Effects of Florfenicol Residues on Growth and Antioxidant System of Chlorella sp.
WANG Baolong1, ZHANG Tao1,2, ZHANG Weixiao1,2, HUANG Ruipin1, MA Pengfei1,2, LI Na1,2, ZHANG Yingqi1, XUE Mingyu2, ZHANG Qian1,2, LIU Ying1
1. Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian 116023, China; 2. College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China
Abstract：With the intensive development of aquaculture, antibiotic residues in aquaculture effluent posed a serious threat to aquatic ecosystem, which has aroused global attention. In present study, the commonly used diet microalga Chlorella sp. in aquaculture was taken as the research object to explore the effects of florfenicol on marine microalgae. The effects of florfenicol exposure at different concentrations for 72 h on the growth, the contents of pigments, reactive oxygen species (ROS), malondialdehyde (MDA) content and antioxidant enzyme activities of Chlorella sp. were analyzed. The results showed that florfenicol at low concentration promoted the growth of Chlorella sp., while inhibited the growth of Chlorella sp. at high concentration, and the contents of chlorophyll varied in a reverse U-shaped trend with the increase in mass concentration of florfenicol, with the maximal chlorophyll content in 0.100 mg/L florfenicol group, which was 74.6% higher than that in control group. Florfenicol exposure may cause the oxidative damage to the cell membrane of microalgae, 20 mg/L florfenicol led to the increase of MDA contents and stimulates the carotenoids synthesis, was 2.45 times higher than that in the control group, which may participate in the resistance to oxidative damage. Under the experimental concentrations, the activities of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were significantly inhibited. Compared to Isochrysis galbana, Chlorella sp. has a high tolerance to florfenicol. The finding provides theoretical basis for the treatment of antibiotic residues in aquaculture effluent.
[1] 郭睿,张莺脐,张羡宇,等.氟苯尼考在海水鱼循环水养殖系统中的迁移转化规律[J].水产科学,2020,39(3):332-340. [2] WANG X D, YIN R L, ZENG L X, et al. A review of graphene-based nanomaterials for removal of antibiotics from aqueous environments[J]. Environmental Pollution,2019,253:100-110. [3] XU W H, ZHANG G, LI X D, et al. Occurrence and elimination of antibiotics at four sewage treatment plants in the Pearl River Delta (PRD), south China[J]. Water Research,2007,41(19):4526-4534. [4] 潘红艳,郭娇娇,杨虎,等.氟苯尼考在鲟鱼体内的药物代谢动力学研究[J].水产科学,2011,30(7):373-377. [5] REN X Y, WANG Z Q, GAO B Q, et al. Effects of florfenicol on the antioxidant status, detoxification system and biomolecule damage in the swimming crab (Portunus trituberculatus)[J]. Ecotoxicology and Environmental Safety,2017,143:6-11. [6] KIM Y, JUNG J, KIM M, et al. Prioritizing veterinary pharmaceuticals for aquatic environment in Korea[J]. Environmental Toxicology and Pharmacology,2008,26(2):167-176. [7] ZONG H M, MA D Y, WANG J Y, et al. Research on florfenicol residue in coastal area of Dalian (northern China) and analysis of functional diversity of the microbial community in marine sediment[J]. Bulletin of Environmental Contamination and Toxicology,2010,84(2):245-249. [8] GULLBERG E, CAO S, BERG O G, et al. Selection of resistant bacteria at very low antibiotic concentrations[J]. PLoS Pathogens,2011,7(7):e1002158. [9] 折雨亭.