Comparative Analysis of Genetic Variation between Wild and Cultured Populations of Platypharodon extremus Based on Mitochondrial DNA D-loop Sequence
YANG Zhuoyu1, DU Yanyan1, KA Wei1, JIAO Wenlong1, WANG Tai1, ZHANG Yanping1, DA Hu2
1. Gansu Key Laboratory of Cold-Water Fishes Genetic Resources & Breeding, Gansu Fisheries Research Institute, Lanzhou 730030, China; 2. Gansu Computing Center, Lanzhou 730030, China
Abstract：In this study, the mitochondrial DNA control region (D-loop) gene sequences were analyzed in 21 samples of wild Platypharodon extremus collected from Maqu County in Gansu Province and Maduo in Qinghai Province and 39 samples of F1 and 38 samples of F2 cultured P. extremus in Linxia, Gansu Province. The results showed that there were high haplotype diversity (Hd=0.950±0.011) and low nucleotide diversity (Pi=0.00987±0.00041) in the four populations, with the higher haplotype number (Nh), haplotype diversity index (Hd) and nucleotide diversity index (Pi) in the wild population than those in the cultured population. The analysis of genetic structure showed there was genetic differentiation between the wild population and cultured population. The neutral test revealed that the two wild populations had population expansion events. In conclusion, the cultured populations slightly showed the reduce in genetic diversity due to effect of the limited number of breeding fish and artificial selection. So it is necessary to evaluate the effect of breeding and releasing regularly and use scientific artificial breeding methods to enrich the genetic diversity.
杨濯羽, 杜岩岩, 卡伟, 焦文龙, 王太, 张艳萍, 达虎. 基于线粒体DNA控制区序列的极边扁咽齿鱼群体遗传研究[J]. 水产科学, 2024, 43(1): 79-87.
YANG Zhuoyu, DU Yanyan, KA Wei, JIAO Wenlong, WANG Tai, ZHANG Yanping, DA Hu. Comparative Analysis of Genetic Variation between Wild and Cultured Populations of Platypharodon extremus Based on Mitochondrial DNA D-loop Sequence. 水产科学, 2024, 43(1): 79-87.
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2024, Vol. 43 
