation of three “stay carp” populations from Yongshun Xiangxi, Outuan Huaihua, Jinping Qiandongnan, one red carp population from Fisheries Research Institute of Hunan Province, one wild stay carp population from Xiangjiang. The results showed that 14 pairs of microsatellite primers were amplified with clear bands 16.571—19.214 average numbers of alleles, and 9.420—11.143 effective alleles per pair. The average observed heterozygosity was from 0.705 to 0.778, while mean expected heterozygosity ranged from 0.884 to 0.893, the value of average polymorphism information content was from 0.883 to 0.891. The genetic differentiation index among the 5 populations, the gene flow values at all locihe genetic variation among populations, variation within populations. These findings that the 5 carp populations had high level of genetic diversity, of gene flow in the 5 populations, and genetic differentiation. The “stay carp” population still kept high genetic diversity, and genetic differentiation with wild population from Xiangjiang.
[1] 郭梁,任伟征,胡亮亮,等.传统稻鱼系统中“田鲤鱼”的形态特征[J].应用生态学报,2017,28(2):665-672. [2] 甘宝江,张盛,韦玲静,等.广西稻田养殖金边鲤群体遗传多样性分析[J].水产科学,2019,38(5):636-646. [3] 湘西自治州地方志编纂委员会. 湘西土家族苗族自治州志丛书:畜牧水产志[M].长沙:湖南出版社,1996:84-85. [4] 王沈同,沈玉帮,孟新展,等.草鱼野生与选育群体遗传变异微卫星分析[J].水产学报,2018,42(8):1273-1284. [5] 韩承慧,马海涛,姜海滨,等.许氏平鲉(Sebastes schlegeli)微卫星标记开发及野生、养殖群体遗传多样性分析[J].海洋与湖沼,2016,47(1):213-220. [6] 宋娜,都基隆,王志勇,等.香鱼野生群体和养殖群体遗传多样性比较[J].水产学报,2014,38(1):41-46. [7] 董在杰,刘念,傅建军,等.6个野生与选育鲤群体的微卫星遗传分析[J].南方水产科学,2018,14(4):46-55. [8] 马冬梅,苏换换,朱华平,等.华南鲤选育群体不同世代遗传多样性与遗传结构的微卫星分析[J].水生生物学报,2018,42(5):887-895. [9] 史君洁,石连玉,李池陶,等.微卫星标记对4种鲤鱼种质鉴定技术的研究[J].基因组学与应用生物学,2018,37(2):748-755. [10] 顾颖,鲁翠云,张芹,等.微卫星QTL标记分析豫选黄河鲤群体遗传结构及生长性状相关性[J].淡水渔业,2016,46(4):9-18. [11] 鲁翠云,李超,曹顶臣,等.标记指导大规模混合家系选育技术应用实例[J].水产学杂志,2019,32(5):8-12. [12] 萨姆布鲁克.分子克隆实验指南[M].3版. 北京:科学出版社,2016. [13] CROOIJMANS R P M A, DER POEL J J V, GROENEN M M M, et al. Microsatellite markers in common carp (Cyprinus carpio L.)[J].Animal Genetics,1997,28(2):129-134. [14] 侯宁,张研,鲁翠云,等.微卫星DNA标记分析德国镜鲤的遗传潜力[J].遗传,2007,29(12):1509-1518. [15] 鲁翠云,毛瑞鑫,李鸥,等.鲤鱼三、四核苷酸重复微卫星座位的筛选及特征分析[J].农业生物技术学报,2009,17(6):979-987. [16] BOTSTEIN D, WHITE R L, SKOLNICK M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3):314-331. [17] 罗文永,胡骏,李晓方.微卫星序列及其应用[J].遗传,2003,25(5):615-619. [18] 全迎春,孙效文,梁利群.应用微卫星多态分析四个鲤鱼群体的遗传多样性[J].动物学研究,2005,26(6):595-602. [19] 丁严冬,王佩佩,贾秀琪,等.河川沙塘鳢五个家系遗传多样性的微卫星分析[J].水产科技情报,2016,43(3):113-118. [20] 胡雪松,李池陶,马波,等.3个德国镜鲤养殖群体遗传变异的微卫星分析[J].水产学报,2007,31(5):575-580. [21] 李琳.珠江三种鲌亚科鱼类的微卫星特征及广东鲂遗传多样性分析[D].上海:上海海洋大学,2014. [22] 胡玉婷,江河,段国庆,等.安徽两水系黄颡鱼的微卫星遗传多样性分析[J].南方水产科学,2020,16(5):33-41. [23] 桑滨,鲁翠云,李超,等.微卫星标记分析3个耐寒鲤品种的遗传多样性[J].生物学杂志,2017,34(3):24-32. [24] 刘臻,鲁双庆,匡刚桥,等.湘江野鲤养殖群体和自然群体遗传多样性的微卫星分析[J].生态学杂志,2007,26(7):1074-1079. [25] 曾繁振,俞小牧,童金苟.三个鲤品种微卫星丰度与遗传多样性分析[J].水生生物学报,2013,37(5):967-973. [26] 马秀英,王伟,庞有志,等.两个黄河鲤鱼群体遗传多样性的微卫星标记分析[J].黑龙江畜牧兽医,2016(23):22-26. [27] 杨宇晖,梁旭方,林群,等.广东与江西翘嘴鳜养殖与天然种群的遗传多态性分析[J].水产学报,2010,34(4):515-520. [28] 武菲,胡文革,王翠华,等.河鲈养殖与野生群体遗传多样性比较分析[J].水生生物学报,2016,40(1):181-188. [29] 池炳杰,常玉梅,闫学春,等.瓦氏雅罗鱼达里湖群体和乌苏里江群体的遗传多样性和遗传结构分析[J].中国水产科学,2010,17(2):228-235. [30] 夏建海,范武江,王晓清,等.3个不同地理日本沼虾天然群体遗传多样性的SSR分析[J].湖南师范大学自然科学学报,2014,37(4):23-28. [31] 孙成飞,叶星,董浚键,等.罗氏沼虾6个养殖群体遗传多样性的微卫星分析[J].南方水产科学,2015,11(2):20-26. [32] 傅建军,李家乐,沈玉帮,等.草鱼野生群体遗传变异的微卫星分析[J].遗传,2013,35(2):192-201.
2023, Vol. 42 
