Analysis and Expression of SIRT Gene Family in Gymnocypris eckloni Exposed to Low-Temperature
NI Weilin1,2, LIU Dan1, GAO Qiang1, CHAO Yan2, ZHANG Cunfang1, KOU Ruobin1,3, QI Delin1, NIE Miaomiao1
1. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China; 2. Animal Science Department of Agriculture and Animal Husbandry College, Qinghai University, Xining 810016, China; 3. College of Eco-Environmental Engineering, Qinghai University, Xining 810016, China
Abstract：In order to explore the role of genes of SIRT (sirtuins) gene family in the low temperature adaptation in Gymnocypris eckloni, the bioinformatics and gene expression of seven genes of the SIRT gene family were analyzed in 2.5 years old G. eckloni with 14—20 cm in body length.The results showed that there was significantly different length of SIRTs in G. eckloni, with the longest SIRT1 gene (22 885 bp), and the shortest SIRT4 gene (3660 bp), and the number of amino acids encoded by SIRT gene family was ranged from 304 to 691. The results of conserved domain and motif analyses revealed that they all belonged to the Sir2 superfamily and contained conserved motifs, such as GAGxSx, xxPxxR, PxxxH and QNxDxLx. The predicted amino acid physicochemical properties displayed that all the SIRT proteins of the G. eckloni were hydrophilic proteins,and the stability of SIRTs proteins were predicted to be low except SIRT4. The predicted results of subcellular localization showed that SIRT proteins were primarily distributed in the cytoplasm and nucleus.SIRTs were found to interact with SOD2, CAT, PPARGC1α, p53, FOXO1a and FOXO1b by predicting protein interactions. The expression of SIRTs in liver, brain and muscle of cold-adapted G. eckloni, the results showed that SIRT2, SIRT4 and SIRT7 were highly expressed in liver and brain, and SIRT2—4, SIRT6 and SIRT7 were significantly up-regulated in muscle. So, SIRTs,especially, SIRT2, SIRT4 and SIRT7 of G. eckloni may play an important role in cold environment. These findings introduce the characteristics of the SIRT gene family in G. eckloni, and provide a basis for subsequent research on the role of SIRTs under cold adaptation in G. eckloni.
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2024, Vol. 43 
