Comparison of Nutrient Compositions and Flavor Substances between Hard Clam Meretrix meretrix and Northern Quahog Mercenaria mercenaria
CHEN Suhua1, CHEN Aihua1, WU Yangping1, ZHANG Yu1, CAO Yi1, ZHANG Zhidong1, LI Qiujie2, SUN Xuefeng3
1. Jiangsu Institute of Marine Fisheries, Nantong 226007,China; 2. College of Fisheries and Life, Shanghai Ocean University,Shanghai 201306, China; 3. College of Marine Science and Engineering, Nanjing Normal University, Nanjing 201123, China
Abstract:To evaluate the nutritional value of hard clam Meretrix meretrix and northern quahog Mercenaria mercenaria, the contents of approximate compositions,fatty acids, free amino acids,nucleotides, inorganic ions and volatile flavor substances were comparatively detected and analyzed in the soft body of the same month old hard clam(shell length 20.66±3.24 mm) and northern quahog(shell length 29.70 ± 2.23 mm) under the same aquaculture environment using the conventional nutritional determination methods and mass spectrometry. Results showed that there was significantly lower content of crude fat in hard clam than that in northern quahog (P< 0.05), with the mximal content of PUFA in hard clam (45.57%), especially EPA (13.29 %) and DHA (11.48 %), higher than those in northern quahog, without significant difference in contents of adenylate (AMP), guanylate (GMP), and inosinate (IMP) between the two clams. The three nucleotides exhibited major taste impacts on the hard clam, while IMP of northern quahog did not make much contribution to its taste flavor. The inorganic ions were all found to be the major flavor contributors in the two clams, with significantly higher contents of Na+, Fe2+, Zn+, Cl-and PO43-in hard clam than those in northern quahog(P<0.05), and significantly higher contents of Ca2+ and K+ in northern quahog than those in hard clam (P<0.05). There were 29 kinds of flavor-active compounds detected from hard clam and 42 kinds from northern quahog, with the maximal contents of alcohols in these two clams, indicating that alcohols play important roles in the overall odor. The odorant activity value (OAV) of 1-octen-3-ol in northern quahog was significantly higher than that in hard clam, which smelled “earthly” with excessive content.It was found that hard clam and northern quahog both had high nutritional value, a bit better nutrients and flavor substances in hard clam than northern quahog.
[1] 万宇.江苏南通文蛤产业结构与特征初步研究[D].青岛:中国海洋大学,2013:56-60. [2] 阙华勇,张国范.我国贝类产业技术的现状与发展趋势[J].海洋科学集刊,2016(1):69-76. [3] 张志东,陈爱华,吴杨平,等.文蛤红色选育系幼贝滤水率响应面法分析[J].水产科学,2020,39(3):431-437. [4] 高郡焕,李学鹏,陈桂芳,等.不同口味即食文蛤的加工工艺[J].食品工业科技,2014,35(11):216-219. [5] 林炫锋.硬壳蛤土池人工育苗技术[J].福建水产,2009,31(4):32-34. [6] 宋坚,常亚青.硬壳蛤在北方地区的引种、人工育苗及养成技术[J].中国水产,2009(9):50-51. [7] 李建军,潘元潮.美国硬壳蛤池塘混养技术[J].养殖与饲料,2019(10):54-55. [8] 张安国,李太武,苏秀榕,等.不同地理种群文蛤的营养成分研究[J].水产科学,2006,25(2):79-81. [9] 顾向飞,林志华,董迎辉,等.3种壳色花纹文蛤常规营养成分分析与评价[J].动物营养学报,2014,26(12):3850-3857. [10] 杨晋,陶宁萍,王锡昌.文蛤的营养成分及其对风味的影响[J].中国食物与营养,2007,13(5):43-45. [11] 王超,陈爱华,姚国兴,等.4个文蛤群体鲜味物质比较分析[J].海洋科学,2016,40(9):45-52. [12] WEN X Y, CHEN A H, WU Y P, et al. Comparative evaluation of proximate compositions and taste attributes of three Asian hard clams (Meretrix meretrix) with different shell colors[J].International Journal of Food Properties,2020,23(1):400-411. [13] 李苹苹.五种经济贝类的营养成分及蛋白质质量分析[J].食品研究与开发,2014,35(15):99-101. [14] 李娜.贝类中氨基酸、脂肪酸和重金属的含量分析及其产品质量评价[D].保定:河北农业大学,2011:5-7. [15] 杨建敏,邱盛尧,郑小东,等.美洲帘蛤软体部营养成分分析及评价[J].水产学报,2003,27(5):495-498. [16] 张思嘉,陈蓉,孔周雁,等.海水净化过程中球等鞭金藻对硬壳蛤风味品质的影响[J].核农学报,2021,35(1):147-158. [17] 中华人民共和国国家卫生和计划生育委员会.GB 5009.3—2016,食品安全国家标准 食品中水分的测定[S].