Genetic Mechanisms Underlying Inbreeding Depression of F1 Generation in Yesso Scallop Patinopecten yessoensis
ZHAO Liang1,2,3,4, LI Yangping4, GAO Shan1,2,3, JIANG Pingzhe1,2,3, LIN Shanshan1,2,3, ZHANG Guohan1,2,3, FAN Guangqi1,2,3, JIANG Jingwei1,2,3, ZHOU Zunchun1,2,3
1. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China; 2. Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Dalian 116023, China; 3. Key Laboratory of Germplasm Improvement and Fine Seed Breeding for Marine Aquatic Animals, Liaoning Province, Dalian 116023, China; 4. Ocean University of China, Qingdao 266100, China
Abstract：In order to clarify phenomenon and genetic regulatory mechanism of inbreeding depression in yesso scallop Patinopecten yessoensis in the phenotypic level, experimental groups and control groups of yesso scallop with inbreeding coefficients of 0.5, 0.25, and 0 were constructed. At the same time,seven fitness traits were selected through tracking from the larval stage to the 530 days old adult stage, and a fitness index evaluation method based on inbreeding depression was constructed using principal component analysis.In the genomic level, whole-genome resequencing was performed on the parents of a self-fertilization lineage and 21 offsprings for segregation distortion analysis. It was found that the inbreeding depression rate in the self-fertilization group was 1.31—3.05 times during the larval stage,higher than that in the inbred group. In the adult stage, the trend was similar to that in the larval stage, with the inbreeding depression rate of 0.97—7.29 times in the self-fertilization group, higher than that in the inbred group. However, only the self-fertilization group maintained a low inbreeding depression rate (1.65%—2.56%) in growth traits during the larval stage. In the adult stage, the self-fertilization group and most of the inbred groups did not experienced any depression, indicating that inbreeding depression mainly occurs in survival traits. A total of 1 253 519 SNPs were genotyped for biased segregation analysis, with 99% of the SNPs exhibiting significant segregation distortion in the offspring, 23% of gametic segregation, and 77% of zygotic segregation. In zygotic segregation, 89% was due to heterozygote excess, indicating that overdominance effect was at work, and only 10% was affected by dominant effects as a result of homozygote loss. The findings indicate that overdominance effect and segregation distortion play a dominant role in inbreeding depression of yesso scallop F1, not only explores biased segregation in self-fertilized offspring of yesso scallop, but also provide important reference for the genetic mechanism of inbreeding depression in aquatic animals.
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2024, Vol. 43 
