Effects of Water Temperature on Distribution and Type of Skin Mucus Cells and Mucus Immune Factors of Mandarinfish Siniperca chuatsi
SHI Yusong, ZHAO Jinliang
Key Laboratory of Freshwater Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Shanghai Collaborative Innovation Center, Aquatic Animal Genetics and Breeding Center, National Experimental Teaching Demonstration Center for Fishery Science, Shanghai Ocean University, Shanghai 201306, China
Abstract：In order to understand the effects of different temperatures on the surface mucus cell types and mucus immune factors of mandarinfish Siniperca chuatsi, the mandarinfish with body weight of (19.0±3.2) g and body length of (11.0±1.5) cm was placed in an aquarium with water temperature of 10, 20 and 30 ℃, and at 0, 36 and 72 h, the excess water on the body surface of the mandarin was gently wiped off with absorbent paper, the mucus on the trunk and tail was gently scraped with a glass slide, and the skins of head, trunk and caudal peduncle was immediately cut off from the mandarinfish anaesthetized with 1% MS-222 on ice, and fixed in Bonn′s fluid to observe the effects of three temperatures on the distribution and types of body surface mucus cells at 72 h after the experiment by AB-PAS staining method. The staining results showed that there were four types of mucus cells on the skin surface of mandarinfish. The maximal number of mucus cells per unit area was observed in skin of the head, followed by the trunk and the caudal peduncle. The type Ⅱ was found to be increased the most in 20 ℃ and 30 ℃ groups, followed by type Ⅳ compared with 10 ℃ group, type Ⅰ showing slightly decreasing trend, and type Ⅲ showing irregular change. qRT-PCR and double-antibody sandwich enzyme-linked immunoassay revealed that the expression levels of IgM gene and Hepcidin gene were up-regulated with increasing temperature. The expression levels of IL-1β gene were significantly up-regulated in the 20 ℃ group and down-regulated in the 30 ℃ group compared with the 10 ℃ group. There was significantly higher SOD activity in 30 ℃ group than that in 10 ℃ and 20 ℃ groups, while the ACP and LYS activities were slightly higher than those in 10 ℃ and 20 ℃ groups. The findings indicate that the skin mucus cell types and number and immune-related factors of mandarinfish are significantly affected by ambient temperature.
施郁松, 赵金良. 温度对鳜体表黏液细胞及黏液免疫因子的影响[J]. 水产科学, 2024, 43(1): 32-40.
SHI Yusong, ZHAO Jinliang. Effects of Water Temperature on Distribution and Type of Skin Mucus Cells and Mucus Immune Factors of Mandarinfish Siniperca chuatsi. 水产科学, 2024, 43(1): 32-40.
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2024, Vol. 43 