北京:中国标准出版社,2017. [18] 中华人民共和国国家卫生和计划生育委员会.GB 5009.4—2016,食品安全国家标准 食品中灰分的测定[S].北京:中国标准出版社,2017. [19] 国家卫生和计划生育委员会,国家食品药品监督管理总局.GB 5009.6—2016,食品安全国家标准 食品中脂肪的测定[S].北京:中国标准出版社,2017. [20] 国家卫生和计划生育委员会,国家食品药品监督管理总局.GB 5009.5—2016,食品安全国家标准 食品中蛋白质的测定[S].北京:中国标准出版社,2017. [21] 国家卫生和计划生育委员会,国家食品药品监督管理总局.GB 5009.168—2016,食品安全国家标准 食品中脂肪酸的测定[S].北京:中国标准出版社,2017. [22] 国家卫生和计划生育委员会,国家食品药品监督管理总局.GB 5009.268—2016,食品安全国家标准 食品中多元素的测定[S].北京:中国标准出版社,2017. [23] 国家卫生和计划生育委员会,国家食品药品监督管理总局.GB 5009.87—2016,食品安全国家标准 食品中磷的测定[S].北京:中国标准出版社,2017. [24] 中华人民共和国国家卫生和计划生育委员会.GB 5009.44—2016,食品安全国家标准 食品中氯化物的测定[S].北京:中国标准出版社,2017. [25] WEN X Y, CHEN A H, XU Y S, et al. Comparative evaluation of volatile profiles of Asian hard clams (Meretrix meretrix) with different shell colors by electronic nose and GC-MS[J].Journal of Aquatic Food Product Technology,2021,30(1):107-121. [26] 顾赛麒,吴娜,张晶晶,等.MMSE-GC-O结合OAV法鉴定蒸制崇明地区中华绒螯蟹中关键气味物质[J].食品安全质量检测学报,2014,5(3):877-888. [27] 张殿福,吴雷,张学振,等.大西洋鲑、三倍体虹鳟和金鳟的肌肉营养成分与品质特性[J].中国水产科学,2020,27(2):186-194. [28] 夏天宇,冯若楠,张甜甜,等.脂肪酸与人类健康[J].畜牧兽医杂志,2018,37(3):32-33. [29] 韩迎雪,林婉玲,杨少玲,等.15种淡水鱼肌肉脂肪含量及脂肪酸组成分析[J].食品工业科技,2018,39(20):217-222. [30] 朱曦淳,张夜路,康继霞,等.5种经济鱼类内脏脂肪酸含量及组成分析[J].食品研究与开发,2021,42(4):22-27. [31] OGURO A, ISHIHARA Y, SISWANTO F M, et al. Contribution of DHA diols (19, 20-DHDP) produced by cytochrome P450s and soluble epoxide hydrolase to the beneficial effects of DHA supplementation in the brains of rotenone-induced rat models of Parkinson′s disease[J].Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids,2021,1866(2):158858. [32] PASSI S, CATAUDELLA S, DI MARCO P, et al. Fatty acid composition and antioxidant levels in muscle tissue of different Mediterranean marine species of fish and shellfish[J].Journal of Agricultural and Food Chemistry,2002,50(25):7314-7322. [33] FUKE S, KONOSU S. Taste-active components in some foods:a review of Japanese research[J].Physiology & Behavior,1991,49(5):863-868. [34] ZHANG Y, VENKITASAMY C, PAN Z L, et al. Recent developments on umami ingredients of edible mushrooms - a review[J].Trends in Food Science & Technology,2013,33(2):78-92. [35] VENUGOPAL V, GOPAKUMAR K. Shellfish:nutritive value, health benefits, and consumer safety[J].Comprehensive Reviews in Food Science and Food Safety,2017,16(6):1219-1242. [36] 罗红宇,王斌,冯刚,等.用ICP-MS法分析3种魟鱼和2种鲨鱼软骨中矿物元素含量[J].营养学报,2011,33(5):529-530. [37] 王斌,邬华威,李龙岩.金枪鱼鱼卵营养成分分析及营养评价[J].广东海洋大学学报,2020,40(2):111-116. [38] 石丽荣.长竹蛏营养成分及微量元素含量测定与分析[J].闽南师范大学学报(自然科学版),2020,33(3):43-47. [39] HAYASHI T, YAMAGUCHI K, KONOSU S. Sensory analysis of taste-active components in the extract of boiled snow crab meat[J].Journal of Food Science,1981,46(2):479-483. [40] 毛羽扬.咸味、鲜味和咸鲜调味平台的建立[J].中国调味品,2001,26(12):25-27. [41] 翁世兵,孙恢礼.海产鲜味物质及海产品特征滋味的研究进展[J].中国调味品,2007,32(11):21-27. [42] 柳敏.白鲢鱼肉挥发性风味物质分析及其与蛋白质结合研究[D].杭州:浙江工业大学,2015:20-33. [43] 王建华,葛敏敏.室温贮藏过程中南美白对虾挥发性物质的变化[J].食品科技,2019,44(1):187-193. [44] 田淑琳,周文红,刘小玲,等.基于GC-O-MS和ROAV法的马氏珍珠贝挥发性风味成分及腥味特征物质分析[J].大连海洋大学学报,2019,34(4):573-579. [45] 顾聆琳.牡蛎中风味物质的研究及调味料的制备[D].无锡:江南大学,2005:20-21. [46] RAN Z S, ZHANG S J, ZHU Y L, et al. Effect of salinity on volatiles in the razor clam investigated by head space-solid phase microextraction/gas chromatography-mass spectrometry[J].Fisheries Science,2019,85(1):137-146. [47] FRATINI G, LOIS S, PAZOS M, et al. Volatile profile of Atlantic shellfish species by HS-SPME GC/MS[J].Food Research International,2012,48(2):856-865. [48] 蔡原,刘哲,宋明伟,等.虹鳟不同部位鱼肉挥发性风味物质组成比较[J].食品科学,2011,32(16):269-273. [49] 章超桦,平野敏行,铃木健,等.鲫的挥发性成分[J].水产学报,2000,24(4):354-358. [50] 吴靖娜,路海霞,刘智禹,等.用电子鼻和SPME-GC-MS分析鲍鱼熟制前后挥发性风味物质的变化[J].大连海洋大学学报,2016,31(4):431-437.