Abstract：C1q, as the complement recognition protein of the classical activation pathway, is very important for the immunity of the body. In order to study the role of C1q gene in the immune defense of sea cucumber Apostichopus japonicus, the full-length cDNA sequence of sea cucumber C1q (AjC1q) cDNA was cloned by the homologous gene cloning and RACE, and other molecular biology techniques. The full-length cDNA sequence was 1717 bp, in which the 5'- terminal untranslated region was 110 bp, the 3-terminal untranslated region was 698 bp, and the open reading frame was 909 bp, encoding 302 amino acids. AjC1q protein has a globular C1q domain and is a trimer, which has high homology with the C1q domain of most animals in wild. The mRNA of AjC1q was expressed in the whole development of sea cucumber. The expression increased significantly in the doliolaria stage and reached the peak in the pentactula stage, indicating that the complement system may be widely activated in the doliolaria stage. And the mRNA of AjC1q was expressed in different tissues and was highly expressed in tube feet. In addition, the AjC1q gene could be up-regulated in a short time after stimulation by different pathogens, indicating that AjC1q is rapidly expressed under the stimulation of pathogens. So AjC1q may play an important role in the development of sea cucumber and in the immune response.
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