磺胺嘧啶对羊角月牙藻的毒性效应研究[D].太原:山西大学,2021:7-34. [10] 万禁禁.淡水微藻对几种抗生素胁迫的响应研究[D].泉州:华侨大学,2014:11-61. [11] 徐冬梅,王艳花,饶桂维.四环素类抗生素对淡水绿藻的毒性作用[J].环境科学,2013,34(9):3386-3390. [12] TOM A P, JAYAKUMAR J S, BIJU M N, et al. Aquaculture wastewater treatment technologies and their sustainability:a review[J]. Energy Nexus,2021,4:100022. [13] 翟晓嵌.海水养殖调水小球藻漂浮反应器大规模培养[D].大连:大连理工大学,2020:6-8. [14] 宗虎民.氟苯尼考对近岸沉积物中生源要素生物地球化学循环的影响[D].大连:大连海事大学,2008:98-102. [15] CARUSSO S, JUÁREZ A B, MORETTON J, et al. Effects of three veterinary antibiotics and their binary mixtures on two green alga species[J]. Chemosphere,2018,194:821-827. [16] ZHAI X Q, ZHU C B, ZHANG Y C, et al. Seawater supplemented with bicarbonate for efficient marine microalgae production in floating photobioreactor on ocean:a case study of Chlorella sp.[J]. Science of the Total Environment,2020,738:139439. [17] MEINERTZ J R, HESS K R, BERNARDY J A, et al. Florfenicol residues in rainbow trout after oral dosing in recirculating and flow-through culture systems[J]. Journal of Aquatic Animal Health,2014,26(4):243-250. [18] 欧丹云,陈彬,陈灿祥,等.九龙江下游河口水域抗生素及抗性细菌的分布[J].中国环境科学,2013,33(12):2243-2250. [19] ZHAO Y R, TANG X X, QUIGG A, et al. The toxic mechanisms of BDE-47 to the marine diatom Thalassiosira pseudonana-a study based on multiple physiological processes[J]. Aquatic Toxicology,2019,212:20-27. [20] WAN JJ, GUO P Y, PENG X F, et al. Effect of erythromycin exposure on the growth, antioxidant system and photosynthesis of Microcystis flos-aquae[J]. Journal of Hazardous Materials,2015,283:778-786. [21] RITCHIE R J. Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents[J]. Photosynthesis Research,2006,89(1):27-41. [22] QIAN H F, LI J J, SUN L W, et al. Combined effect of copper and cadmium on Chlorella vulgaris growth and photosynthesis-related gene transcription[J]. Aquatic Toxicology,2009,94(1):56-61. [23] BELIAEFF B, BURGEOT T. Integrated biomarker response:a useful tool for ecological risk assessment[J]. Environmental Toxicology and Chemistry,2002,21(6):1316-1322. [24] BROEG K, LEHTONEN K K. Indices for the assessment of environmental pollution of the Baltic Sea coasts:integrated assessment of a multi-biomarker approach[J]. Marine Pollution Bulletin,2006,53(8/9):508-522. [25] LIU W H, MING Y, HUANG Z W, et al. Impacts of florfenicol on marine diatom Skeletonema costatum through photosynthesis inhibition and oxidative damages[J]. Plant Physiology and Biochemistry,2012,60:165-170. [26] 连鹏,葛利云,邓欢欢,等.两种喹诺酮类抗生素对亚心形扁藻的毒性效应研究[J].环境科学与管理,2014,39(5):46-48. [27] 刘祚屹,刘伟杰,寇杰锋,等.硫酸锌霉素和盐酸环丙沙星对4种赤潮藻种群增长的影响[J].湖南师范大学自然科学学报,2019,42(6):23-31. [28] DING J N, LU G H, LIU J C, et al. Evaluation of the potential for trophic transfer of roxithromycin along an experimental food chain[J]. Environmental Science and Pollution Research,2015,22(14):10592-10600. [29] 官奕宏,李红卫,吕谋.诺氟沙星和四环素对铜绿微囊藻的毒性研究[J].四川环境,2015,34(5):25-30. [30] WANG G H, CHEN K, CHEN L Z, et al. The involvement of the antioxidant system in protection of desert Cyanobacterium Nostoc sp. against UV-B radiation and the effects of exogenous antioxidants[J]. Ecotoxicology and Environmental Safety,2008,69(1):150-157. [31] 潘慧云,李小路,徐小花,等.烯效唑对沉水植物伊乐藻(Elodea nuttallii)生长及抗氧化酶活性的影响[J].