Changes in Muscle Quality and Physiochemical Properties of Grouper Hybrid Epinephelus fuscoguttatus ♂×E. lanceolatus ♀ during Keeping-alive with Water
WEN Jingjing1, WANG Lei1, WANG Jinxiang1, LI Xuepeng1, LI Jianrong1, DING Yuting2, XIE Jing3, WANG Yanbo4, QIN Xiaoming5
1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, China; 2. College of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, China; 3. College of Food Science, Shanghai Ocean University, Shanghai 201306, China; 4. School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China; 5. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
Abstract：The survival rate, body mass loss rate, general chemical composition and texture of muscle characteristics, muscle tissue morphology, serum biochemical indicators, fish gill Na+/K+-ATPase activity and tissue morphology were determined in pearl gentian grouper (Epinephelus fuscoguttatus♂×E. lanceolatus♀) with a body mass of (700±50) g placed in a 90 × 45 × 30 cm tank with continuous oxygenation for 0, 24, 48, 72, 96 and 120 h at a density of 100 g/L at a salinity of 24—26 and water temperature of (25±2) ℃to explore the changes in muscle quality and physiological and biochemical indicators at different water retention periods. The result showed that the survival rate of pearl gentian grouper was showed to be decreased, and body weight loss rate to be significantly increased (P<0.05), contents of crude fat and protein to be significantly decreased (P<0.05) with the time extended,while the hardness to be depressed gradually. With the extension of survival time, the morphology of muscles tissue was observed to be changed significantly, with increase in the gap between muscles fibers gradually, and the fractured structure of muscles tissue gradually. The activity of alanine aminotransferase and aspartate transaminase in fish blood was increased, while cortisol level went down firstly then up. There was decrease in Na+/K+-ATPase activity in gills and then increase. The lamellae became longer and thinner, accompanied by edema, and the whiteness of gills was increased significantly (P<0.05). The serum biochemical properties and the gills tissue morphology characteristics were expected to be potential indicators to characterize the of pearl gentian grouper during keeping-alive, which provided reference for the dynamic monitoring of the quality of live grouper during the live with water.
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2023, Vol. 42 
