Abstract：The benthic diatoms are highly nutritious and essential food for sea cucumber Apostichopus japonicus in breeding.Effects of the environmental factors(pH, light intensity) and nutritional conditions (ratio of N and P, auxin concentrations of zeatin) on the growth of isolated Navicula seminulum were optimized in this study. The results showed that the optimum initial pH of medium was 8.0, the optimum light intensity was 2500 lx, the optimum zeatin concentration was 1.0 mg/L, and the optimum ratio of N and P was 12.5. Under these conditions, the highest specific growth rate and cell density of N. seminulum were acquired, and the biomass accumulation reached the maximum values. A feeding experiment was also conducted to investigate the effects of the N. seminulum on growth, digestive enzyme activity and immune defense factors of juvenile sea cucumber.The sea cucumber were fed the commercial feed, Cylindrotheca fusiformis, or the N. seminulum, respectively. During the 30-day feeding period, the activities of digestive and immune-related enzymes were determined every ten days.The results showed that the sea cucumbers fed N. seminulum not only promoted the growth performance, but also produced the maximum digestive and immune activities compared with C. fusiformis and commercial feed groups. These data collectively confirmed that the health-promoting effects were more obvious by applying N. seminulum. Dietary N. seminulum could effectively enhanced digestive function and immune response, depress oxidative damage, and improved the phosphatase-responsiveness and organismal defense capability. The findings may provide theoretical guidance for culture and production of marine benthic diatoms of sea cucumber farming industry.
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2024, Vol. 43 