生态学报,2008,28(12):6159-6164. [32] ZHANG Y Q, ZHANG X Y, GUO R, et al. Effects of florfenicol on growth, photosynthesis and antioxidant system of the non-target organism Isochrysis galbana[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2020,233:108764. [33] LIU Y, CHEN S, ZHANG J A, et al. Stimulation effects of ciprofloxacin and sulphamethoxazole in Microcystis aeruginosaand isobaric tag for relative and absolute quantitation-based screening of antibiotic targets[J]. Molecular Ecology,2017,26(2):689-701. [34] 姜思,刘莹莹,佟少明.4种常用抗生素对莱茵衣藻生长及光化学活性的影响[J].生物加工过程,2017,15(2):13-20. [35] MACHADO M D, SOARES E V. Impact of erythromycin on a non-target organism:cellular effects on the freshwater microalga Pseudokirchneriella subcapitata[J]. Aquatic Toxicology,2019,208:179-186. [36] WANG Y, LI J H, LEI Y, et al. Bioremediation of sulfonamides by a microalgae-bacteria consortium - analysis of pollutants removal efficiency, cellular composition, and bacterial community[J]. Bioresource Technology,2022,351:126964. [37] 梁英,冯力霞,尹翠玲,等.叶绿素荧光技术在微藻环境胁迫研究中的应用现状及前景[J].海洋科学,2007,31(1):71-76. [38] RAMMUNI M N, ARIYADASA T U, NIMARSHANA P H V, et al. Comparative assessment on the extraction of carotenoids from microalgal sources astaxanthin from H. pluvialis and β-carotene from D. salina[J]. Food Chemistry,2019,277:128-134. [39] DI LENA G, CASINI I, LUCARINI M, et al. Carotenoid profiling of five microalgae species from large-scale production[J]. Food Research International,2019,120:810-818. [40] LI M, HU C W, ZHU Q, et al. Copper and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in the microalga Pavlova viridis (Prymnesiophyceae)[J]. Chemosphere,2006,62(4):565-572. [41] KERKSICK C, WILLOUGHBY D. The antioxidant role of glutathione and N-acetyl-cysteine supplements and exercise-induced oxidative stress[J]. Journal of the International Society of Sports Nutrition,2005,2(2):38-44. [42] FOYER C H, THEODOULOU F L, DELROT S. The functions of inter- and intracellular glutathione transport systems in plants[J]. Trends in Plant Science,2001,6(10):486-492. [43] SINGH R, UPADHYAY A K, CHANDRA P, et al. Sodium chloride incites reactive oxygen species in green algae Chlorococcum humicola and Chlorella vulgaris:implication on lipid synthesis, mineral nutrients and antioxidant system[J]. Bioresource Technology,2018,270:489-497. [44] LIU Z W, WANG Q, ZOU D H, et al. Effects of selenite on growth, photosynthesis and antioxidant system in seaweeds, Ulva fasciata (Chlorophyta) and Gracilaria lemaneiformis (Rhodophyta)[J]. Algal Research,2018,36:115-124. [45] CHEN S, ZHANG W, LI J Y, et al. Ecotoxicological effects of sulfonamides and fluoroquinolones and their removal by a green alga (Chlorella vulgaris) and a cyanobacterium (Chrysosporum ovalisporum)[J]. Environmental Pollution,2020,263:114554. [46] MURUSSI C R, COSTA M, MENEZES C, et al. Integrated assessment of biomarker response in carp (Cyprinus carpio) and silver catfish (Rhamdia quelen) exposed to clomazone[J]. Archives of Environmental Contamination and Toxicology,2015,68(4):646-654. [47] PYTHAROPOULOU S, SAZAKLI E, GRINTZALIS K, et al. Translational responses of Mytilus galloprovincialis to environmental pollution:integrating the responses to oxidative stress and other biomarker responses into a general stress index[J]. Aquatic Toxicology,2008,89(1):18-27. [48] 李磊,刘金殿,黄士林,等.基于综合生物标志物响应评价苯并芘对脊尾白虾(Exopalaemon carinicauda)的毒性效应[J].海洋与湖沼,2018,49(2):444-451.
2024, Vol. 43 
