Effects of Green Alga Chlorella pyrenoidosa on Growth Performance, Immune Function, Digestive Enzyme Activities and Muscle Quality of Grass Carp Ctenopharyngodon idellus
QUE Jianglong1,2, RAO Yi1, XU Xiandong1, DING Liyun1, ZHANG Haixin1, ZHANG Aifang1, LI Yanfang1
1. Fisheries Science Research Institute of Jiangxi Province, Nanchang 330039, China; 2. Jiangxi Protection and Rescue Center of Hydrobiology, Nanchang 330096, China
Abstract：The objective of this study was to investigate the effects of different levels of Chlorella pyrenoidosa supplementation on growth performance, non-specific immune function, digestive enzyme activities and muscle quality of grass carp Ctenopharyngodon idellus. Grass carps with an average body weight of (105.92±2.79) g were randomly divided into 5 groups (30 fish per group) and fed diets supplemented with 0 (control group), 0.25%, 0.50%, 0.75% and 1.00% C. pyrenoidosa, respectively, for 28 days. The results showed that the average final body weight, weight gain rate (WGR) and specific growth rate (SGR) in 0.50%, 0.75% and 1.00% supplemental groups were significantly higher than those in control group (P<0.05), and the feed conversion ratio (FCR) in 0.50%, 0.75% and 1.00% supplemental groups were significantly lower than those in control group (P<0.05). When the addition amount reaches 0.75%, the activities of lysozyme and alkaline phosphatase in serum of grass carp were significantly increased, and the non-specific immune function of grass carp was enhanced (P<0.05). The activity of superoxide dismutase and antioxidant capacity in serum of all groups were significantly increased except 0.25% group. The activities of lipase and trypsin were increased in all groups, while the activities of amylase (except 0.25% group) were significantly decreased (P<0.05). There was no significant difference in amino acid composition between muscle and control group (P>0.05). The contents of linolenic acid and eicosapentaenoic acid (EPA) in 1.00% group were significantly higher than those in control group (P<0.05), and the contents of arachidonic acid and docosahexaenoic acid (DHA) in 0.25%, 0.75% and 1.00% groups were significantly higher than those in control group (P<0.05). In conclusion, adding a certain amount of C. pyrenoidosa to the feed can promote the growth performance, non-specific immune function, antioxidant capacity and muscle quality of grass carp. With the weight gain rate and feed conversion ratio as evaluation index, the optimal supplemental level of C. pyrenoidosa in the feed of grass carp is 0.75%.
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2023, Vol. 42 
