Abstract：Effects of antibiotic medicine bath on gut microbiota of juvenile sea cucumber Apostichopus japonicus wereexplored in this study. When changing the breeding pond, the common group and the medicine bath group were set. The juvenile sea cucumber in the common group were cultured normally without medicine bath. The juvenile sea cucumber in the medicine bath group were bathed with florfenicol at the concentration of 0.1 g/L for 15 min. Two groups of intestinal samples were taken at 24, 48 and 72 h after changing the breeding pond for 16S rDNA high-throughput sequencing analysis. The results showed that the gut microbiota structure of juvenile sea cucumber in the common group were basically the same as that of juvenile sea cucumber before changing the breeding pond. The gut microbiota diversity of juvenile sea cucumber in the medicine bath group changed greatly, with the maximal bacterial diversity at 24 h, then decreased gradually. The relative abundance of the dominant bacterial phylum Proteobacteria in juvenile sea cucumber was more than 98%. At 24, 48 and 72 h after the medicine bath, the relative abundance of the Proteobacteria in juvenile sea cucumber were 90.51%, 92.99% and 96.63%, respectively. The relative abundance of Actinobacteria, Planctomycetes, Bacteroidetes, Acidobacteria, Chloroflexi, Verrucomicrobia and Firmicutes was increased, and the new detection of Dadabacteria, Dependentiae and Gemmatimonadetes. The relative abundance of dominant bacteria Noviherbaspirillum in the intestines of juvenile sea cucumber in the common group were more than 95%. The relative abundance of Noviherbaspirillum in the intestines of juvenile sea cucumber was decreased to 2.46%, and Phyllobacterium, Candidatus Competitor and Sphingomonas became new dominant bacteria at 24 h after the medicine bath. The relative abundance of Noviherbaspirillum was increased to 36.7% at 48 h and 69.9% at 72 h after the medicine bath, others gradually decreased, and the gut microbiota structure gradually recovered to that before the medicine bath. Antibiotic bath was suggested to break the balance of gut microbiota structure of juvenile sea cucumber, which may affect the digestion and absorption of juvenile sea cucumber and increase the risk of disease. Antibiotic bath is not recommended when changing the breeding pond. The finding provides theoretical basis and technical support for healthy culture of sea cucumber.
李石磊, 杨慧花, 张乾, 王旭达, 董颖, 叶博, 赵振军, 王笑月, 刘丹妮, 曹琛, 周遵春. 药浴对仿刺参幼参肠道菌群的影响[J]. 水产科学, 2023, 42(5): 871-877.
LI Shilei, YANG Huihua, ZHANG Qian, WANG Xuda, DONG Ying, YE Bo, ZHAO Zhenjun, WANG Xiaoyue, LIU Danni, CAO Chen, ZHOU Zunchun. Effects of Medicated Bath on Gut Microbiota of Juvenile Sea Cucumber Apostichopus japonicus. 水产科学, 2023, 42(5): 871-877.
