Effect of Tussah Immunoreactive Substances and Tussah Antibacterial Peptide on Growth, and Immunity of Yesso Scallop Patinopecten yessoensis
MA Shuhui1, TIAN Bin2, SUN Yongxin1, LI Xuejun1, MI Rui1, WEN Zhixin1, MENG Nan1, LI Yajie1, LI Shuying1, DU Xingfan1
1. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China; 2. Dalian Modern Agricultural Production Development Service Center, Dalian 116023, China
Abstract：Tussah immunoreactive substances (TIS) and tussah antibacterial peptide (TAP) are prepared by a specific process after the immune induction of tussah pupa. As natural antibacterial substances, they have been used in animal breeding and have made some effects. In order to study the effects of TIS and TAP on growth, immune-related enzyme activities and disease resistance of Yesso scallop, Patinopecten yessoensis, three hundred and twenty scallops with initial average body weight of (54.75±0.07) g were temporarily reared for one week and randomly divided into four treatment groups: control (provided with a diet of only micro-algae), tussah pupa powder supplementation (2% at the final concentration), TIS supplementation (2% at the final concentration), and TAP supplementation (2% at the final concentration) for 30 days. The results showed that the activities of lysozyme, acid phosphatase and superoxide dismutase were significantly higher in TAP supplementation treatment than those in the other three groups (P<0.05), with significant increase in the specific growth rate (P<0.05). There was no significant difference in catalase activity (P>0.05). Infectious diseases occurred during the experiment; the mortality rate of the TAP group was significantly lower than that in the control group (P<0.05). The activities of lysozyme and superoxide dismutase were decreased significantly in TP and TIS groups (P<0.05);the activities of acid phosphatase, superoxide dismutase and the specific growth rate were decreased compared with the control group (P>0.05);the mortality rate exhibited significant increase (P<0.05). Above all, the dietary of TAP at dose of 2% can improve growth, innate immunity of scallops and enhance the resistance, which can be used in Yesso scallop production and farming.
马淑慧, 田斌, 孙永欣, 李学军, 米锐, 温志新, 孟楠, 李亚洁, 李树英, 都兴范. 柞蚕免疫蛹粉、柞蚕抗菌肽对扇贝生长、免疫力的影响[J]. 水产科学, 2023, 42(5): 878-883.
MA Shuhui, TIAN Bin, SUN Yongxin, LI Xuejun, MI Rui, WEN Zhixin, MENG Nan, LI Yajie, LI Shuying, DU Xingfan. Effect of Tussah Immunoreactive Substances and Tussah Antibacterial Peptide on Growth, and Immunity of Yesso Scallop Patinopecten yessoensis. 水产科学, 2023, 42(5): 878-883.
[1] 常亚青.贝类增养殖学[M].北京:中国农业出版社,2007:91-99. [2] 迟庆宏.虾夷扇贝自然海域生态育苗技术[J].当代水产,2020,45(6):79. [3] KEYMANESH K, SOLTANI S, SARDARI S. Application of antimicrobial peptides in agriculture and food industry[J].World Journal of Microbiology and Biotechnology,2009,25(6):933-944. [4] BOMAN H G, NILSSON I, RASMUSON B. Inducible antibacterial defence system in Drosophila[J].Nature,1972,237(5352):232-235. [5] STEINER H, HULTMARK D, ENGSTRÖM Å, et al. Sequence and specificity of two antibacterial proteins involved in insect immunity[J].Nature,1981,292(5820):246-248. [6] VIZIOLI J, BULET P, CHARLET M, et al. Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae[J].Insect Molecular Biology,2000,9(1):75-84. [7] VANHOYE D, BRUSTON F, NICOLAS P, et al. Antimicrobial peptides from hylid and ranin frogs originated from a 150-million-year-old ancestral precursor with a conserved signal peptide but a hypermutable antimicrobial domain[J].European Journal of Biochemistry,2003,270(9):2068-2081. [8] VILJAKAINEN L, PAMILO P. Selection on an antimicrobial peptide defensin in ants[J].Journal of Molecular Evolution,2008,67(6):643-652. [9] TESSERA V, GUIDA F, JURETIĆ D, et al. Identification of antimicrobial peptides from teleosts and anurans in expressed sequence tag databases using conserved signal sequences[J].The FEBS Journal,2012,279(5):724-736. [10] BROGDEN K A. Antimicrobial peptides:pore formers or metabolic inhibitors in bacteria?[J].Nature Reviews Microbiology,2005,3(3):238-250. [11] 安春菊,盛长忠,李德森,等.一类潜在的新药——抗菌肽[J].中国新药杂志,2003,12(9):704-707. [12] MARR A K, GOODERHAM W J, HANCOCK R E. Antibacterial peptides for therapeutic use: obstacles and realistic outlook[J].Current Opinion in Pharmacology,2006,6(5):468-472. [13] 林鑫,毛述宏,杨阳,等.饲料中添加抗菌肽对锦鲤生长、非特异性免疫力和抗病力的影响[J].动物营养学报,2013,25(8):1860-1865. [14] DONG X Q, ZHANG D M, CHEN Y K, et al. Effects of antimicrobial peptides (AMPs) on blood biochemical parameters, antioxidase activity, and immune function in the common carp (Cyprinus carpio)[J].Fish & Shellfish Immunology,2015,47(1):429-434. [15] 覃志彪,梁静真,龙苏,等.抗菌肽对凡纳滨对虾生产性能及机体成分的影响[J].南方农业学报,2016,47(4):674-678. [16] 翟少伟,史庆超,陆鹏,等.不同抗菌脂肽添加物对吉富罗非鱼增重率、血清生化指标和非特异性免疫力的影响[J].广东农业科学,2015,42(21):129-133. [17] MA S H, SUN Y X, WANG F Q, et al. Effects of tussah immunoreactive substances on growth, immunity, disease resistance against Vibrio splendidus and gut microbiota profile of Apostichopus japonicus[J].Fish & Shellfish Immunology,2017,63:471-479. [18] 孙永欣,马淑慧,米锐,等.柞蚕免疫蛹粉在凡纳滨对虾健康养殖中的应用研究[J].中国饲料,2017(15):20-23. [19] 孙永欣,马淑慧,王庆志,等.饲喂柞蚕免疫蛹粉对红鳍东方鲀生长、免疫和抗病力的影响[J].水产科学,2022,41(2):236-242. [20] 都兴范.新型饲料蛋白质柞蚕免疫活性蛋白的研究应用[J].饲料研究,1997(11):3-4. [21] 马淑慧,李亚洁,孙永欣,等.柞蚕抗菌活性物质的抗菌性能及对仔猪腹泻大肠杆菌的抑菌效果研究[J].中国蚕业,2015,36(2):15-19. [22] 孙恩涛,秦志辉.昆虫抗菌肽研究进展[J].热带病与寄生虫学,2006,4(1):47-50. [23] 宋强,方建光,刘慧,等.沉积再悬浮颗粒物对3种滤食性贝类摄食生理的影响[J].海洋水产研究,2006,27(4):21-28. [24] 徐大伦,黄晓春,欧昌荣,等.浒苔多糖对扇贝SOD酶和溶菌酶活力的影响[J].水利渔业,2005,26(3):22-23. [25] 杨文鸽,薛长湖,徐大伦,等.β-1,3-葡聚糖及其羧甲基衍生物对海湾扇贝免疫功能的影响[J].水产学报,2006,30(2):236-240. [26] 陈超然,陈晓辉,陈昌福.口服甘草素对中华鳖稚鳖抗嗜水气单胞菌感染的作用[J].华中农业大学学报,2000,19(6):577-580. [27] YANG S P, WU Z H, JIAN J C, et al. Effect of marine red yeast Rhodosporidium paludigenum on growth and antioxidant competence of Litopenaeus vannamei[J].Aquaculture,2010,309(1/2/3/4):62-65. [28] 滕炜鸣,李文姬,张明,等.虾夷扇贝脓胞病病原的分离、鉴定与致病性[J].水产学报,2012,36(6):937-943. [29] MI R, LI X J, SUN Y X, et al. Effects of microbial community and disease resistance against Vibrio splendidus of Yesso scallop (Patinopecten yessoensis) fed supplementary diets of tussah immunoreactive substances and antimicrobial peptides[J].Fish & Shellfish Immunology,2022,121:446-455. [30] SHAN Z G, YANG Y P, GUAN N, et al. NKL-24:a novel antimicrobial peptide derived from zebrafish NK-lysin that inhibits bacterial growth and enhances resistance against Vibrio parahaemolyticus infection in Yesso scallop, Patinopecten yessoensis[J].Fish & Shellfish Immunology,2020,106:431-440.
2023, Vol. 42 
