Effects of Three Environmental Factors on Nitrogen Absorption of Filamentous alga Cladophora
WANG Luping1, TANG Yongtao1, WANG Chen1, TANG Boping2, CHENG Yongxu1
1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 2. School of Wetland, Yancheng Teachers University, Yancheng 224002, China
Abstract：Nitrogen is one of the important nutrients of aquatic plants. The utilization capacity of nitrogen plays an important role in the niche competition of aquatic plants. However, the utilization of nitrogen is also affected by environmental factors. In this study, we focused on Cladophora, which hasimportant influence on culture, aiming to explore the influence of environmental factors on Cladophora nitrogen absorption, and provide ideas for nitrogen removal or biomass control. In this experiment, NH4Cl was used as nitrogen source, and light incubator was used as culture condition. The nitrogen uptake of Cladophora was detected continuously. The time course and appropriate concentration for the stable absorption of NH4+-N in Cladophora were determined. Accordingly, orthogonal experiment of temperature, pH and light intensity L9(33) with different gradients was carried out. The results showed that Cladophora had a linear absorption of NH4+-N (R2>0.99) when the concentration of NH4+-N was 80 μmol/L within 0.5—1.5 h. The orthogonal experiment results showed that there wasa significant effect of temperature on the absorption of NH4+-N in Cladophora (P<0.05), whereas no significant effects for light intensity and pH (P>0.05). The effect of the above three factors on the absorption rate of NH4+-N in Cladophora was in such an order: temperature>pH> light intensity. The three-factor combination that was most conducive to the absorption in NH4+-N of Cladophora was temperature of 25 ℃, pH of 9, and light intensity of 4500 lx. The correlation analysis of various enzymes, total antioxidant capacity and ammonia nitrogen absorption rate of Cladophora showed that environmental factors influenced nitrogen absorption by various enzymes and non-enzymes. The finding provides a scientific basis for scientific research, the purification of sewage by Cladophora and the prediction and inhibition of the occurrence of filamentous algae such as Cladophora.
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2023, Vol. 42 