[1] 隋锡林.海参增养殖[M].北京:农业出版社,1990. [2] 常亚青,丁君,宋坚,等.海参、海胆生物学研究与养殖[M].北京:海洋出版社,2004. [3] 高菲,孙慧玲,许强,等.刺参消化道内含物细菌群落组成的PCR-DGGE分析[J].中国水产科学,2010,17(4):671-680. [4] 丁斯予,王荦,徐瀚晨,等.刺参肠道及养殖环境菌群结构与功能[J].生态学杂志,2019,38(1):210-220. [5] WANG L, WEI C, CHANG Y Q, et al. Response of bacterial community in sea cucumber Apostichopus japonicus intestine, surrounding water and sediment subjected to high-temperature stress[J].Aquaculture,2021,535:736353. [6] 李建光,徐永平,李晓宇,等.不同养殖季节仿刺参肠道与养殖环境中菌群结构的特点[J].水产科学,2014,33(9):562-568. [7] 全紫娇,高萍萍,张杨,等.不同温度下刺参肠道菌群结构与功能[J].生态学杂志,2019,38(9):2756-2764. [8] 柴英辉,高菲,王金锋,等.仿刺参(Apostichopus japonicus)肠道菌群的地域性差异与共性研究[J].海洋与湖沼,2019,50(5):1127-1137. [9] 陆振.福建与山东、辽宁养殖仿刺参肠道菌群结构的差异分析[J].渔业研究,2019,41(3):187-194. [10] 杨求华,陆振,林琪,等.南移池塘养殖仿刺参肠道菌群结构分析[J].渔业研究,2016,38(3):192-201. [11] 王姣姣,李丹,王轶南,等.不同养殖时期刺参肠道内菌群结构的分析[J].大连海洋大学学报,2015,30(4):345-350. [12] SHA Y J, LIU M, WANG B J, et al. Gut bacterial diversity of farmed sea cucumbers Apostichopus japonicus with different growth rates[J].Microbiology,2016,85(1):109-115. [13] LIU B N, ZHOU W M, WANG H, et al. Bacillus baekryungensis MS1 regulates the growth, non-specific immune parameters and gut microbiota of the sea cucumber Apostichopus japonicus[J].Fish & Shellfish Immunology,2020,102:133-139. [14] WANG X D, JIANG J W, GUAN X Y, et al. Effects of Lactobacillus acidophilus and tussah immunoreactive substances on disease resistance of sea cucumber (Apostichopus japonicus) against Vibrio splendidus[J].Aquaculture Research,2021,52(10):4601-4612. [15] YANG G, TIAN X L, DONG S L. Bacillus cereus and rhubarb regulate the intestinal microbiota of sea cucumber (Apostichopus japonicus Selenka):species-species interaction, network, and stability[J].Aquaculture,2019,512:734284. [16] BOGATYRENKO E A, BUZOLEVA L S. Characterization of the gut bacterial community of the Japanese sea cucumber Apostichopus japonicus[J].Mikrobiologiia,2016,85(1):92-99. [17] 窦妍,丁君,曲凌云,等.秋、冬季刺参养殖池塘菌群的多样性分析[J].大连海洋大学学报,2015,30(2):143-148. [18] 王轶南,朱世伟,常亚青.刺参肠道及养殖池塘菌群组成的PCR-DGGE指纹图谱分析[J].渔业科学进展,2010,31(3):119-122. [19] 徐瀚晨.北方典型岸基刺参养殖池塘生态系统菌群结构特征与环境因子相关性分析[D].大连:大连海洋大学,2019. [20] POND M J, STONE D M, ALDERMAN D J. Comparison of conventional and molecular techniques to investigate the intestinal microflora of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2006,261(1):194-203. [21] DEEG C M, ZIMMER M M, GEORGE E E, et al. Chromulinavorax destructans, a pathogen of microzooplankton that provides a window into the enigmatic candidate Phylum Dependentiae[J].PLoS Pathogens,2019,15(5):e1007801. [22] GRAHAM E D, TULLY B J. Marine Dadabacteria exhibit genome streamlining and phototrophy-driven niche partitioning[J].The ISME Journal,2021,15(4):1248-1256. [23] 阳钢.几种微生态制剂对刺参(Apostichopus japonicus)养殖水体及刺参肠道菌群结构的影响[D].青岛:中国海洋大学,2012. [24] XU C, ZHONG X Q, LI X F, et al. Regulation of growth, intestinal microflora composition and expression of immune-related genes by dietary supplementation of Streptococcus faecalis in blunt snout bream (Megalobrama amblycephala)[J].Fish & Shellfish Immunology,2020,105:195-202. [25] 赵业,唐永政,李秉钧,等.四种典型抗生素对刺参幼参的急性毒性研究[J].海洋湖沼通报,2019(2):132-138. [26] 闫倩倩,李彬,廖梅杰,等.山东主要刺参养殖区幼参肠道抗生素耐药菌及耐药基因分布特征[J].渔业科学进展,2020,41(4):134-143. [27] 逄慧娟,廖梅杰,李彬,等.刺参(Apostichopus japonicus)保苗期“肠炎病”及其治疗方法[J].渔业科学进展,2017,38(3):188-197.
2023, Vol. 42 
